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Paecilomyces lilacinus for prevention and control of meloidogyne diseases of tomato and grape

A technology for Paecilomyces lilacinus and root-knot nematode disease, applied in the field of microorganisms, can solve the problems of differences in control effects of root-knot nematodes, etc., and achieve low nutritional requirements and good growth effects

Active Publication Date: 2015-08-05
宁夏张裕龙谕酒庄有限公司
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Problems solved by technology

[0005] Paecilomyces lilacinus has infectivity to root-knot nematodes, but due to differences in the characteristics of individual organisms, the control effects of Paecilomyces lilacinus on root-knot nematodes from different sources are different, so it is necessary to continuously separate them to have a better effect on root-knot nematodes. highly pathogenic strains

Method used

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  • Paecilomyces lilacinus for prevention and control of meloidogyne diseases of tomato and grape
  • Paecilomyces lilacinus for prevention and control of meloidogyne diseases of tomato and grape

Examples

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Embodiment

[0023] Example 1 : Strain YT08 Separation and screening

[0024] Paecilomyces lilacinus YT08 was isolated from the epidermis of kelp cultured in Yantai City, Shandong Province, China. The separation and screening are carried out according to the following steps: Dilute the collected kelp samples with sterile water in a ratio of 1:100, shake them in a constant temperature shaker at 28°C for 30 minutes, and apply them after being gradually diluted on an ultra-clean workbench. Screen the medium plate on a constant temperature shaker at 28°C for 4-6 days until a single colony grows. Pick a single colony on the separation medium plate for multiple streak separation and purification. After the pure culture is checked by a microscope, it is transferred Connected with red bengal solid slant medium, cultured in a constant temperature shaker at 28°C for 4-6 days and stored at 4°C, it is Paecilomyces lilacinus YT08.

[0025] The components of the screening medium used are as follows: amm...

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Abstract

The present invention discloses a strain of Paecilomyces lilacinus for prevention and control of meloidogyne diseases of tomato and grape. The Paecilomyces lilacinus of the present invention is Paecilomyces lilacinus YT08, has the preservation number of CGMCC No.10026, is preserved in the China General Microbiological Culture Collection Center (CGMCC) in November 26, 2014, provides strong prevention and control effects for meloidogyne of tomato and grape, and can be developed as the novel biological prevention and control agent for the agriculture meloidogyne diseases.

Description

technical field [0001] The present invention relates to a Paecilomyces lilacinus ( Paecilomyces lilacinus ), belonging to the field of microbial technology. Background technique [0002] Plant nematode disease is one of the four major pathogenic organisms in the cultivation of crops, vegetables, fruit trees, forest trees, flowers and medicinal materials. Its harm is second only to fungi and surpasses bacteria and viruses. Plant parasitic nematodes cause about 100 billion US dollars of losses in world agricultural production every year, and root-knot nematodes are the most harmful type of plant parasitic nematodes. [0003] The control of plant parasitic nematodes mainly includes chemical nematicides, cultivation techniques, breeding resistant varieties and biological pesticides. Because root-knot nematodes usually live in the soil and in the roots of plants, there are still no specific chemical pesticides, and they can only control the occurrence of nematode diseases in a ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01P5/00C12R1/79
CPCA01N63/30C12N1/145C12R2001/79
Inventor 程仕伟刘树朋张萍
Owner 宁夏张裕龙谕酒庄有限公司
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