Method for extracting multiple pigments from cape jasmine fruits

Abstract

The invention relates to a method for extracting multiple pigments from cape jasmine fruits, and belongs to the field of biological extraction. In the provided method, a homogenous extraction technology is adopted, a biological enzyme method is used to eliminate the impurities, and key technologies such as a column chromatography separation technology, an elution concentration control technology, and the like are used to extract multiple pigments from cape jasmine fruits. The impurities due to the long-time soaking in the conventional method will not be generated in the provided method, moreover, the production time is shortened, the cost is reduced, the labor and materials are saved, and the method is suitable for enterprises.

Description

technical field

[0001] The invention relates to a method for extracting various pigments from gardenia fruit, belonging to the field of biological extraction. Background technique

[0002] Gardenia is the dry and mature fruit of Gardenia jasminoides Ellis (Gardenia jasminoides Ellis), a commonly used clinical traditional Chinese medicine. It is an important bulk of traditional Chinese medicinal materials in China, and is rich in Fujian, Zhejiang, Jiangxi, Hui, Hunan and other provinces. Gardenia yellow pigment is the only natural water-soluble carotenoid pigment extracted from gardenia fruit, which is widely used in medicine, food and cosmetics and other industries. Gardenia blue pigment and gardenia green pigment are natural food colorings made by bioengineering technology. It has been widely used in the coloring of food and cosmetics abroad, but the technology of producing one kind of pigment from gardenia fruit is mature, but there is no good theoretical basis for the t...

Examples

Embodiment 1

[0018] A method for extracting multiple pigments from gardenia fruit, the steps are:

[0019] a) Select gardenia fruits without mold and peel them;

[0020] b) mashing the skinless gardenia fruit, and then washing with ozone water;

[0021] c) Add it into the container containing the extract, pour it in slowly and evenly, or pour it in a large amount without completely sinking to the bottom, and stir it evenly with a stirring device. The extraction time is 3 hours and the temperature is 60°C. Not only stir;

[0022] d) Filter the extract to remove impurities. The filtering method is to divide the original extract into 2 parts, filter separately and then combine the filtrates, and then perform a filter;

[0023] f) Using column layer separation technology, the flow rate of the adsorption column is 60M3 / h;

[0024] g) The alcohol used in the ethanol elution process is 90 degrees, and the prepared gardenia yellow pigment,

[0025] H) Elute with 40-degree etha...

Embodiment 2

[0032] A method for extracting a variety of pigments from gardenia fruit. Select the nephew fruit without mildew, clean and peel it, and crush it with a pulverizer, generally using 80 meshes, and then wash it with ozone water, and put it into an extraction container after washing. In order to make the gardenia fruit powder evenly contact with the extract, two methods are usually used, one is to slowly pour in and soak little by little, of course this is troublesome, the other is to pour in a large amount and use a stirring device Stir for about 10s, then pause for 2-3 minutes to continue pouring and stirring, but add 3-5s to the stirring time after each pouring. Hours, after this step process is finished, impurity removal and filtration are carried out. The impurity removal is usually carried out by biological enzyme method, and then the column layer separation technology is carried out. The flow rate of the adsorption column is 37M3 / h. When the adsorption is completed, the pr...

technical field

[0001] The invention relates to a method for extracting various pigments from gardenia fruit, belonging to the field of biological extraction. Background technique

[0002] Gardenia is the dry and mature fruit of Gardenia jasminoides Ellis (Gardenia jasminoides Ellis), a commonly used clinical traditional Chinese medicine. It is an important bulk of traditional Chinese medicinal materials in China, and is rich in Fujian, Zhejiang, Jiangxi, Hui, Hunan and other provinces. Gardenia yellow pigment is the only natural water-soluble carotenoid pigment extracted from gardenia fruit, which is widely used in medicine, food and cosmetics and other industries. Gardenia blue pigment and gardenia green pigment are natural food colorings made by bioengineering technology. It has been widely used in the coloring of food and cosmetics abroad, but the technology of producing one kind of pigment from gardenia fruit is mature, but there is no good theoretical basis for the t...

Examples

Embodiment 1

[0018] A method for extracting multiple pigments from gardenia fruit, the steps are:

[0019] a) Select gardenia fruits without mold and peel them;

[0020] b) mashing the skinless gardenia fruit, and then washing with ozone water;

[0021] c) Add it into the container containing the extract, pour it in slowly and evenly, or pour it in a large amount without completely sinking to the bottom, and stir it evenly with a stirring device. The extraction time is 3 hours and the temperature is 60°C. Not only stir;

[0022] d) Filter the extract to remove impurities. The filtering method is to divide the original extract into 2 parts, filter separately and then combine the filtrates, and then perform a filter;

[0023] f) Using column layer separation technology, the flow rate of the adsorption column is 60M3 / h;

[0024] g) The alcohol used in the ethanol elution process is 90 degrees, and the prepared gardenia yellow pigment,

[0025] H) Elute with 40-degree etha...

Embodiment 2

[0032] A method for extracting a variety of pigments from gardenia fruit. Select the nephew fruit without mildew, clean and peel it, and crush it with a pulverizer, generally using 80 meshes, and then wash it with ozone water, and put it into an extraction container after washing. In order to make the gardenia fruit powder evenly contact with the extract, two methods are usually used, one is to slowly pour in and soak little by little, of course this is troublesome, the other is to pour in a large amount and use a stirring device Stir for about 10s, then pause for 2-3 minutes to continue pouring and stirring, but add 3-5s to the stirring time after each pouring. Hours, after this step process is finished, impurity removal and filtration are carried out. The impurity removal is usually carried out by biological enzyme method, and then the column layer separation technology is carried out. The flow rate of the adsorption column is 37M3 / h. When the adsorption is completed, the pr...