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Cell in vitro culture device and culture method

A culture device and cell body technology, applied in tissue cell/virus culture devices, artificial cell constructs, animal cells, etc., can solve problems such as inability to achieve layered dynamic perfusion culture of two different culture fluids, and maintain cell The effect of form

Inactive Publication Date: 2017-01-25
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of this, it is necessary to provide an in vitro cell culture device and culture method for the problem that the layered dynamic perfusion culture of two different culture solutions cannot be realized in the existing cell culture

Method used

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  • Cell in vitro culture device and culture method
  • Cell in vitro culture device and culture method
  • Cell in vitro culture device and culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Such as figure 1 As shown, an in vitro cell culture device includes a bioreactor 1, a dual culture fluid irrigation system, a loading device and a monitoring system.

[0042] As shown in 2, the bioreactor 1 includes a cover plate 11, a middle plate 12, a bottom plate 13, and a double culture cup 14. The cover plate 11 is placed on the middle plate 12 in a pair, and the bottom plate 13 is placed on the bottom plate. Below the middle plate 12, the middle plate 12 is provided with a middle plate square hole 121, and a double culture cup 14 is placed in the middle plate square hole 121.

[0043] Such as Figure 3-5 As shown, the double culture cup 14 includes a square cup 141 and a round cup 142 located in the square cup 141. The round cup 142 is composed of a round cup wall and a detachable round cup porous bottom sheet, and the round cup porous bottom sheet can allow the culture solution to pass through. The round cup 142 is provided with a round cup suction tube 144 and a ro...

Embodiment 2

[0053] In order to better understand the present invention, the following only takes chondrocytes as an example to illustrate the method of culturing cells using the cell in vitro culture device described in Example 1, including the following steps:

[0054] S1. Prefabricated liquid gel containing chondrocytes; in this embodiment, three-dimensional culture of chondrocytes is used, and any three-dimensional culture carrier in the prior art can be used;

[0055] S2. After assembling the bioreactor and loading device, place it on the ultra-clean table, connect the computer to the motor, and control the motor to adjust the position of the pressure plate so that there is a gap of 1-4 mm between the porous pressure sheet and the round cup porous bottom sheet. Inject the liquid gel prefabricated in step S1 into the gap, make the liquid gel fully contact the surface of the porous pressing sheet and the porous bottom sheet of the round cup, and place it at room temperature until the liquid g...

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Abstract

The invention discloses an in vitro cell culture device. The in vitro cell culture device comprises a bioreactor, a double nutrient solution irrigating system, a loading device and a monitoring system. The invention also discloses a method for culturing the cell by using the in vitro cell culture device. The method comprises the following steps: biologically simulating a physiological status, establishing the double nutrient solution irrigating system, and supplying different nutrients to different tissue blocks; allocating two pipe systems for each double culture cup, taking the culture tissue as a spacer layer of two culture solutions, culturing by simultaneously using two culture solutions, automatically irrigating the culture solutions, timely and quantificationally changing the cell culture solution, regulating the irrigating speed of the culture solution and constantly providing various nutrients and stable pH value for the cell. A dynamic pressurized environment is adopted for the cell culture device and the cell culture method; a pressure is exerted on the culture tissue by exerting a controllable axial compressive deformation load; the pressure and the compressive deformation of each culture tissue can be monitored and analyzed in real time and the pressure can be adjusted.

Description

Technical field [0001] The present invention relates to the field of cell culture in vitro, in particular to a cell culture device and culture method in vitro. Background technique [0002] The proliferation, differentiation, and the realization of physiological functions of cells or tissues are closely related to the environment in which they are located. These environmental factors can be one or more of nutrients, pressure, temperature, pH, etc. There are many tissues and cells in the human body that live in a dynamic load environment, such as chondrocytes and lung cells. These tissues and cells can only proliferate and differentiate normally and produce the same physiological functions as in the body when they are cultured under appropriate pressure conditions. Existing in vitro studies have shown that: articular chondrocytes that are not cultured in vitro under appropriate pressure have irregular cell arrangement, less matrix production, and finer collagen fibers; at the same...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/34C12N5/071C12N5/077
Inventor 邬培慧廖威明傅明
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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