Three-color fluorescent microscopic imaging system

Abstract

The invention discloses a three-color fluorescent microscopic imaging system, which comprises a three-color laser beam combination module, a first dichroscope, an objective lens and a three-color fluorescent imaging module; the three-color laser beam combination module is used for combining three monochromatic lights into a beam of three-color laser, and projecting the three-color laser on the first dichroscope; the first dichroscope reflects the laser and transmits fluorescence simultaneously, and is used for reflecting the three-color laser and projecting the three-color laser on the objective lens; the objective lens is used for transmitting the three-color laser and collecting excited mixed fluorescence, and projecting the collected mixed fluorescence on the first dichroscope; the first dichroscope is used for transmitting the mixed fluorescence, and projecting the mixed fluorescence onto the three-color fluorescent imaging module; the three-color fluorescent imaging module is used for separating the mixed fluorescence and imaging. According to the system, by utilizing the modularized three-color laser beam combination module and three-color fluorescent imaging module, three-color fluorescent imaging light paths are combined, adjustment and maintenance are facilitated, finally three-color fluorescent simultaneous imaging is realized, and time difference among images is eliminated.

Description

technical field [0001] The invention belongs to the field of microscopic imaging, and more specifically relates to a three-color fluorescent microscopic imaging system. Background technique [0002] In the structural imaging of biological samples, fluorescent labeling technology can specifically mark the tissue structure of interest. Using fluorescent substances of different colors to mark the sample structure allows biologists to observe different structural information at the same time. Biological samples labeled with multiple colors require corresponding imaging systems to distinguish and image fluorescent signals of different colors. Most of the existing commercial systems adopt point-scanning imaging mode to excite multicolor fluorescence, and use photomultiplier tubes to acquire multi-channel signals. This point imaging method has a slow imaging speed and is not suitable for large-area sample imaging. [0003] If you use the line scanning mode or the area imaging mo...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above defects or improvement needs of the prior art, the present invention provides a three-color fluorescence microscope imaging system, the purpose of which is to design a three-color fluorescence microscopy system that can simultaneously detect three-band fluorescence Simultaneous imaging of fluorescence microscopy imaging system, which solves the problem that the existing fluorescence imaging system cannot perform line imaging or surface imaging of multi-color fluorescence at the same time, and the optical path adjustment is relatively complicated.

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