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Application of five myxobacteria to predation of drug-resistance bacteria and preparation of drug-resistance bacteria suppression drugs

A drug-resistant bacteria and drug-resistant technology, applied in the field of microbiology, can solve problems such as the difficulty of antibiotics and the slow progress of new antibiotic-producing bacteria

Active Publication Date: 2015-07-22
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is increasingly difficult to discover new antibiotics from traditional antibiotic-producing bacteria such as Streptomyces, Bacillus subtilis, Pseudomonas, etc.
At the same time, research on finding new antibiotic-producing bacteria is progressing slowly

Method used

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  • Application of five myxobacteria to predation of drug-resistance bacteria and preparation of drug-resistance bacteria suppression drugs
  • Application of five myxobacteria to predation of drug-resistance bacteria and preparation of drug-resistance bacteria suppression drugs
  • Application of five myxobacteria to predation of drug-resistance bacteria and preparation of drug-resistance bacteria suppression drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Research on the predation effect of myxobacteria on drug-resistant bacteria

[0028]The five strains of myxobacteria in this embodiment are respectively: Myxococcus sp.GIM1.810 (preservation number is GIM1.810), My xococcus sp.GIM1.811 (preservation number is GIM1.811), Corallococcusexiguus GIM1.813 (preservation number is GIM1.813), Myxococcus sp.GIM1.815 (preservation number is GIM1.815) and Corallococcus corallo ides GIM1.816 (preservation number is GIM1.816), the above five strains of myxobacteria are all preserved in Guangdong Microbial Culture Collection Center , its deposit number is shown in the brackets behind the strain name, and the strains in this depository are sold externally, so anyone can buy the strains from this depository.

[0029] The drug-resistant strains used in this example are respectively: three strains of drug-resistant Staphylococcus aureus (S.aureus 11, S.aureus 15, S.aureus 46), four strains of Salmonella (Salmonella sp.31, Salmo...

Embodiment 2

[0042] Example 2. Research on the inhibitory effect of metabolites of myxobacteria on drug-resistant bacteria

[0043] 1. In VY / 2 medium (fresh yeast 5.0g / L, CaCl 2 . 2H 2 01.0g / L, VB120.0005g / L, PH 7.2) were inoculated with five strains of Myxococcus sp.GIM1.810, Myxococcus sp.GIM1.811, Corallococcus exigus GIM1.813, Myxococcus sp.GIM1.815 and Corallococcus coralloides GIM1 .816, 150rpm, cultured at 30°C for 7d.

[0044] 2. Centrifuge at 4000rpm to collect the bacterial cells and the supernatant of the fermentation broth respectively. The fermentation broth was extracted with an equal volume of ethyl acetate for 12 hours. The cells were soaked in acetone, ultrasonically disrupted, and then extracted with ethyl acetate for 12 hours. After the extract was rotary evaporated, methanol was used to dissolve the extract, and the extracts of the fermentation broth and the broken cell liquid were dissolved to concentrations of 50 mg / mL and 100 mg / mL, respectively.

[0045] 3. In...

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PUM

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Abstract

The invention discloses application of five myxobacteria to predation of drug-resistance bacteria and preparation of drug-resistance bacteria suppression drugs. The five myxobacteria, namely, Myxococcus sp.GIM1.810, Myxococcus sp.GIM1.811, Corallococcus exiguus GIM1.813, Myxococcus sp.GIM1.815 and Corallococcus coralloides GIM1.816, are capable of preying drug-resistance bacteria and generating active natural products which are capable of remarkably suppressing the drug-resistance bacteria; therefore, the five myxobacteria have relatively good practical application value in the aspects of the biological control over the drug-resistance bacteria and the development of antibiotic drugs which are capable of effectively suppressing the drug-resistance bacteria.

Description

Technical field: [0001] The invention belongs to the field of microorganisms, and in particular relates to the application of five strains of myxobacteria in preying on drug-resistant bacteria and in the preparation of drugs for inhibiting drug-resistant bacteria. Background technique: [0002] In recent years, with the widespread use and abuse of old antibiotics, especially broad-spectrum antibiotics, various drug-resistant bacteria are increasing and becoming more complicated. At present, the drug resistance of bacteria has become a major threat to the medical industry and human health. In recent years, there are fewer types of new antibiotics on the market, and the means available to these drug-resistant bacteria are very limited. Their emergence is almost a disaster. It is urgent to find and develop antibiotics that can effectively inhibit drug-resistant bacteria. [0003] Microbial natural products have been an important source of new antibiotics. However, it is incr...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/741A61P31/04C12R1/01
CPCY02A50/30
Inventor 褚福鑫朱红惠李安章
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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