Method for producing virus-free mini-potatoes and potato atomization cultivation box
A production method and mini-potato technology, which is applied in the field of potato atomization incubators, can solve the problems that the production supply cannot meet the seeds used for commercial potato production, the potato field reproduction coefficient is low, and the level of industrial production is low, so as to shorten the field reproduction period , Improving the nutritional environment of the roots and reducing the amount of artificial use
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Embodiment 1
[0046] Embodiment 1: the production method of a kind of potato detoxification minipotato of the present invention, comprises the following steps:
[0047] a. Preparation of nutrient solution:
[0048] Prepare MS nutrient solutions with different concentrations of 1 / 8, 1 / 4, 1 / 2, and 1, and use 1N Na2OH or HCl to adjust the pH value of the nutrient solution to 5.5-5.8;
[0049] b. Equipment installation, system disinfection and cleaning:
[0050] Assemble spray incubators of different sizes and specifications as required, and disinfect and clean the entire culture system;
[0051] c. Colonization of virus-free seedlings:
[0052] The virus-free seedlings are discharged on the annular thread of the paired cylindrical seedling-bearing reels (7), and the other shaft of the cylindrical seedling-bearing reels 7 is gently buckled to clamp the virus-free seedlings, and then clamp the seedlings to remove them. The paired cylindrical seedling-bearing reels 7 of the poisonous seedlings...
Embodiment 2
[0067] Embodiment 2: the production method of a kind of potato detoxification minipotato of the present invention, comprises the following steps:
[0068] a. Preparation of nutrient solution:
[0069] Prepare MS nutrient solutions with different concentrations of 1 / 8, 1 / 4, 1 / 2, and 1, and use 1N Na2OH or HCl to adjust the pH value of the nutrient solution to 5.5-5.8;
[0070] b. Equipment installation, system disinfection and cleaning:
[0071] Assemble spray incubators of different sizes and specifications as required, and disinfect and clean the entire culture system;
[0072] c. Colonization of virus-free seedlings:
[0073] Discharge the virus-free seedlings on the annular threads of the paired cylindrical seedling-bearing reels 7, and gently buckle them with the other shaft of the cylindrical seedling-bearing reels 7 to clamp the virus-free seedlings firmly, and then clamp the virus-free seedlings The paired cylindrical seedling-bearing reels 7 are placed in the 6 slot...
Embodiment 3
[0090] Time: May 5, 2010 - October 31, 2010
[0091] Location: Tianchi Mountain Glass Greenhouse, South China Potato Research Center, Enshi, Hubei (1200 meters above sea level)
[0092] Variety: Ema Potato No. 5 variety (national examination).
[0093] Number of virus-free seedlings: 100 bottles of virus-free seedlings in test tubes, with an average of 12.5 roots / bottle, totaling 1250 plants.
[0094] Cultivation density: 2500 plants / ㎡, produce 500 plants / ㎡ of mini potatoes.
[0095] Nutrient solution concentration:
[0096] 1 / 8MS liquid medium rooting culture;
[0097] 1 / 2MS liquid medium for growth and culture;
[0098] 1MS liquid medium for strong seedling cultivation.
[0099] Generations of segmented reproduction: 5 generations
[0100] Number of virus-free seedlings for propagation: 7496 (4 lost in the last operation)
[0101] Pollution rate: 0
[0102] Labor saving: 85%
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