Method for rapidly identifying black shank resistance of tobaccos

A technology for tobacco black shank disease and tobacco black shank bacteria, which is applied in the field of crop disease resistance identification, can solve the problems of relatively harsh conditions for toxin production of pathogenic bacteria, difficult to determine the concentration of toxin, limited amount of toxin extracted, and the like. The effect of accelerating the screening of disease-resistant germplasm resources, saving manpower and material resources, and shortening the identification time

Inactive Publication Date: 2015-07-01
TOBACCO RES INST HENAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantages: firstly, the extraction process of the whole coarse toxin is cumbersome and time-consuming, which takes about 28 days, and the amount of toxin extracted is limited; secondly, the requirements for the production of toxins by pathogenic bacteria are relatively strict, and the light, temperature, and dark treatment time, There are certain choices of culture medium, etc.; in addition, when the toxin is used to treat seeds or tissues, the concentration of the toxin is not easy to determine. Repeated trials are required to determine the appropriate concentration range, so the workload is relatively large.

Method used

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  • Method for rapidly identifying black shank resistance of tobaccos
  • Method for rapidly identifying black shank resistance of tobaccos

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Effect test

Embodiment 1

[0022] A method for rapidly identifying tobacco black shank resistance, comprising the following steps:

[0023] Step 1, cultivation of tobacco black shank bacteria:

[0024] Tobacco black shank race No. 0; inoculated on oat medium and cultured at 28°C for 7 to 10 days. After the mycelia covered the entire dish, use an inoculation loop to remove the mycelia attached to the surface of the dish and leave the bacteria ready for use;

[0025] Step 2: Disinfect the surface of the tobacco seeds to be tested:

[0026] Take Gexin No. 3 seeds and place them in a sterile petri dish, soak them in 75% alcohol for 3 minutes, then soak them in 10% sodium hypochlorite for 5 minutes, and then wash them repeatedly with sterile water 3 times to reduce the residue of disinfectant;

[0027] Step 3, germination rate, morbidity statistics, and disease resistance evaluation:

[0028] Step 2 sterilized tobacco seeds were transferred under aseptic conditions to the tobacco black shank bacteria dish...

Embodiment 2

[0030] A method for rapidly identifying tobacco black shank resistance, comprising the following steps:

[0031] Step 1, cultivation of tobacco black shank bacteria:

[0032] Tobacco black shank race No. 0; inoculated on oat medium and cultured at 28°C for 7 to 10 days. After the mycelia covered the entire dish, use an inoculation loop to remove the mycelia attached to the surface of the dish and leave the bacteria ready for use;

[0033] Step 2: Disinfect the surface of the tobacco seeds to be tested:

[0034] Take the Jinxing 6007 seeds to be tested and place them in a sterile petri dish, soak them in 75% alcohol for 3 minutes, then soak them in 10% sodium hypochlorite for 5 minutes, and then wash them repeatedly with sterile water 3 times to reduce the residue of disinfectant;

[0035] Step 3, germination rate, morbidity statistics, and disease resistance evaluation:

[0036] Step 2 sterilized tobacco seeds were transferred under aseptic conditions to the tobacco black s...

Embodiment 3

[0038] A method for rapidly identifying tobacco black shank resistance, comprising the following steps:

[0039] Step 1, cultivation of tobacco black shank bacteria:

[0040] Tobacco black shank race No. 0 was inoculated on oat medium and cultured at 28°C for 7 to 10 days. After the mycelia covered the entire dish, the mycelium attached to the surface of the dish was removed with an inoculation loop, and the bacteria remained. ready for use;

[0041] Step 2: Disinfect the surface of the tobacco seeds to be tested:

[0042] Put the safflower dajinyuan seeds to be tested in a sterile petri dish, soak them in 75% alcohol for 3 minutes, then soak them in 10% sodium hypochlorite for 5 minutes, and then wash them repeatedly with sterile water 3 times to reduce the residue of disinfectant ;

[0043] Step 3, germination rate, morbidity statistics, and disease resistance evaluation:

[0044] Step two sterilized tobacco seeds were transferred under aseptic conditions to the tobacco ...

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Abstract

The invention belongs to the field of crop disease resistance identification and especially a method for rapidly identifying the black shank resistance of tobaccos. The method for rapidly identifying the black shank resistance of the tobaccos is capable of effectively overcoming the defects of exiting identification, and comprises the following steps: step 1, culturing tobacco black shank bacteria; step 2, taking tobacco seeds to be tested and sterilizing the surface; and step 3, performing germination rate and morbidity statistics and performing disease resistance evaluation. The method is capable of saving manpower and material resources and also greatly reducing the identification time; the resistance level to the black shank is not limited by the growth seasons; besides, the method is simple in identification operation steps and relatively low in test room requirement conditions; according to the method, the purpose of rapidly identifying the black shank resistance of the tobaccos at low cost and high flux is achieved; the method is suitable for identifying the black shank resistance of a large batch of germplasm materials in tobacco breeding study; and as a result, the anti-disease germplasm resource screening, the resistance hereditary character study and the anti-disease variety breeding and popularization/application can be accelerated.

Description

technical field [0001] The invention belongs to the field of crop disease resistance identification, in particular to a method for quickly identifying tobacco black shank resistance Background technique [0002] Tobacco black shank (Phytophthora parasitica var.nicotianae Tucker) is a devastating soil-borne disease that harms tobacco. It has a wide range of distribution, a short incubation period, rapid onset, and high incidence, and it will occur in combination with other diseases. The annual loss of tobacco leaves due to tobacco black shank in North Carolina, USA, accounts for about 1% to 2% of the total output; the loss caused by black shank in 1955 and 1966 reached 36.1 million US dollars, accounting for 35.13% of the disease loss , ranking first among all diseases. The occurrence of tobacco black shank disease in China began from 1919 to 1945, and after the 1980s, tobacco black shank disease spread rapidly in my country. According to the survey, except for Heilongjiang...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12N1/14A01G7/00
Inventor 丁燕芳李雪君平文丽孙焕孙计平李耀宇李彦平马浩波吴昭辉李旭辉侯咏俎焕新
Owner TOBACCO RES INST HENAN ACADEMY OF AGRI SCI
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