A method for improving the transdifferentiation of adipose-derived stem cells into salivary acinar-like cells

A fat stem cell and transdifferentiation technology, which is applied in the field of tissue engineering and regenerative medicine, can solve the problems of restricting the research and application of stem cell transdifferentiation into acinar-like cells, and the low efficiency of stem cell transdifferentiation, achieving simple composition and high transdifferentiation efficiency , the effect of promoting transdifferentiation

Active Publication Date: 2018-08-31
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

[0002] Stem cell differentiation is one of the research hotspots in the field of tissue engineering and regenerative medicine. At present, there are very few research reports on the transdifferentiation of adipose-derived stem cells into acinar-like cells. The main problem of stem cell transdifferentiation is the short-term stem cell transdifferentiation efficiency. Very low, only 13%-18%, which limits the extensive research and application of stem cell transdifferentiation into acinar-like cells

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  • A method for improving the transdifferentiation of adipose-derived stem cells into salivary acinar-like cells
  • A method for improving the transdifferentiation of adipose-derived stem cells into salivary acinar-like cells
  • A method for improving the transdifferentiation of adipose-derived stem cells into salivary acinar-like cells

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[0026] The implementation of the present invention will be described in detail below in conjunction with the drawings and examples.

[0027] A method for improving the transdifferentiation of adipose stem cells into salivary gland acinar-like cells of the present invention comprises the following steps:

[0028] 1. Cultivation of salivary gland cells: Under aseptic conditions, bilateral submandibular glands of C3H mice were taken, digested with 0.1% type II collagenase to obtain salivary gland cells, and cultured in vitro. Main components of the medium: DMEM medium, 10% fetal bovine serum, 1% double antibody, 0.1 μg / mL epidermal growth factor, 5.0 μg / mL transferrin, 5.0 μg / mL insulin, 0.4 μg / mL hydrogenation cortisone.

[0029] 2. Cultivation of adipose stem cells: Under aseptic conditions, bilateral inguinal fat of C3H mice was taken, digested with 0.1% type Ⅰ collagenase to obtain adipose stem cells, and cultured in vitro. The culture medium is DMEM medium, 10% fetal bovin...

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Abstract

Salivary gland cells and adipose-derived stem cells are respectively cultured by adopting a digestion method; co-culture is performed on the salivary gland cells and the adipose-derived stem cells with a transwell culture chamber and a 24-well culture plate; the salivary gland cells are inoculated in an inner chamber, and the adipose-derived stem cells are inoculated in an outer chamber; a DMEM (dulbecco's modified eagle medium) containing fetal calf serum of which the volume fraction is 10 percent is adopted as the culture medium; platelet-rich fibrin leach liquor of which the mass fraction is 0.5 to 5 percent is added to induce the transdifferentiation of the adipose-derived stem cells into the salivary gland acinus-like cells. According to a method disclosed by the invention, the efficiency of the transdifferentiation of the adipose-derived stem cells into the salivary gland acinus-like cells can be effectively improved, and seed cells and a new transdifferentiation method are provided for the tissue engineering technology, regenerative medicine research and application.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering and regenerative medicine, and particularly relates to a method for improving the transdifferentiation of adipose stem cells into salivary gland acinar-like cells. Background technique [0002] Stem cell differentiation is one of the research hotspots in the field of tissue engineering and regenerative medicine. At present, there are very few research reports on the transdifferentiation of adipose-derived stem cells into acinar-like cells. The main problem of stem cell transdifferentiation is the short-term stem cell transdifferentiation efficiency. Very low, only 13%-18%, which limits the extensive research and application of stem cell transdifferentiation into acinar-like cells. Therefore, it is necessary to find a high-efficiency stem cell transdifferentiation technology to promote the research and application of stem cell transdifferentiation into acinar-like cells in the regenerati...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775C12N5/071
Inventor 刘斌戴太强许方方徐海燕安然
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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