Kit for auxiliary evaluation of lung cancer genetic risks of to-be-tested person
A kit and risk technology are applied in the field of kits to assist in evaluating the genetic risk of lung cancer in a subject, which can solve the problems of poor independence of related genes, linkage disequilibrium, etc., so as to avoid cross infection, reduce morbidity, and detect methods simple effect
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[0054] Example 1
[0055] This embodiment provides a kit for assisting in evaluating the genetic risk of lung cancer in a test subject. The kit includes a PCR reaction system, a PCR product purification system, and a sequencing reaction system.
[0056] Among them, the PCR reaction system includes the following primers:
[0057] (1) Primers for the single nucleotide polymorphism site rs2285947 (G→A) of DNAH11 gene:
[0058] rs2285947 upstream primer: AGTATGATGAGTTTGGAGCA (SEQ ID NO:1);
[0059] rs2285947 downstream primer: GGAAATAAGCCAAACTGAGA (SEQ ID NO: 2);
[0060] (2) A primer for the single nucleotide polymorphism site rs2494938 (G→A) on the LRFN2 gene.
[0061] rs2494938 upstream primer: ACTAAGCCTCAGTCAAGGTTTG (SEQ ID NO: 3);
[0062] Downstream primer of rs2494938: TAGCGAGGCATTTGGAACAG (SEQ ID NO: 4);
[0063] (3) Primers for the single nucleotide polymorphism site rs4488809 (T→C) on the TP63 gene:
[0064] s4488809 upstream primer: GGGAAGGTAGAATATATGAGT (SEQ ID NO: 5);
[0065] rs4488...
Example Embodiment
[0094] Example 2
[0095] The difference between this embodiment and embodiment 1 is that the PCR reaction system includes the following primers,
[0096] (1) Primers for the single nucleotide polymorphism site rs2285947 (G→A) of DNAH11 gene:
[0097] rs2285947 upstream primer: AGTATGATGAGTTTGGAGCA (SEQ ID NO:1);
[0098] rs2285947 downstream primer: GGAAATAAGCCAAACTGAGA (SEQ ID NO: 2);
[0099] (2) A primer for the single nucleotide polymorphism site rs2494938 (G→A) on the LRFN2 gene.
[0100] rs2494938 upstream primer: ACTAAGCCTCAGTCAAGGTTTG (SEQ ID NO: 3);
[0101] Downstream primer of rs2494938: TAGCGAGGCATTTGGAACAG (SEQ ID NO: 4);
[0102] (3) Primers for the single nucleotide polymorphism site rs4488809 (T→C) on the TP63 gene:
[0103] s4488809 upstream primer: GGGAAGGTAGAATATATGAGT (SEQ ID NO: 5);
[0104] Downstream primer of rs4488809: AAGCCCTTCAATATCTG (SEQ ID NO: 6);
[0105] (4) Primers for the single nucleotide polymorphism site rs9387478 (C→A) on the DCBLD1 gene:
[0106] rs938...
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