Production method of high protein feed rich in tryptophan

A technology of high-protein feed and production method, which is applied in the field of production of high-protein feed rich in tryptophan, can solve the problem that it is difficult to increase the content of tryptophan in fermented protein feed, achieve simple process, and reduce the probability of contamination by bacteria , The effect of reducing feed cost

Inactive Publication Date: 2015-08-12
河南双成生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Microorganisms such as bacillus, yeast, and lactic acid bacteria can be used to directly ferment agricultural and sideline products such as soybean meal. Although the anti-nutritional factors of soybean meal can be eliminated and the digestibility can be improved, the It is difficult to increase the content of tryptophan in fermented protein feed

Method used

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  • Production method of high protein feed rich in tryptophan
  • Production method of high protein feed rich in tryptophan
  • Production method of high protein feed rich in tryptophan

Examples

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Effect test

Embodiment 1

[0024] The tryptophan-rich high-protein feed production method of the present invention comprises the following steps:

[0025] The first step, liquid fermentation

[0026] The Corynebacterium glutamicum numbered CICC 20192 on the beef extract peptone was made into a suspended bacterial liquid with 200ml sterile saline, and inoculated into the liquid fermentation medium (filling volume 60%), medium composition: glucose 120g / L, (NH 4 ) 2 SO 4 30g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H 2 O 0.5 g / L, biotin 8×10 -5 g / L, CaCO 3 30 g / L; pH7.0, sterilized at 0.07MPa for 8min. Culture conditions: tank pressure 0.1 Mpa, stirring speed 230 r / min, ventilation rate 1:0.25, culture temperature 30°C, fermentation time 48 h; use 200ml sterile Physiological saline is used to make a suspended bacterial liquid, and when the acid production rate of the Corynebacterium glutamicum fermentation liquid reaches above 3.6%, it is enough to insert the suspension of Candida tropicalis and contin...

Embodiment 2

[0038] The tryptophan-rich high-protein feed production method of the present invention comprises the following steps:

[0039] The first step, liquid fermentation:

[0040] The mixed fermentation of Corynebacterium glutamicum and Candida tropicalis is the same as in Example 1.

[0041] The slanted seeds of Bacillus natto with the number CICC23413 were inoculated in a 500ml eggplant-shaped bottle filled with peptone beef extract medium, and the first-grade solid seeds of Bacillus natto were made into a suspended bacterial solution with 200ml sterile physiological saline, and inoculated into Fermentation culture in 2m3 fermenter (seed tank medium filling volume 60%), medium composition: soybean meal 2.0%, corn flour 1.5%, magnesium sulfate 0.4%, manganese sulfate 0.05%, sodium chloride 1.0%. Culture conditions: tank pressure 0.05 Mpa, stirring speed 190 r / min, ventilation rate 1:0.8, culture temperature 35°C, fermentation time 20 h.

[0042] The second step, the preparation...

Embodiment 3

[0052] The tryptophan-rich high-protein feed production method of the present invention comprises the following steps:

[0053] The first step, liquid fermentation:

[0054] The preparation method of the mixed liquid of Corynebacterium glutamicum (CICC 20192) and Candida tropicalis is the same as in Example 1; the production method of Lactobacillus plantarum fermentation mash is the same as that of Example 1; the production method and implementation of Bacillus natto fermentation mash Example 2 is the same;

[0055] The second step, the preparation of mixed fermented mash:

[0056] Get 30 parts of the Corynebacterium glutamicum and Candida utilis mixed bacteria fermentation liquid obtained in the first step, 10 parts of Lactobacillus plantarum seed liquid, 10 parts of Bacillus natto seed liquid and mix evenly, with Ca(OH) 2 Adjust the pH to 7.0 to obtain fermented mash;

[0057] The third step, solid state fermentation:

[0058] According to weight ratio, 150 parts of deh...

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Abstract

The invention discloses a production method of high protein feed rich in tryptophan. The production method includes: subjecting corynebacterium glutamicum, candida tropicalis, lactobacillus plantarum and bacillus natto to liquid fermentation, mixing fermented liquor rich in metabolites and living bacteria, mixing mixture with soybean meal and the like proportionally, performing solid fermentation, and drying and packaging a fermentation product to obtain the high protein feed higher than 50% in crude protein content and higher than 0.9% in tryptophan content. The production method has the advantages that the process is simple, the fermented liquor is directly mixed with solid material for fermentation, the metabolite, tryptophan, of microorganisms is made use, solid fermentation time is greatly shortened, chances of sundry bacteria contaminating in the solid fermentation process of raw materials are slimmed, energy consumption is low, no waste is discharged in the fermentation process, the feed produced has lowered content of anti-nutritional factors in plant meals, the content of the tryptophan is higher than 0.9%, less exogenous tryptophan is added or the tryptophan is replaced, and cost of the feed is reduced.

Description

technical field [0001] The invention relates to feed production, in particular to a method for producing high-protein feed rich in tryptophan. Background technique [0002] Agricultural and sideline products such as soybean meal, cotton meal and peanut meal have a large output in the grain and oil industry, and are generally used as protein raw materials for animal feed, and are widely used in feed processing, livestock and poultry, and aquaculture industries. However, this kind of cake belongs to vegetable protein and contains a large number of anti-nutritional factors, such as trypsin inhibitors, soybean antigenic protein, uric acid, plant lectins, non-starch polysaccharides, etc., which affect the digestion and absorption of feed by animals. Therefore, in production, it is necessary to effectively remove anti-nutritional factors in cakes and improve feed utilization; , a low-cost substitute for high-quality feed protein raw materials. High-quality feed protein raw mater...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K1/00
Inventor 曹胜炎宋安东谢慧杨森李伟李琰
Owner 河南双成生物科技有限公司
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