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Method for accelerating growth of muscodor fengyangensis and adopted pretreated active carbon granules

A technology of activated carbon granules and Fengyang gas-producing mold, applied in the field of microbial fermentation, can solve problems such as slow growth of gas-producing mold

Active Publication Date: 2015-07-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although VOCs have a wide range of antibacterial activities, the growth rate of aerogenes is very slow, and it has become a bottleneck that is difficult to break through in industrial fermentation

Method used

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  • Method for accelerating growth of muscodor fengyangensis and adopted pretreated active carbon granules
  • Method for accelerating growth of muscodor fengyangensis and adopted pretreated active carbon granules
  • Method for accelerating growth of muscodor fengyangensis and adopted pretreated active carbon granules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, preservation and activation culture of bacterial strain (belong to routine technology)

[0042] 1. Normal temperature or low temperature storage of strains

[0043]On the ultra-clean workbench, add no more than 1.5ml of melted PDA medium to a 2ml sterile cryopreservation tube, cover it, and place it at an angle. After solidification, inoculate the preserved strains to the slant medium. After a few days, the mycelium successfully colonizes the slant, and fresh mycelium can be seen growing. Add the paraffin that has been sterilized three times to submerge the mycelium, and cover it. Store at room temperature or in a refrigerator at 10°C. When in use, use a sterile toothpick to pick out the bacterial tissue and inoculate it on a flat PDA medium for activation.

[0044] 2. Cryopreservation of strains

[0045] On the ultra-clean workbench, dig out 4-5 pieces of bacteria from the colony on the PDA, put them in a 2ml sterile cryopreservation tube, add high-pre...

Embodiment 2

[0048] Embodiment 2, the pretreatment of gac

[0049] In order to ensure the high adsorption activity of activated carbon, the activated carbon is pretreated as follows, and the following steps are carried out in sequence:

[0050] 1), heating washing:

[0051] Weigh 100g of Hushi brand granular activated carbon that has just been opened, put it in a 1000ml large beaker, add 500ml of deionized water, boil in an electric heating pot for 30min, discard the waste liquid, and repeat 4 times (i.e., carry out 5 times in total); Activated carbon granules after initial wash;

[0052] 2), ultrasonic cleaning:

[0053] Use clean gloves to squeeze the activated carbon particles after the initial washing, drain the water, transfer it to a 1000ml large beaker, add 800ml deionized water, place it in a 53KHZ ultrasonic cleaner for 30min, discard the waste liquid, and obtain the second washing Activated carbon particles;

[0054] 3) Repeatedly wash the activated carbon particles after the...

Embodiment 3

[0058] Inoculate the Mycobacterium aerogenes ZJLQ024 strain on a flat plate PDA medium from the cryopreservation tube for storing the strain, and use it when the diameter of the colony reaches 6-7 cm. Pour the melted PDA medium into one compartment of a sterile three-compartment petri dish, and wait for the medium to cool and solidify for later use. Take the cultured Mold aerogenes ZJLQ024 colony, use a 5mm hole puncher to punch a hole at the edge of the colony to obtain a 5mm diameter bacterial cake, inoculate the bacterial cake in this grid of the plate culture medium that contains the PDA medium, and another Add 1.0 g of activated carbon after autoclaving (obtained in Example 2) to the grid, and seal the culture dish with double-layer Parafilm sealing film. No active carbon was added, and only M. aerogenes ZJLQ024 was added as the control group (that is, the culture medium of M. aerogenes ZJLQ024 without adding activated carbon was used as the control group), and cultured i...

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Abstract

The invention discloses pretreated active carbon granules for accelerating the growth of muscodor fengyangensis. A preparation method sequentially comprises the following steps: (1) performing heating and washing, namely adding deionized water into active carbon granules, boiling the water, and removing waste liquid to obtain preliminarily-washed active carbon granules; (2) performing ultrasonic cleaning, namely adding the deionized water into the preliminarily-washed active carbon granules, ultrasonically cleaning the preliminarily-washed active carbon granules, and removing the waste liquid to obtain secondarily-washed active carbon granules; (3) repeatedly cleaning the secondarily-washed active carbon granules by using deionized water until visible muddy matters do not exist in washing liquid; (4) draining water off from the active carbon granules obtained in the step (3), and then drying the active carbon granules. Meanwhile, the invention further provides a method for accelerating the growth of the muscodor fengyangensis by utilizing the pretreated active carbon granules. The pretreated active carbon granules are arranged in a growth environment of the muscodor fengyangensis and aim to adsorb VOCs generated in the growth process of the muscodor fengyangensis.

Description

technical field [0001] The invention relates to the field of microbial fermentation, and in particular provides a method for accelerating the growth of Fengyang aerogenes by using activated carbon adsorption. Background technique [0002] Muscodor spp. (now also known as musk mold) is a class of endophytic fungi belonging to the family Cycloceraceae. It was first isolated from cinnamon by Worapong et al. (2001). Its remarkable feature is the ability to produce volatile organic Compounds (volatile organic compounds, referred to as VOCs), these VOCs have a wide range of biological activities, can inhibit or kill many pathogenic fungi, pathogenic bacteria and some insects. Later, people used its unique morphological, physiological, biochemical characteristics and ribosomal RNA sequences to find and identify new endophytic fungi of the genus Aerogenes, and more and more species of Aerogenes were discovered. Because M. aerogenes can produce VOCs with a wide range of antibacteria...

Claims

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Application Information

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IPC IPC(8): B01J20/34B01J20/20C12N1/14C12R1/645
Inventor 冯晓晓张雪林福呈章初龙苏珍珠毛黎娟
Owner ZHEJIANG UNIV