A kind of copper algae polysaccharide enzymatic hydrolyzate and its application

A copper algae polysaccharide and enzymatic hydrolyzate technology, which is applied in the field of seaweed polysaccharide extract, can solve the problems of ineffectiveness and achieve the effects of mild process conditions, lower intestinal pH, and change the structure of intestinal flora

Active Publication Date: 2018-11-13
HANGZHOU ZHIWEIGUAN FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the development and use of copper algae polysaccharides has become a new research topic, but the current research on copper algae polysaccharides is mainly focused on its moisturizing effect and antioxidant activity, such as the article published by the inventor of the present application [path Separation and purification of copper algae polysaccharide and its application in cutting tobacco moisture by coupling flow-coupled ultrafiltration, Food and Fermentation Industry, 2012, Issue 08, pages 197-202], [Optimization of microwave-assisted extraction of copper algae polysaccharide and its antioxidant activity , Journal of Nuclear Agricultural Sciences, 2014-06, pp. 1062-1069] and patent application CN2015100921966 are all about the antioxidant activity or moisturizing performance of copper algae polysaccharides. Our experiments show that the copper algae prepared according to these documents Enzyme modification of polysaccharides or cuprophyllan has no effect on the improvement of gastrointestinal function

Method used

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  • A kind of copper algae polysaccharide enzymatic hydrolyzate and its application
  • A kind of copper algae polysaccharide enzymatic hydrolyzate and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Enzymatic hydrolysis pretreatment:

[0032] Wash the copper algae, control the water, dry in an oven at 60°C, and pulverize. Choose the copper algae that crosses 100 mesh sieves after the crushing, get 100.0g powder, add in the water of 1500g, adjust pH to be 5.5, add 0.5g compound enzyme, compound enzyme is papain (Shanghai Blue Season Science and Technology Development Co., Ltd., 1,000,000 U / g) mixed with cellulase (Zhangjiagang Jinyuan Biochemical Co., Ltd., 80,000 U / g) in a mass ratio of 1:1, placed in a constant temperature water bath at 50°C, stirred at a constant speed for 60 minutes, centrifuged at 4000rpm for 10 minutes, and taken The clear liquid is the enzymatic hydrolysis pretreatment liquid;

[0033] (2) Alcohol precipitation:

[0034] The enzymatic hydrolysis pretreatment solution was concentrated to 1 L by rotary evaporation, and 95% ethanol solution was added until the final volume fraction of ethanol was 60%, and stood overnight. The sample solu...

Embodiment 2

[0045] The reaction steps and conditions are the same as those in Example 1, the only difference being that in step (4) the reaction was carried out at 50° C. for 3 hours, and finally the enzymatic hydrolysis product SHPA-3 was obtained with an average molecular weight of 10.54 KDa.

Embodiment 3

[0047] Take MRS-glucose medium and 3 MRS sugar-free medium, add SHPA-2, SHPA-3 and FOS (fructo-oligosaccharide) equivalent to glucose respectively in the 3 MRS sugar-free medium (Shanghai Emmons Chemical Technology Co., Ltd.), denoted as MRS-SHPA-2, MRS-SHPA-3, MRS-FOS.

[0048] The fresh feces of the subjects were collected in sterile blue cap bottles and sent for inspection immediately. Quantitatively weigh the specimen, suspend it in ice-cold PBS buffer (pH7.3) to make the fecal sample concentration 50 mg / mL, add sterile glass beads and vortex for 1 min, and let it stand for later use.

[0049] Inoculate the prepared feces suspension into MRS-SHPA-2, MRS-SHPA-3, glucose, and MRS-FOS culture fluid in an amount of 5%, culture on a shaker at 37°C (50rpm), and measure the concentration of the culture fluid after 24h. pH value, see figure 1 . The fermentation broth was serially diluted with PBS buffer solution (pH7.3), and the gradient was set to 10 3 -10 6 Take different g...

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Abstract

The invention discloses an SHP (Sargassum Horneri Polysaccharide) zymolyte and application thereof. The SHP zymolyte is prepared through a method comprising the following steps: (1) performing enzymolysis pretreatment, namely mixing sargassum horneri powder and water, adjusting pH as 5.5, adding a complex enzyme which is a mixture of papain and cellulase according to a mass ratio of 1:1, stirring for 60-80min at the temperature of 50 DEG C, and centrifuging to obtain a supernatant namely enzymolysis pretreatment liquid; (2) performing alcohol precipitation to obtain coarse SHP components; (3) performing radial flow decoloration and deproteinization to obtain SHPA polysaccharide; (4) performing SHPA enzymolysis and purification to obtain an enzymatic hydrolysate; (5) performing ion column chromatography; (6) performing gel column chromatography to obtain the SHP zymolyte. The SHP zymolyte can be used for preparing food or medicines the alteration of intestinal flora. By controlling the molecular weight and the impurity content of the SHP zymolyte, the SHP zymolyte has a remarkable multiplication effect on probiotics, and the intestinal pH is reduced, so that the structure of the intestinal flora can be changed.

Description

(1) Technical field [0001] The invention relates to the technical field of seaweed polysaccharide extracts, in particular to a copper algal polysaccharide enzymatic hydrolyzate and its application in preparing food or medicine for regulating intestinal flora imbalance. (2) Background technology [0002] Intestinal health is one of many health problems that have attracted much attention in recent years. Due to environmental pollution, the deposition of antibiotics in the food chain, changes in the dietary structure of residents, and the improvement of people's health needs, people's attention to health continues to grow. The understanding of health has begun to shift from "outside" and "inside", and more attention is paid to the origin of health-the micro-ecological environment in the body. Prebiotics have great advantages in increasing the number of intestinal endogenous probiotics and regulating the composition of intestinal flora. As a representative prebiotic, polysaccha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14A23L33/00A61K31/715A61P1/00
Inventor 邵平朱岳琼孙培龙
Owner HANGZHOU ZHIWEIGUAN FOOD CO LTD
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