Wheat scab liquid biocontrol agent and preparation method thereof

A technology for wheat scab and biocontrol fungicides, which is applied in biochemical equipment and methods, botanical equipment and methods, and microorganism-based methods, etc., can solve problems such as loss and production failure, and achieve good control effects.

Inactive Publication Date: 2015-09-16
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In a pandemic year, the production is generally reduced by 10-20%, and in seve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Isolation and screening of bacterial strain LM3403

[0016] Collect wild yak dung that grows in the alpine and pollution-free grassland above 3,000 meters above sea level. The method of gradient dilution and coating separation is used to separate the bacterial strains in the sample by coating. Colonies with obvious morphological differences were picked for streak purification, numbering, and preservation for future use. The antibacterial ability of the tested strains was investigated by filter paper method and plate confrontation method, and the strain with the strongest broad-spectrum antibacterial ability was named LM3403. The width of the inhibition zone of strain LM3403 against Fusarium graminearum was (6.7±1.1) mm.

Embodiment 2

[0017] Embodiment 2: identification of bacterial strain LM3403

[0018] The colony of strain LM3403 on the NA plate is round, opaque and milky white, sticky, and the edges are irregular; the cells are rod-shaped, Gram-positive, spore-shaped, and oval. Aerobic growth: positive, motility: positive, V-P test: positive, catalase test: positive, indole test: positive, starch hydrolysis: positive, casein hydrolysis: positive, gelatin liquefaction: positive, glucose fermentation test: Positive, nitrate reduction: positive, urea utilization: positive, the morphological characteristics, physiological and biochemical characteristics of the strain LM3403 are consistent with the standard strain of Bacillus subtilis. The 16S rDNA sequence of strain LM3403 and Bacillus subtilis strain SPA N1(JQ308575), Bacillus subtilis strain ME2(JQ900623) and Bacillus subtilis The homology of strain BDT3(HQ711982) reached 100%. Comprehensive analysis determined that the strain LM3403 was Bacillu...

Embodiment 3

[0019] Example 3: Extraction and analysis of bacterial strain LM3403 antibacterial lipopeptide

[0020] Centrifuge the fermentation broth of the strain at 4°C and 12000rpm for 15min, collect the sterile supernatant; use 6mol / L HCl to adjust the sterile supernatant to pH2.0, let stand overnight at 4°C, and then centrifuge at 12000r / min at 4°C After 15 minutes, collect the precipitate; add an appropriate amount of methanol to the precipitate, stir on a magnetic stirrer for 30 minutes, put it in a refrigerator at 4°C for extraction for 8 hours, and centrifuge at 12,000 rpm for 15 minutes at 4°C to obtain methanol extract. After concentration under reduced pressure and vacuum freeze-drying, the antibacterial substance was obtained and stored at -20°C. The antibacterial substance after vacuum freeze-drying was taken, dissolved in phosphate buffer solution with pH 7.0, prepared into a certain concentration antibacterial substance solution, and stored at 4°C for later use.

[0021] ...

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PUM

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Abstract

The invention belongs to the fields of microbe and prevention and treatment of agricultural diseases, and discloses a wheat scab liquid biocontrol agent and a preparation method thereof. The biocontrol agent comprises active ingredients of bacillus subtilis and fermented metabolites thereof, biochemical fulvic acid and chitosan oligosaccharide. The Bacillus subtilis LM3403 is preserved in China General Microbiological Culture Collection Center and has preservation number of CGMCC No. 9377. The effective components of the biocontrol inoculant cooperate to control wheat scab through different effects, and has the advantages of better control effect, environment-friendliness, no residue, no nuisance and no resistance.

Description

technical field [0001] The invention belongs to the fields of microorganisms and agricultural disease prevention and control, and in particular relates to a liquid biocontrol agent for wheat scab and a preparation method thereof. Background technique [0002] Wheat head blight is a fungal disease caused by Fusarium graminearum infection. It has a strong pathogenicity and can infect all parts of wheat, mainly damaging the ears; it can occur at all stages of wheat growth, and infection at seedling stage Cause seedling rot, middle and late infection causes stalk rot and ear rot, especially ear rot is the most harmful. In a pandemic year, the production is generally reduced by 10-20%, and in severe cases it reaches 80-90%, or even goes out of production, causing huge losses. Wheat head blight not only causes a decrease in wheat yield, quality, and flour yield, but the metabolites of the bacteria contain mycotoxins such as deoxynivalenol and zearalenone, which can cause human ...

Claims

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Application Information

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IPC IPC(8): A01N63/02A61P3/00C12N1/20C12R1/125
Inventor 陈亮关二旗李瑞静王金水卞科
Owner HENAN UNIVERSITY OF TECHNOLOGY
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