Method for improving protective enzyme activity of grass of water stress lawn by adopting reinforced drought-resistant microbial agent

A technology of microbial inoculum and compound microbial inoculant, applied in the field of turfgrass protection enzyme activity

Inactive Publication Date: 2015-07-22
TIANJIN NORMAL UNIVERSITY
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Problems solved by technology

However, there is no report on the research on the enhancement of microorganisms in the natural environ

Method used

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  • Method for improving protective enzyme activity of grass of water stress lawn by adopting reinforced drought-resistant microbial agent

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Experimental program
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Effect test

Embodiment 1

[0086] Preparation of enhanced drought-resistant microbial inoculum:

[0087] (1) Enrichment of strains

[0088] Weigh 10g of the compost sample and place it in a sterile Erlenmeyer flask, add 100mL of sterile water and oscillate evenly, then take 10mL of the suspension in a Erlenmeyer flask filled with 100mL of enrichment medium, at 28°C, 220r / min Shake culture for 3 days, which is the mixed microbial flora;

[0089] (2) Preparation of enhanced composite microbial agent: expand the screened enhanced drought-tolerant microorganisms in corresponding liquid medium; culture Bacillus cereus and Bacillus lysine at 30°C, 180r / min for 32h, sticky red The yeast was cultured at 28°C and 220r / min for 32 hours, and the wavelength of 600 nm (560 nm for fungi) was used for turbidimetric measurement. The OD value of the bacterial suspension was used as the vertical axis, and the culture time was used as the horizontal axis to draw the growth curve of the microorganism; The strains in the ...

Embodiment 2

[0091] Method for improving water stress turfgrass protective enzyme activity by using enhanced drought-resistant microbial agent:

[0092](1) Experimental materials: Tall fescue ( Festuca arundinacea L.) The seeds are the test materials, the soil for the test is the school garden soil, the physical and chemical properties: pH is 7.44, the salt content is 0.29%, the organic matter content is 4.68%, the total nitrogen content is 0.21%, and the available phosphorus content is 22.03mg·kg -1 , total potassium 45.61 g·kg -1 , bulk density 0.46 g·L -1 , saturated water content 0.58 g·mL -1 ;

[0093] (2) Preparation of enhanced composite microbial agent: expand the screened enhanced drought-tolerant microorganisms in corresponding liquid medium; culture Bacillus cereus and Bacillus lysine at 30°C, 180r / min for 32h, sticky red The yeast was cultured at 28°C and 220r / min for 32 hours, and the wavelength of 600 nm (560 nm for fungi) was used for turbidimetric measurement. The OD va...

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Abstract

The invention discloses a method for improving protective enzyme activity of grass of a water stress lawn by adopting a reinforced drought-resistant microbial agent. The reinforced drought-resistant microbial agent comprises the following components of reinforced bacillus cereuses, reinforced lysine bacillus and reinforced rhodotorula glutinis, with the weight ratio of 1: 1: 1. The drought resistance and the reinforcement of a mixed microbial population are performed by adopting the method of gradually increasing the concentration of PEG6000. The invention further discloses the application of the reinforced drought-resistant microbial agent in the field of improving the protective enzyme activity of festuca arundinacea of the lawn grass under the drought stress. Experiments prove that under the drought stress, the protective enzyme activity of the festuca arundinacea can be obviously improved, so that damage caused by the drought stress to the festuca arundinacea is alleviated, and the improvement of the drought-resistance of the lawn grass is facilitated.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and relates to a method for strengthening urban domestic garbage composting microbial bacterial agents to improve the activity of lawn grass protection enzymes under water stress. Background technique [0002] Microbial agents, especially compound microbial agents composed of a variety of microorganisms, are biotechnology developed in recent years with broad application prospects. Microbial agents can not only repair oil-contaminated soil and degrade organic pollutants in water bodies, but also prevent and control pests and diseases, promote crop production and increase production and income. Some microbial agents can improve the stress resistance of plants. Since the 1970s, European and American Japan and other countries or regions have successfully developed some composite bacterial agents, many of which have begun large-scale production and formed serialized products. Among t...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16A01G7/06C12R1/085C12R1/07C12R1/645
CPCC12N1/20A01G7/00A01G7/06A01G20/00C12N1/16
Inventor 多立安赵树兰高星星
Owner TIANJIN NORMAL UNIVERSITY
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