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Immunofluorescence test strip for rapidly and quantitatively detecting ST2 (growth stimulation expressed gene 2) and NT-proBNP (N-terminal-pro-B-type-Natriuretic Peptide) and preparation method of immunofluorescence test strip

A quantitative detection and immunofluorescence technology, applied in the field of clinical medical diagnosis, can solve problems such as many interference factors, poor repeatability, and unsuitability for rapid clinical diagnosis

Inactive Publication Date: 2015-08-26
RELIA BIOTECH JIANGSU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, the method for detecting ST2 and NT-proNP is mainly enzyme-linked immunosorbent assay (ELISA). ELISA technology has the following disadvantages: high requirements for detection equipment, high cost; many interference factors, poor repeatability; long detection time
Therefore, ELISA detection of ST2 and NT-proBNP is not suitable for rapid clinical diagnosis

Method used

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  • Immunofluorescence test strip for rapidly and quantitatively detecting ST2 (growth stimulation expressed gene 2) and NT-proBNP (N-terminal-pro-B-type-Natriuretic Peptide) and preparation method of immunofluorescence test strip
  • Immunofluorescence test strip for rapidly and quantitatively detecting ST2 (growth stimulation expressed gene 2) and NT-proBNP (N-terminal-pro-B-type-Natriuretic Peptide) and preparation method of immunofluorescence test strip
  • Immunofluorescence test strip for rapidly and quantitatively detecting ST2 (growth stimulation expressed gene 2) and NT-proBNP (N-terminal-pro-B-type-Natriuretic Peptide) and preparation method of immunofluorescence test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Such as figure 1 Shown, a kind of immunofluorescence test strip of rapid quantitative detection ST2, it comprises test strip support piece 4 and the sample gasket 1 that overlaps and sticks successively on the test strip support piece, detection membrane 2 and water-absorbing gasket 3, A coupling gasket 5 is arranged between the sample gasket 1 and the detection membrane 2, a first layer of glass fiber gasket 6-1 is arranged above one end of the coupling gasket 5, and a second layer of glass fiber gasket 6-1 is arranged below one end. The gasket 6-2 may also be provided with only the first layer of glass fiber gasket 6-1 above one end of the coupling gasket 5 or without any gasket, and the detection film 2 is provided with a detection line 7, and the detection line 7 is coated with anti-ST2 monoclonal antibody or polyclonal antibody, the other side of detection line 7 is provided with control line 8, and control line 8 is coated with anti-streptavidin (SAV) antibody, on...

Embodiment 2

[0090] like figure 1 As shown, an immunofluorescence test strip for rapid quantitative detection of NT-proBNP, including a test strip support sheet 4 and a sample gasket 1, a detection film 2 and a water-absorbent pad sequentially lapped and pasted on the test strip support sheet 4 Sheet 3, between the sample gasket 1 and the detection membrane 2 is provided with a coupling gasket 5, a first layer of glass fiber gasket 6-1 is arranged above one end of the coupling gasket 5, and a second layer of glass fiber gasket 6-1 is arranged below one end of the coupling gasket 5. One layer of glass fiber pads 6-2 may also be provided with only the first layer of glass fiber pads 6-1 above one end of the coupling pad 5 or without any pad, and the detection membrane 2 is provided with a detection Line 7, the detection line 7 is coated with anti-NT-proBNP monoclonal antibody or polyclonal antibody, the other side of the detection line 7 is provided with a control line 8, and the control lin...

Embodiment 3

[0111] like figure 2 As shown, an immunofluorescence test strip for rapid quantitative detection of ST2 and NT-proBNP, which includes a test strip support sheet 4 and a sample pad 1 and a detection film 2 that are sequentially lapped and pasted on the test strip support sheet 4 and a water-absorbing pad 3, a coupling pad 5 is provided between the sample pad 1 and the detection membrane 2, a first layer of glass fiber pad 6-1 is provided above one end of the coupling pad 5, and a first layer of glass fiber pad 6-1 is provided below one end of the coupling pad 5. There is a second layer of glass fiber gasket 6-2, and only the first layer of glass fiber gasket 6-1 or no gasket can be provided above one end of the coupler gasket 5. On the detection membrane 2 A detection line 1 (7-1) and a detection line 2 (7-2) are provided, the detection line 1 (7-1) is coated with anti-ST2 monoclonal antibody or polyclonal antibody, and the detection line 2 (7 -2) coated with anti-NT-proBNP m...

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Abstract

The invention discloses an immunofluorescence test strip for rapidly and quantitatively detecting ST2 (growth stimulation expressed gene 2) and NT-proBNP (N-terminal-pro-B-type-Natriuretic Peptide) and a preparation method of the immunofluorescence test strip. The immunofluorescence test strip comprises a support sheet as well as a sample pad, a detection membrane and a water absorption pad which are sequentially overlapped and pasted on the support sheet, wherein a coupling compound pad is arranged between the sample pad and the detection membrane; a detection line is formed on the detection membrane and coated with an anti-ST2 and / or anti-NT-proBNP monoclonal antibody or polyclonal antibody; the ST2 antibody and / or the NT-proBNP antibody are arranged in the same position or in adjacent positions; a control line is formed on the other side of the detection line and coated with an anti-SAV (anti-streptavidin) antibody; the coupling compound pad is coated with ST2 antibody and NT-proBNP antibody coupling compounds with different fluorescent labels. The test strip has the advantages of convenience, rapidness, simplicity in operation, accurate result and the like and is applicable to clinical rapid diagnosis.

Description

technical field [0001] The invention belongs to the field of clinical medical diagnosis, and in particular relates to an immunofluorescence test strip for rapidly and quantitatively detecting soluble growth-stimulating expression gene 2 protein (ST2) and N-terminal brain natriuretic peptide (NT-proBNP) and a preparation method thereof. Background technique [0002] The soluble growth-stimulating expression gene 2 protein (ST2) gene was first obtained in the BALB / c-3T3 cell line by Tominaga et al. in 1989. It is one of the members of the IL-1 (interleukin 1) receptor family. Two protein products are expressed: one with a transmembrane structure, called transmodel ST2 (ST2L), and one that can be secreted outside the cell, called secreted ST2 (sST2). Studies have found that ST2 can be expressed by cardiac fibroblasts and cardiomyocytes, and is a myocardial protein induced by biomechanical stress. The ST2 gene is expressed in mast cell-activated T helper 2 (Th2) macrophages and...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/325
Inventor 刘红剑威廉姆·努特刘丽萍张丹黄凯
Owner RELIA BIOTECH JIANGSU
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