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Erythrobacter and its application in degrading decabromodiphenyl ether

A decabromodiphenyl ether, erythrobacterial technology, applied in bacteria, microorganism-based methods, microorganisms, etc., can solve problems such as long time

Inactive Publication Date: 2017-12-01
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Anaerobic degradation is mainly the process of reductive debromination, that is, the continuous removal of bromine atoms to produce low-brominated diphenyl ethers, and further degradation, which takes a long time

Method used

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  • Erythrobacter and its application in degrading decabromodiphenyl ether
  • Erythrobacter and its application in degrading decabromodiphenyl ether
  • Erythrobacter and its application in degrading decabromodiphenyl ether

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Experimental program
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Effect test

Embodiment 1

[0022] 1. Isolation and identification of Erythrobacter sp. TAW-CT125

[0023] 1. Enrichment, isolation and purification of Erythrobacter sp. TAW-CT125

[0024] Medium:

[0025] Artificial Seawater Medium (MMC): Each liter of medium contains 24g of NaCl, NH 4 NO 3 1g, KCl 0.7g, KH 2 PO 4 2.0g, Na 2 HPO 4 3.0g, pH 7.4, MgSO 4 7g (added after sterilization).

[0026] Trace element salt solution: every liter of trace element salt solution contains 2mg of CaCl, FeCl 3 ·6H 2 O 50mg, CuSO 4 0.5mg, MnCl 2 ·.H 2 O 0.5mg, ZnSO 4 ·.7H 2 O 10 mg.

[0027] Marine bacteria agar 2216 medium and marine bacteria broth 2216 medium were purchased from BD Medical Instruments (Shanghai) Co., Ltd.

[0028] Decabromodiphenyl ether mother liquor: organic solvent dichloromethane, the concentration is 1g / L.

[0029] The Erythrobacter sp. TAW-CT125 of the present invention is obtained by enriching, separating and purifying the surface sediment collected from the sea near Xiamen.

[00...

Embodiment 2

[0041] Degradation analysis of decabromodiphenyl ether by Erythrobacter sp. TAW-CT125:

[0042] Artificial Seawater Medium (MMC): Each liter of medium contains 24g of NaCl, NH 4 NO 3 1g, KCl 0.7g, KH 2 PO 4 2.0g, Na 2 HPO 4 3.0g, pH 7.4, MgSO 4 7g (added after sterilization).

[0043] Trace element salt solution: every liter of trace element salt solution contains 2mg of CaCl, FeCl 3 ·6H 2 O 50mg, CuSO 4 0.5mg, MnCl 2 ·.H 2 O 0.5mg, ZnSO 4 ·.7H 2 O 10 mg.

[0044] Enrichment medium EM (Enrichment Medium): marine bacteria broth 2216 medium.

[0045] Decabromodiphenyl ether degradation medium: 100mL MMC medium + trace elements.

[0046] Proliferation culture of Erythrobacter sp. TAW-CT125: Under sterile conditions, inoculate the strain into a 1L sterilized Erlenmeyer flask filled with 500mL seawater broth culture (2216), and place it in a constant temperature shaker , 28°C, 160rpm, cultivated for 48h.

[0047] Take the enriched bacterial solution in a 500mL ster...

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Abstract

Erythrobacterium and its application in degrading decabromodiphenyl ether relate to the field of microbial application. Erythrobacter sp. TAW‑CT125, the deposit number is CCTCC NO.M 2015210. Erythrobacter sp. TAW‑CT125 can be used in the degradation of decabromodiphenyl ether. Method for degrading decabromodiphenyl ether: inoculate Erythrobacter sp. TAW‑CT125 in a liquid medium with decabromodiphenyl ether as the sole carbon source, and place it in a shaker for enrichment culture under dark conditions. Erythrobacter sp. (Erythrobacter sp.) TAW‑CT125 was cultured for 8 days in a degradation medium with decabromodiphenyl ether as the only carbon source at pH 7.4, 28 ° C, and the rotation frequency of the shaker was 160 rpm. The degradation rate of brominated diphenyl ethers reached 85%.

Description

technical field [0001] The invention relates to the field of microorganism application, in particular to Erythrobacterium and its application in degrading decabromodiphenyl ether. Background technique [0002] Polybrominated diphenyl ethers (PBDEs) are a class of brominated flame retardants, which are widely used in the fields of electronics, electrical appliances, chemicals, textiles and construction. There are three main industrial products of PBDEs: pentabromodiphenyl ether (Penta-BDE), octabromodiphenyl ether (Octa-BDE), and decabromodiphenyl ether (Deca-BDE). Among them, decabromodiphenyl ether with the highest bromine content is currently the most demanded class in the market. Decabromodiphenyl ether is a non-reactive additive flame retardant. Due to the lack of chemical bonds, it is easily released into the environmental medium during production, transportation, use and waste disposal. Moreover, decabromodiphenyl ether has the characteristics of environmental persis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/02A62D101/28
Inventor 邵宗泽王万鹏蔡庆涛赵苒
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION