Cyclodextrin glucosyltransferase mutants with high β-cyclization activity

A technology of glucosyl and cyclization activity, applied in the fields of genetic engineering and enzyme engineering, can solve the problems of poor specificity, low cyclodextrin cyclization activity, and high cost of industrial production of cyclodextrin, and achieves the improvement of β-cyclization activity. Effect
CN104911158BActive Publication Date: 2019-01-11JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2019-01-11

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Abstract

The invention discloses a cyclodextrin glucosyltransferase mutant with high beta-cyclizing activity, and belongs to the field of gene engineering and enzyme engineering. According to the cyclodextrin glucosyltransferase mutant with high beta-cyclizing activity, the 89th tyrosine of beta-CGT enzyme from Bacillus circulans STB01 is mutated into glycine, aspartic acid and asparaginate respectively; double mutant means mutating the 89th tyrosine into the glycine, aspartic acid and asparaginate on the basis that the 577th aspartic acid of the CGT enzyme is mutated into arginine, and the obtained mutant is significantly improved in beta-cyclizing activity than wild CGT enzyme, thus being more applicable to the industrial production of cyclodextrin.
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Description

technical field

[0001] The invention relates to a cyclodextrin glucosyltransferase mutant with high β-cyclization activity, belonging to the fields of genetic engineering and enzyme engineering. Background technique

[0002] Cyclodextrin is a cyclic compound composed of D-glucopyranose connected by α-1,4-glycosidic bonds, among which α-, β- and γ-cyclodextrins composed of 6, 7 and 8 glucose units Essence is most common. Because of its hollow cylindrical structure, it has the characteristics of being hydrophilic on the outside and hydrophobic on the inside. Cyclodextrin can form inclusion complexes with many hydrophobic guest molecules, thereby changing the physical and chemical properties of the guest molecules. Therefore, it is widely used in food, medicine and other industrial fields. has a wide range of applications.

[0003] The industrial production of cyclodextrin adopts enzymatic process, that is, it is synthesized by converting starch through cyclization reaction u...

Claims

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