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Application of protein P311 to treatment of skin wounds

A P311 and protein technology, applied in the field of P311 protein in the treatment of skin wounds, can solve the problem of non-specific regulation of myofibroblasts, and achieve the effects of accelerating burn wound healing, preventing scarring, and promoting transdifferentiation.

Active Publication Date: 2015-09-16
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still no drug that specifically regulates myofibroblasts, especially the transdifferentiation of epidermal stem cells to myofibroblasts

Method used

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  • Application of protein P311 to treatment of skin wounds
  • Application of protein P311 to treatment of skin wounds
  • Application of protein P311 to treatment of skin wounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Distribution characteristics of P311 protein and markers of transdifferentiation of epidermal stem cells into myofibroblasts in wounds of burn patients

[0027] Wound specimens were collected from patients within 15 days after thermal burn, routinely fixed in formalin, embedded in paraffin, and serially sectioned at 5 μm. One was used for HE staining, and it was found that the cells in the new epidermis were spindle-shaped and loosely connected with adjacent cells ( figure 1 A). One was used for immunofluorescent staining of keratin 19 and α-smooth muscle actin, and it was found that keratin 19 and α-smooth muscle actin double-positive cells and α-smooth muscle actin single-positive cells appeared in the basal layer of the new epidermis ( figure 1 A). One was used for vimentin immunofluorescence staining, and it was found that there were scattered vimentin single-positive cells in the basal layer of the new epidermis ( figure 1 B). The ectopic appearance o...

Embodiment 2

[0028] Example 2 Effect of P311 on Transdifferentiation of Human and Mouse Epidermal Stem Cells to Myofibroblasts Cultured in Vitro

[0029] Cell culture and processing methods: The epidermal stem cells of 1-day-old mice and 18-24-year-old human foreskin were isolated and cultured by classical two-step digestion method and differential adherence method, and the purity of epidermal stem cells was identified as 95% by CD71 and CD49f flow cytometry %above. The experiment was divided into the P311 high expression group and the empty vector group. When the primary epidermal stem cells reached 70% confluence, they were transfected with the constructed P311 adenovirus expression vector and the Myc empty vector (the two vectors were designed according to SEQ ID No.2, Entrusted Chongqing Jinmai Biotechnology Co., Ltd. to construct and synthesize), observe the transfection ratio and change the medium after 24 hours, and detect various indicators after 72 hours.

[0030] The effect of P31...

Embodiment 3

[0033] Example 3 P311 Knockout Mice Burn Wound Healing Regularity and Transdifferentiation Level of Epidermal Stem Cells to Myofibroblasts

[0034] Establishment of animal models of superficial II° burns in mice: adult male P311 wild-type and knockout mice (a gift from Professor Gregory A Taylor, the Department of Medicine, Duke University, USA, knockout sequence such as SEQ ID No.2), weighing about 20g, Routine skin preparation and hair removal 2 days in advance, intraperitoneal injection of 35mg / kg 1% pentobarbital sodium anesthesia, using YLS-5Q desktop super temperature-controlled scald instrument (purchased from Jinan Yiyan Technology Development Co., Ltd.) to make two scalds on the back of the mouse For scald wounds, the injury conditions are as follows: area of ​​scalded head: 2cm 2 , Perm temperature: 80°C, duration: 3s, applied pressure: 500g, the wound is located on the posterior midline, and the distance between the upper and lower wounds is 1.5cm. HE staining reve...

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Abstract

The invention belongs to the technical field of biomedical treatment and in particular relates to an application of a protein P311 to treatment of skin wounds. The new specific regulatory protein P311 is discovered by regulating myofibroblast used in wound healing. The discovered protein P311 provides a new treatment strategy for promoting wound healing and preventing scar formation, brings good news for patients with skin wounds, such as burn patients, diabetic foot patients and surgical patients, and has extensive application and market prospects.

Description

technical field [0001] The invention belongs to the technical field of biomedical treatment, and in particular relates to the use of P311 protein in treating skin wounds. Background technique [0002] Skin wounds caused by trauma, burns, surgery, etc. are one of the most common clinical problems. How to effectively promote wound healing and avoid scar formation is the key to the treatment of skin wounds. However, due to the unclear mechanism of wound healing and scar formation, the therapeutic effects on wounds and scars are very limited. It is currently believed that myofibroblasts play a key role in normal wound healing and scar formation: the strong contractile force produced by myofibroblasts can promote the rapid closure of wounds, but if the activity of myofibroblasts continues to increase, it will lead to scarring form. Although periwound fibroblasts are one of the sources of myofibroblasts, studies in recent years have shown that epidermal stem cells in the basal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61P17/02
Inventor 李海胜罗高兴吴军贺伟峰姚志慧杨思思谭江琳周峻峄
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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