Paraffin sectioning method of plant tissue

A plant tissue and paraffin sectioning technology, applied in the field of plants, can solve problems such as concentration, time difference, production failure, numerous procedures and links, etc., to enhance the effect of sectioning and observation, reduce the use of reagents, and improve production efficiency Effect

Active Publication Date: 2015-08-19
CHENGDU UNIV
View PDF4 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Conventional procedures for making paraffin sections include steps such as fixation, dehydration, transparency, wax immersion, embedding, and section staining. There are many procedures and links involved, and the cycle is long.
In addition, the reagents, concentrations, and time used for different tissue materials are different, and problems such as slice bending and tissue fragmentation are prone to occur during the production process, resulting in failure of production
[0004] The reasons for the failure of the production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Paraffin sectioning method of plant tissue
  • Paraffin sectioning method of plant tissue
  • Paraffin sectioning method of plant tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1) Take mature buckwheat seeds, use double-sided blades to adopt a cross-cutting method, and follow the attached Figure 4 Cut at dotted line A or B to remove embryo and endosperm.

[0051]2) Soaking: Soak the buckwheat seed peel in warm water at 65-70°C for 5-10 minutes;

[0052] 3) Water-containing embedding: use filter paper to quickly absorb the water on the surface and inside of the soaked peel, so that the moisture content of the peel is kept between 20% and 40%, and then quickly transfer it, with the cut of the peel facing upward, and slowly immerse it in the container. In a metal embedding bottom mold with paraffin wax at 60-65°C; among them, the bottom mold is pre-filled with paraffin wax, and after being placed on ice for 15-25 seconds, the peel is immersed in it and then set up on the embedding bottom mold for embedding box, add appropriate melted paraffin again;

[0053] 4) Slicing: Slice with a common biological slicer, with a slice thickness of 10-16 μm;...

Embodiment 2

[0058] 1)~5) as described in embodiment 1

[0059] 6) The tissue sections were not stained with safranin and fast green, but were immersed in 50%, 75%, and 85% ethanol in turn for 2 minutes each, then placed in absolute ethanol for 10 minutes, and xylene for 10 minutes;

[0060] 7) As described in Example 1. (The obtained slice effect is as follows figure 2 , d=12μm)

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Thicknessaaaaaaaaaa
Login to view more

Abstract

The invention relates to the field of plants, and especially relates to a paraffin sectioning method of a plant tissue. The method is characterized in that buckwheat husk is immersed in warm water to make the water content of the buckwheat husk being 20-40%, a water-containing tissue paraffin embedding technology is adopted to substitute dehydration, transparentization and paraffin infiltration steps of routine paraffin sectioning, and a dissecting needle is used to strip paraffin instead of paraffin removal in the section flatting process, so the time and the operation of the section process are greatly reduced. Buckwheat husk tissue sections with the thickness of 10-16[mu]m can be obtained through the method, and the method makes the tissues complete and display of all positions clear, and obviously enhances the sectioning and observation effects.

Description

technical field [0001] The invention relates to the field of plants, in particular to a method for paraffin sectioning plant tissues. Background technique [0002] Paraffin section is the most widely used method in conventional histological preparation techniques. It is mainly used to observe the morphology of cells and tissues. After being sealed, the prepared slides can be made into permanent slides and stored for a long time. [0003] Conventional procedures for making paraffin sections include steps such as fixation, dehydration, transparency, wax immersion, embedding, and section staining, involving many procedures and links and a long cycle. Moreover, the reagents, concentrations, and time used by different tissue materials are different, and problems such as slice bending and tissue fragmentation are prone to occur during the production process, resulting in failure of production. [0004] The reasons for the failure of the production can be traced back to many defic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N1/28G01N1/30
Inventor 赵钢向达兵宋超万燕谭茂玲晏林宋月周鑫
Owner CHENGDU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap