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Human chorionic gonadotropin immunochromatography detection test paper and preparation method thereof

A detection technology of chorionic gonadotropin and immunochromatography, which is applied in the direction of biological testing, measuring devices, and analysis materials, can solve the problems of single function of immunochromatography detection method, interference of multiple detection lines, and inability to judge results, etc., to achieve Simple operation effect

Inactive Publication Date: 2015-08-05
陈岩松
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a human chorionic gonadotropin immunochromatographic detection test paper and its preparation method, which solves the problem of single function in the existing immunochromatographic detection method, interference between multiple detection lines, inability to The problem of accurate determination of results

Method used

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  • Human chorionic gonadotropin immunochromatography detection test paper and preparation method thereof
  • Human chorionic gonadotropin immunochromatography detection test paper and preparation method thereof
  • Human chorionic gonadotropin immunochromatography detection test paper and preparation method thereof

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Embodiment 1

[0035] The preparation procedure of human chorionic gonadotropin immunochromatographic test paper is as follows:

[0036] Step 1. Preparation of reaction membrane 2

[0037] preparation figure 2 The reaction membrane shown in . Prepare the α-HCG monoclonal antibody solution according to the attached figure 2 The figure in the figure is coated on the nitrocellulose membrane, and the detection points T1~T6 are coated with different contents of α-HCG monoclonal antibody, and the antibody content in T1 point is 100 μg / cm 2 , the antibody content in T2 spot is 40μg / cm 2 , the antibody content in T3 spot is 10μg / cm 2 , the antibody content in T4 spot is 5μg / cm 2 , the antibody content in T5 spot is 0.5μg / cm 2 , the antibody content in T6 spot is 0.1μg / cm 2 , Drying for 24 hours, the drying temperature is 37 ℃, humidity ≦ 40% RH.

[0038] Step 2. Preparation of Conjugate Release Pad 5

[0039] Take 40nm colloidal gold solution, add 0.2M K at a ratio of 1.2% 2 CO 3 (Potas...

Embodiment 2

[0056] The preparation procedure of human chorionic gonadotropin immunochromatographic test paper is as follows:

[0057] Step 1. Preparation of reaction membrane 2

[0058] preparation image 3 The reaction membrane shown in . Prepare the β-HCG monoclonal antibody solution according to the attached image 3 The figure in the figure is coated on the nitrocellulose membrane, the detection points T1~T6 are coated with different contents of α-HCG monoclonal antibody, and the antibody content of T1 point is 5 μg / cm 2 , T2 point antibody content 10μg / cm 2 , T3 point antibody content 40μg / cm 2 , T4 point antibody content 0.5μg / cm 2 , Drying for 24 hours, the drying temperature is 37 ℃, humidity ≦ 40% RH.

[0059] Step 2. Preparation of Conjugate Release Pad 5

[0060] Take 40nm colloidal gold solution, add 0.2M K at a ratio of 1.2% 2 CO 3 (Potassium carbonate) to adjust the pH value, add α-HCG monoclonal antibody at a ratio of 15 μg / ml to make a marker, leave it at room tem...

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Abstract

The invention relates to a human chorionic gonadotropin immunochromatography detection test paper and a preparation method thereof, wherein 2-30 independent detection points are formed at certain intervals in a detection zone on the immunochromatography detection test paper. At least two detection points are arranged at a certain interval in a direction being vertical to the moving direction of a to-be-test sample liquid, and a monoclonal antibody of the human chorionic gonadotropin in different contents is coated by at least two detection points. The human chorionic gonadotropin immunochromatography detection test paper is simple and quick in operation, can achieve qualitative and semi-quantitative detection of the targeted substance, human chorionic gonadotropin, in the to-be-test sample, can be used for assistantly monitoring whether a fetus is growing or not during pregnancy step of women, and can be widely applied in the field of pregnancy detection.

Description

technical field [0001] The invention belongs to the field of in vitro diagnostic reagents, and more specifically relates to a human chorionic gonadotropin immunochromatographic detection test paper and a preparation method thereof. Background technique [0002] Immunochromatography is a rapid diagnostic technique that emerged in the 1990s. figure 1 The structure of human chorionic gonadotropin immunochromatographic detection test paper in the prior art is shown, which is used for pregnancy test detection, such as figure 1 As shown, it includes a sample pad 4, a conjugate release pad 5, a reaction membrane 2, an absorption pad 3, and a backing 1. The reaction membrane 2 includes a detection area T and a quality control area C. The principle is to first immobilize specific antibodies on On the reaction membrane 2, when the sample pad 4 of the test paper is immersed in the sample to be tested, due to capillary action, the sample to be tested will move forward along the membran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/76
CPCG01N33/543G01N33/76G01N2333/59
Inventor 陈岩松
Owner 陈岩松