Taiwan Cinnamomum kanehirae Hay vegetative propagation technology
A technology of asexual reproduction and camphor, which is applied in the field of plant tissue culture, can solve the problem of short supply of cattle-like seedlings in Taiwan, and achieve the effect of promoting the application in the mainland
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Embodiment 1
[0017] (1) Disinfection of explants: Select the budding stems of Cinnamomum camphora, semi-lignified, robust, and free from diseases and insect pests in the same year, rinse them under running water for 1 hour, soak in 3% washing powder solution for 5 minutes, and use a soft brush to wash 3% Scrub the material gently with detergent solution, then rinse with tap water in drips for 60 minutes. Soak in 75% ethanol solution for 45 seconds in the ultra-clean workbench, rinse with sterile water for 3 times, then disinfect with 0.1% mercury solution for 5 minutes, then rinse the explants with sterile water until there is no foam, and dry them with sterile filter paper After surface moisture, set aside.
[0018] (2) Induction culture: cut out the budded stem segment of the explant obtained in step (1) and inoculate it into the induction medium for adventitious bud induction culture. After inoculation, it was placed in the light for 13 hours a day, the light intensity was 1000lx, the ...
Embodiment 2
[0023] (1) Disinfection of explants: Select the budding stems of Cinnamomum camphora, semi-lignified, robust, and free from diseases and insect pests in the same year, rinse them under running water for 3 hours, soak in 3% washing powder solution for 7 minutes, and use a soft brush to wash 3% Scrub the material gently with detergent solution, then rinse with tap water for 80 minutes in drips. Soak in 75% ethanol solution for 50s in an ultra-clean workbench, rinse with sterile water for 5 times, then disinfect with 0.1% mercuric acid solution for 7 minutes, then rinse the explants with sterile water until there is no foam, and dry them with sterile filter paper After surface moisture, set aside.
[0024] (2) Induction culture: cut out the budded stem segment of the explant obtained in step (1) and inoculate it into the induction medium for adventitious bud induction culture. After inoculation, it was placed in the light for 14 hours a day, the light intensity was 1500lx, the c...
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