Establishment method and application of hplc standard fingerprint of Xiaoer Ganmaoning syrup water extract
A standard fingerprint, Ganmaoning syrup technology, which is applied in the field of establishing the HPLC standard fingerprint of Xiaoer Ganmaoning syrup water extract, can solve the problem that the component analysis cannot comprehensively reflect and control the overall quality of the water extract, and achieve the method Simple, reproducible and stable results
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Embodiment 1
[0048] Embodiment 1 The establishment method of HPLC standard fingerprint of Xiaoer Ganmaoning syrup water extract
[0049] (1) Preparation of the test solution: get the following raw materials in parts by weight, Arctium 80, Scutellaria baicalensis 80, Bellflower 67, Peucedanum 80, Angelica dahurica 27, Fried Gardenia 40, Jiao Hawthorn 27, Liushenqu (Jiao) 27, Burnt malt 27, reed root 120, honeysuckle 120, forsythia 80, add water to decoct twice, the first time is 2 hours, the amount of water added is 6 times the total weight of medicinal materials, the second time is 1 hour, the amount of water added is 5 times the total weight of medicinal materials , filter the decoction, combine the filtrates, let stand for 48 hours, take the supernatant, then centrifuge at 4000 rpm for 10 minutes, take the supernatant after centrifugation, and obtain.
[0050] (2) HPLC determination: precision draw 10 batches of need testing solution (being Xiaoermaoningning syrup aqueous extract) prepar...
Embodiment 2
[0065] Embodiment 2 methodological verification
[0066] The HPLC chromatographic conditions determined in Example 1 were verified by methodology, and the precision, repeatability and stability were investigated.
[0067] Accurately weigh 10.25 mg of baicalin and 10.16 mg of chlorogenic acid reference substance and put them in the same measuring bottle, add methanol to make a solution containing 0.04 mg / mL respectively, accurately measure 10 μL, inject it into the liquid chromatograph, and follow the implementation The HPLC assay method of Example 1 continuously injected 6 times, recorded the chromatogram, and calculated the RSD value.
[0068] Table 3 Precision test of reference substance solution
[0069] times
[0070] The precision of baicalin was 0.71%, the precision of chlorogenic acid was 1.56%, both were less than 2.0%, indicating good precision.
[0071] Repeatedly and accurately measure 10 μL of the test solution, inject it into the liquid chromatograph, in...
Embodiment 3
[0080] Example 3 Application of Xiaoer Ganmaoning Syrup Water Extract HPLC Standard Fingerprint
[0081] According to the method of Example 1, the HPLC standard fingerprint of the water extract of Xiaoermaoningning syrup was established. In addition, get 3 batches of Xiaoermaoningning syrup water extract samples prepared by the method of Example 1, numbered 001-003, operate in the same way as described in Example 1 by HPLC, and obtain Xiaoermaoningning syrup water extracts to be tested. For the fingerprint of the test sample, see Figure 4-6 , using the software "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System (version 2004A)" to analyze the HPLC fingerprints of the sample HPLC fingerprints of Xiaoer Ganmao Ning syrup water extract and the HPLC standard fingerprints prepared. According to the similarity, the quality of the water extract sample of Xiaoermaoningning syrup was judged, and the sample was judged as qualified if the similarity was greater ...
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