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Electrochemical luminescence sensor array, and preparation method and application thereof

A sensor array and electrochemical technology, applied in the field of environmental monitoring and analytical chemistry, can solve the problems of rapid detection of multiple structural analogs, inability to fully identify, single target analyte, etc., and achieve easy control and automation, strong use value, simple and convenient operation

Inactive Publication Date: 2015-08-19
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although MIT technology can achieve a more significant recognition of structural similarities, it still cannot achieve complete recognition, and in order to improve the performance of selective recognition, the use conditions of MIT technology are very harsh, which has brought many restrictions to practical applications.
Although there have been many reports and studies on electrochemical sensors / electrochemiluminescence sensors based on molecular imprinting technology, their target analytes are single and cannot be well applied to the detection of complex samples, nor can they solve various Rapid detection of structural analogs

Method used

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  • Electrochemical luminescence sensor array, and preparation method and application thereof
  • Electrochemical luminescence sensor array, and preparation method and application thereof
  • Electrochemical luminescence sensor array, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1.1 Fabrication of electrochemiluminescent sensor arrays, such as figure 2 Shown:

[0034] The first step is to use a square ITO quartz glass electrode with a side length of 6cm as the substrate, divide the conductive layer of the substrate into 8 square electrode units with a side length of 4mm, and lead out wires to form 8 sensing units.

[0035] In the second step, the sensing unit is composed of a synthetic sex hormone sensing unit and two blank sensing units, in which template molecules, methacrylic acid, ethylene glycol dimethacrylate, dimethylformamide and benzoin ether are massaged The molar ratio is 1:4:8:15:1 and mixed evenly to make a reaction solution. Take 10 μL of the reaction solution and drop-coat it on a clean and dry ITO quartz glass electrode unit, and irradiate it with a 500W ultraviolet lamp at a distance of 30cm for 30 minutes to complete the polymerization. Molecularly imprinted sensing membrane, using 30 mL of ethanol-acetic acid-deionized wate...

Embodiment 2

[0045] 2.1 Preparation of electrochemiluminescence sensor array:

[0046] The first step is to use a square ITO quartz glass electrode with a side length of 2 cm as the substrate, divide the conductive layer of the substrate into six square electrode units with a side length of 4 mm, and lead out wires to form six sensing units.

[0047] In the second step, the sensing unit is composed of a synthetic sex hormone sensing unit and two blank sensing units, in which template molecules, methacrylic acid, ethylene glycol dimethacrylate, dimethylformamide and benzoin ether are massaged The molar ratio is 1:5:9:16:1 and mixed evenly to make a reaction solution. Take 15 μL of the reaction solution and drop-coat it on a clean and dry ITO quartz glass electrode unit, and irradiate it with a 550W ultraviolet lamp at a distance of 30cm for 30 minutes to complete the polymerization. Molecularly imprinted sensing membrane, using 30 mL of ethanol-acetic acid-deionized water mixed solvent with...

Embodiment 3

[0057] 3.1 Preparation of electrochemiluminescence sensor array:

[0058] The first step is to use a square ITO quartz glass electrode with a side length of 3 cm as the substrate, divide the conductive layer of the substrate into six square electrode units with a side length of 4 mm, and lead out wires to form six sensing units.

[0059] In the second step, the sensing unit is composed of a synthetic sex hormone sensing unit and two blank sensing units, in which template molecules, methacrylic acid, ethylene glycol dimethacrylate, dimethylformamide and benzoin ether are massaged The molar ratio is 1:3:7:13:1 and mixed evenly to make a reaction solution. Take 12 μL of the reaction solution and drop-coat it on a clean and dry ITO quartz glass electrode unit, and irradiate it with a 450W ultraviolet lamp at a distance of 25cm for 25 minutes to complete the polymerization. Molecularly imprinted sensing membrane, using 30 mL of ethanol-acetic acid-deionized water mixed solvent with...

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Abstract

The present invention relates to an electrochemical luminescence sensor array. The array consists of 4-20 sensor units integrated on a same substrate; the sensing units comprise a synthetic sex hormone sensing unit and two blank sensing units; the synthetic sex hormone sensing unit includes a base, a molecular imprinting membrane modified on the substrate and a conductive wire; the molecular imprinting membrane consists of the following materials: a template molecule, a functional unit, a crosslinking agent, a solvent, and a photo-initiator agent in the molar ratio of 1:3 -5:7-9:13-16:1; and the template molecule is a variety of synthetic sex hormones to be detected. The invention also relates to a preparation method and application of the sensor array to detection of synthetic sex hormone. The electrochemical sensor array achieves simultaneous analysis of multiple targets, reduces the interference of matrix on the test results, and has high detection reliability and easy operation; and the sensor array is easy to assemble and adjust, and can be used in environmental monitoring, food safety and other fields.

Description

technical field [0001] The invention relates to the technical field of environmental monitoring and analytical chemistry, in particular to an electrochemical luminescence sensor array, a preparation method and an application. Background technique [0002] With the development of society, more and more chemical substances enter the environment, and then enter the human body through the food chain or through contact. The normal secretion of triols can lead to infertility, reproductive organ cancer, obesity and other diseases related to the reproductive system. Internationally, these substances are called endocrine disruptors, also known as environmental estrogens. There are many types of endocrine disruptors, mainly including phthalate plasticizers in plastic products, organochlorine pesticides widely used in agriculture, polychlorinated biphenyls and surfactant compounds in chemical industry, natural Natural estrogen-like compounds in animals and plants, artificial synthet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
Inventor 李婷婷王邃
Owner NINGBO UNIV
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