Taiwan Cinnamomum kanehirae Hay vegetative propagation technology

A technology of asexual reproduction and camphor, which is applied in the field of plant tissue culture, can solve the problem of short supply of cattle-like seedlings in Taiwan, and achieve the effect of promoting the application in the mainland

Inactive Publication Date: 2015-06-10
朱炳贵
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, due to the introduction of Taiwan cinnamon in many places in the mai

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Disinfection of explants: Select the budding stems of Cinnamomum camphora, semi-lignified, robust, and free from diseases and insect pests in the same year, rinse them under running water for 1 hour, soak in 3% washing powder solution for 5 minutes, and use a soft brush to wash 3% Scrub the material gently with detergent solution, then rinse with tap water in drips for 60 minutes. Soak in 75% ethanol solution for 45 seconds in the ultra-clean workbench, rinse with sterile water for 3 times, then disinfect with 0.1% mercury solution for 5 minutes, then rinse the explants with sterile water until there is no foam, and dry them with sterile filter paper After surface moisture, set aside.

[0018] (2) Induction culture: cut out the budded stem segment of the explant obtained in step (1) and inoculate it into the induction medium for adventitious bud induction culture. After inoculation, it was placed in the light for 13 hours a day, the light intensity was 1000lx, the ...

Embodiment 2

[0023] (1) Disinfection of explants: Select the budding stems of Cinnamomum camphora, semi-lignified, robust, and free from diseases and insect pests in the same year, rinse them under running water for 3 hours, soak in 3% washing powder solution for 7 minutes, and use a soft brush to wash 3% Scrub the material gently with detergent solution, then rinse with tap water for 80 minutes in drips. Soak in 75% ethanol solution for 50s in an ultra-clean workbench, rinse with sterile water for 5 times, then disinfect with 0.1% mercuric acid solution for 7 minutes, then rinse the explants with sterile water until there is no foam, and dry them with sterile filter paper After surface moisture, set aside.

[0024] (2) Induction culture: cut out the budded stem segment of the explant obtained in step (1) and inoculate it into the induction medium for adventitious bud induction culture. After inoculation, it was placed in the light for 14 hours a day, the light intensity was 1500lx, the c...

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PUM

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Abstract

The invention discloses Taiwan Cinnamomum kanehirae Hay vegetative propagation technology. The Cinnamomum kanehirae Hay belongs to the Lauraceae Cinnamomum plants, is the unique evergreen broadleaved megaphanerophyte in Taiwan, has straight stems, high tree body and primary leaves with varied color, has a viewing value and is a landscape tree with high development potential. The young stem with axillary buds of Taiwan Cinnamomum kanehirae Hay is used as an explant, the explant is subjected to sterilization, induction culture, multiplication culture, rooting culture, acclimatization, transplanting and the like, so that the Taiwan Cinnamomum kanehirae Hay in-vitro regeneration plant can be acquired; and the Taiwan Cinnamomum kanehirae Hay tissue culture and rapid propagation technology system is established and the technology has important promotion effect on acceleration of the application of good landscape trees in the hinterland.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a technique for the asexual reproduction of Taiwan cinnamomum camphora. Background technique [0002] Cinnamomum camphor ( Cinnamomum kanehirae Hay ), also known as black camphor, is a plant of the genus Lauraceae, native to Taiwan. It is mainly distributed in the natural broad-leaved forest zone at the junction of the subtropical zone and the tropics. Its plant composition is mainly Lauraceae and Fagaceae, and it is called Quercus camphorae community in plant ecology. The trunk is straight, the tree body is tall, and the color of the primary leaves is changeable. It has great ornamental value and is a landscape tree species with great development potential. Its fruit is in the shape of a mitral, and the wood mainly contains terpineol, which has a special fragrance and is not easy to decay. The material is fine, the texture is interlaced, and i...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 朱炳贵
Owner 朱炳贵
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