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Problems solved by technology
At present, the main limitation of the application of Virosome lies in the large limitation of antigen loading. There are relatively strict requirements on the molecular size and hydrophilicity and hydrophobicity of the antigen, and the loading efficiency needs to be improved.
Method used
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Embodiment 1
[0060] In this embodiment, the vaccine vector is prepared by the following method, and the specific steps are as follows:
[0061] Disperse 5 g of the artificially cultivated Lactobacillus casei sludge in 100 mL of 0.01 mol / L hydrochloric acid solution to obtain a microbial suspension, and transfer it to a hydrothermal reaction kettle placed in a constant temperature box to maintain a pressure of 3 MPa at 180 ° C. Heating at a constant temperature for 10 hours, washing the obtained precipitate with pure water and freeze-drying to obtain the vaccine carrier (DB).
[0062] Utilize scanning electron microscope (JEOL, JSM-6700F) and transmission electron microscope (JEOL, JEM-1400) to carry out the characterization of the prepared vaccine carrier, as figure 1 As shown, the prepared vaccine vector retained the morphology characteristics of bacilli. In addition, a porous microstructure with an average pore diameter of 27.66 nm was formed on the surface of the carrier by virtue of t...
Embodiment 2
[0064] In this embodiment, the vaccine vector is prepared by the following method, and the specific steps are as follows:
[0065] Disperse 2 g of the artificially cultivated Lactobacillus casei sludge in 100 mL of 0.1 mol / L hydrochloric acid solution to obtain a microbial suspension, and transfer it to a hydrothermal reaction kettle placed in a constant temperature box to maintain a pressure of 2 MPa at 200 ° C. Heating at a constant temperature for 12 hours, washing the obtained precipitate with pure water and freeze-drying to obtain the vaccine carrier.
[0066] The prepared vaccine carrier was characterized by scanning electron microscopy, as shown in figure 2 As shown, due to the hydrothermal reaction in the acidic solvent environment, a large number of macroporous structures are formed on the surface of the carrier material.
Embodiment 3
[0068] In this embodiment, the vaccine vector is prepared by the following method, and the specific steps are as follows:
[0069] Disperse 5 g of the artificially cultivated Lactobacillus casei sludge in 100 mL of 0.01 mol / L hydrochloric acid solution to obtain a microbial suspension, and transfer it to a hydrothermal reaction kettle placed in a constant temperature box to maintain a pressure of 3 MPa at 100 ° C. Heating at a constant temperature for 72 hours, washing the obtained precipitate with pure water and freeze-drying to obtain the vaccine carrier.
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Abstract
The invention provides a vaccine preparation as well as a production method and the use of the vaccine preparation. The vaccine preparation comprises a vaccine carrier and an antigen component, wherein the vaccine carrier is obtained by performing hydrothermal conversion on microorganisms. The vaccine preparation is obtained by compounding the vaccine carrier obtained by performing hydrothermal conversion on microorganisms with the antigen component. According to the vaccine preparation, the vaccine carrier material prepared by virtue of the hydrothermal reaction is optimized in properties such as porosity, hydrophilcity and hydrophobicity and surface immune related ligand density, and therefore, when the vaccine carrier material is compounded with the antigen component, the antigen loading efficiency is improved, the vaccine preparation is enabled to have a strong immune activation effect, the immunogenicity of the loaded antigen is effectively enhanced, and the immunotherapy and prevention of a specific disease are realized by virtue of specific immune response of an organism to the antigen. Besides, the vaccine preparation is wide in raw material source, and has universality in vaccine construction; and as a result, a plurality of vaccine preparations to different diseases can be constructed.
Description
technical field [0001] The invention belongs to the field of vaccine preparations, and in particular relates to a vaccine preparation and its preparation method and application. Background technique [0002] In the history of vaccine development, the first vaccines were made from attenuated or inactivated pathogenic organisms such as bacteria, viruses, and Rickettsia. The vaccine preparations obtained in this way have potential biosafety problems in clinical application, such as causing severe inflammatory reactions and pathogenic virus infections. Modern vaccine preparations usually use purified or recombinant subunit antigens to replace complete pathogenic organisms, which greatly improves the biological safety of vaccines because it eliminates the possibility of pathogenic recurrence of pathogens. However, compared with intact pathogenic microorganisms, subunit antigens in the form of proteins or polypeptides are susceptible to degradation and difficult to be taken up by...
Claims
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