Micro-droplet treatment device and using method thereof

Abstract

The invention relates to a micro-droplet treatment device. The micro-droplet treatment device comprises a sample introduction system, a micro-fluidic chip system, a temperature control system, a liquid drop identification system, a liquid drop detection system and a control system, wherein the sample introduction system is used for introducing a water phase and an oil phase into the micro-droplettreatment device, the sample introduction system at least comprises a sample introduction system I and a sample introduction system II, the sample introduction system I is used for introducing the oilphase into the micro-droplet treatment device, and the sample injection system II is used for introducing the water phase into the micro-droplet treatment device; the micro-fluidic chip system comprises a substrate, a pipeline formed in the substrate, a first detection window and a second detection window; the temperature control system comprises a temperature sensor and a temperature control component; the liquid drop identification system comprises a laser light source and a photoelectric sensor; the liquid drop detection system comprises an optical fiber spectrometer and a halogen light source; and the control system is used for controlling each system in the micro-droplet processing device.

Description

technical field [0001] The invention belongs to the field of microfluidics, and in particular relates to a micro-droplet processing device and a method for using the same. Specifically, the micro-droplet processing device may be a microbial droplet cultivation device. Background technique [0002] Microfluidic chip technology is widely used in sample preparation, reaction, separation, and detection in biological, chemical, and medical analysis processes. It is one of the most rapidly developing cutting-edge technologies and the most active fields of research. The microfluidic chip that processes the sample solution has high requirements on the stability of sterilization and sample injection. The sample injection of the microfluidic chip needs to be slow, such as at the pL-nL / s level. The stability is higher, and slight fluctuations will affect the stability and uniformity of the droplets. [0003] Traditional microbial breeding generally adopts plate spreading culture to ob...

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Problems solved by technology

[0006] Patent document CN 103983794A disclosed a microfluidic chip in 2014, a microfluidic chip, including a detection window (detection hole), an electrode, a channel containing a bifurcated structure, a liquid inlet pool (or liquid inlet interface), Finished product pool (or liquid outlet interface), waste liquid pool (or waste liquid interface), etc., liquid inlet pool (or liquid inlet interface), finished product pool (or liquid outlet interface), waste liquid pool (or waste liquid interface) respectively The channel is physically connected, the detection window (or detection hole) is located near the channel, and the electrodes are fixed on both sides of the channel close to the bifurcation, which structurally solves the problem of the identification of the droplet position in microfluidics, the control of the droplet volume, the liquid Segmentation of a single sample in a droplet, separation of multiple samples in a droplet, and screening of droplet parameters have realized the material carrier of microfluidic technology, but the detection function cannot be realized, and the cultivation of microbial droplets cannot be controlled online. and filter

[0007] Patent document CN 104007091A disclosed a high-throughput detection system based on droplet microfluidic chip in 2014, which mainly includes droplet microfluidic chip system, optical path system, and data acquisition and analysis system; the droplet microfluidic The chip system embeds the microorganisms to be detected to form an independent single-droplet micro-reaction chamber, and transmits the laser-induced fluorescence detection signal of the microbial sample in the single-droplet micro-reaction chamber through the optical path system, and the data acquisition and analysis system collects and obtains it through computer software. The detection and analysis of the signal can realize the efficient screening of target enzymes and metabolites related to the production of different types of industrial microorganisms including Escherichia coli, Corynebacterium glutamicum and Saccharomyces cerevisiae, but cannot realize the online cultivation of microorganisms

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