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Method for screening mutant with or without endurable storage

A screening method and a storage-resistant technology, applied in the field of rice breeding, can solve the problems of poor rice quality and decreased germination rate, and achieve the effects of high mutation rate, low cost and light damage

Inactive Publication Date: 2015-07-01
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0002] During the storage of rice, the rice quality deteriorates and the germination rate decreases, which has always been an urgent problem that plagues rice breeding experts.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] A screening method for storage-intolerant mutants, comprising the following steps:

[0023] (1) Using a 42-month natural aging test, the storage characteristics of 6 rice germplasm materials including Wanjian 2090 (Yunnan), Daw Dam (Thailand) and Chongju (Yunnan) were studied, and it was found that Wanjian 2090 was After 42 months of storage at room temperature, it still maintains a germination rate of 98%, which is a rice variety that is resistant to storage;

[0024] (2) Using N + Ion beam (energy: 25kev; beam current: 2500×2.6×1O 13 N + / cm 2 ) irradiated for 10-20 minutes to process the above-mentioned storage-resistant rice variety seed M0, planted in the field, M1 plants were separated in character, and after single-plant screening from M1 to M3 generations, stable 100-200 single-plant materials with basically the same agronomic traits were obtained. , and then further multiply the single plant material to form a strain, which is its near-isogenic line;

[00...

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Abstract

The invention discloses a method for screening a mutant with or without endurable storage. The method comprises the following steps: treating rice varieties with good or poor storage characteristics by utilizing N+ ion beams for irradiating for 10-20 minutes, expanding propagation for 3-4 seasons, screening 100-200 near-isogenic line materials of which the offspring has the consistent agronomic traits; and screening by utilizing natural aging of 3-5 years or high-temperature high-humidity artificial accelerated aging of 30 days, thereby obtaining the mutant without endurable storage from the near-isogenic line of rice varieties with endurable storage, or obtaining the mutant with endurable storage from the near-isogenic line of rice varieties without endurable storage.

Description

technical field [0001] The invention relates to the field of rice breeding, in particular to a screening method for storage-resistant or storage-intolerant mutants. Background technique [0002] During the storage process of rice, the quality of rice deteriorates and the germination rate decreases, which has always been an urgent problem to be solved for rice breeding experts. The research on storage-resistant rice is a research work of great significance to ensure national food security. Contents of the invention [0003] The purpose of the present invention is to provide a screening method for storage-resistant or storage-intolerant mutants. [0004] The above purpose is achieved through the following schemes: [0005] A screening method for storage-resistant or storage-intolerant mutants, characterized in that: using N+ ion beam irradiation for 10-20 minutes to treat storage-resistant or storage-intolerant rice variety seeds, namely M0, planting in the field, and M1 p...

Claims

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Application Information

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IPC IPC(8): A01H1/04
Inventor 张瑛滕斌吴敬德宣红周俊峰
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI