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Acid treatment agent, sample pretreatment method, kit and detection method for igf-i chemiluminescent immunoassay

A technology of chemiluminescent immunity and detection kits, which is applied in the direction of chemiluminescence/bioluminescence, biological testing, preparation of test samples, etc., which can solve the adverse effects of sample detection accuracy and repeatability, insufficient release of IGF-I, Unable to detect issues such as exact values

Active Publication Date: 2018-05-04
SHENZHEN NEW INDS BIOMEDICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, there are many problems in this method: First, because the samples are pre-diluted before detection, and the result is multiplied by the dilution factor to obtain the final IGF-I concentration, so a small number of low-value samples cannot detect its concentration. exact value, and dilution may adversely affect detection accuracy and reproducibility for some samples
Secondly, incubation was not performed in the sample pretreatment in the above kit, and there may be a risk of insufficient release of IGF-I in some samples

Method used

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  • Acid treatment agent, sample pretreatment method, kit and detection method for igf-i chemiluminescent immunoassay
  • Acid treatment agent, sample pretreatment method, kit and detection method for igf-i chemiluminescent immunoassay

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] A chemiluminescent immunoassay kit for IGF-I, comprising the following components:

[0070] 1) Acidic treatment agent: It is mainly composed of acetic acid solution with a concentration of 0.25mol / L and hydrochloric acid solution with a concentration of 0.05mol / L, according to the molar ratio of acetic acid and hydrochloric acid being 10:1. The value is 2.5.

[0071] 2) Magnetic microsphere system: magnetic microsphere solution coated with anti-IGF-I antibody 1, wherein: the concentration of anti-IGF-I antibody 1 is 50 μg / L, and the concentration of magnetic microspheres is 1.2 mg / ml.

[0072] 3) Marker system: ABEI-labeled anti-IGF I antibody 2 solution, wherein: the concentration of anti-IGF-I antibody 2 is 50 μg / L, and the concentration of ABEI is 0.5 mg / L.

[0073] 5) Calibrator solution: including a low-point calibrator solution with an IGF-I concentration of 63.218 ng / L and a high-point calibrator solution with an IGF-I concentration of 1124.680 ng / L.

[0074] 4...

Embodiment 2

[0086] A method for IGF-I chemiluminescent immunoassay using the detection kit of Example 1, which is basically the same as the detection method in Example 1, except that:

[0087] 1) Pretreatment: Add 10 μL of sample and 50 μL of acid treatment agent into the cuvette, mix well, and incubate at 37° C. for 5 minutes.

Embodiment 3

[0089] A method for IGF-I chemiluminescent immunoassay using the detection kit of Example 1, which is basically the same as the detection method in Example 1, except that:

[0090] 1) Pretreatment: Add 100 μL of sample and 50 μL of acid treatment agent into the cuvette, mix well, and incubate at 37° C. for 5 minutes.

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Abstract

The invention discloses an acid treatment agent for IGF‑I chemiluminescent immunodetection, a sample pretreatment method, a kit and a detection method, and belongs to the technical field of in vitro diagnosis and detection. The acid treatment agent is mainly composed of an organic acid solution with a concentration of 0.1-0.5mol / L and an inorganic acid solution with a concentration of 0.01-0.1mol / L, or an organic acid solution with a concentration of 0.3-2mol / L. The pH value of the acid treatment agent is 1.5-3.5. After the acid treatment agent is mixed with the sample, IGF-I and IGFBP in the sample can be well separated, meeting the detection requirements. Therefore, samples in a low concentration range can be accurately and reliably detected. The IGF-I chemiluminescent immunoassay kit, the sample pretreatment method and the detection method using the above-mentioned acid treatment agent can accurately and reliably detect samples in a low concentration range.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis and detection, in particular to an acid treatment agent for IGF-I (insulin-like growth factor I) chemiluminescent immunodetection, a sample pretreatment method, a kit and a detection method. Background technique [0002] Insulin-like growth factor-I (IGF-I) is a single-chain polypeptide composed of 70 amino acids with endocrine, autocrine and paracrine properties, with a molecular weight of about 7.5KU. play an important regulatory role. Many tissues and organs in the human body express IGF-I gene, and local tissue IGF-I is produced in autocrine or paracrine form. It has structural homology to insulin-like growth factor-II and insulin. Insulin-like growth factor-I in blood circulation is combined with insulin-like growth factor-binding protein-3 (IGFBP-3) and acid-labile subunits, and mainly exists in the form of high molecular triads. [0003] In humans, growth hormone (GH) and nutr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/34G01N21/76G01N33/68G01N33/553
Inventor 饶微孙普付金秋李海容刘望李婷华袁锦云
Owner SHENZHEN NEW INDS BIOMEDICAL ENG