Method for inducing umbilical cord mesenchymal stem cells to be differentiated into dopaminergic neurons

Abstract

The invention discloses a method for inducing umbilical cord mesenchymal stem cells to be differentiated into dopaminergic neurons. According to the method, a two-step combination method of pre-induction and induction is adopted, optimized pre-induction culture solution and induction culture solution are prepared, and a glycoside compound improved in structure is added as an inducer, so that the umbilical cord mesenchymal stem cells are successfully induced to be differentiated into the dopaminergic neurons and the differentiation ratio is relatively high.

Description

technical field

[0001] The invention relates to the field of neurobiology, in particular to a method for inducing differentiation of umbilical cord mesenchymal stem cells into dopaminergic neurons, and the invention also relates to cultured neurons. Background technique

[0002] Since Erices et al. reported in 2000 that umbilical cord blood is rich in hematopoietic stem cells and mesenchymal stem cells, umbilical cord blood mesenchymal stem cells have attracted the attention of researchers due to their multi-directional differentiation potential, immunoregulatory effect and no ethical issues. . Subsequently, some scholars reported that umbilical cord blood mesenchymal stem cells could induce the expression of neuronal markers and glial cell markers in vitro. At present, people have been able to successfully isolate and culture umbilical cord blood mesenchymal stem cells in vitro, and have a further understanding of their biological characteristics, surface markers and immun...