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Immunochromotographic analysis kit, immunochromotographic analysis device, and immunochromotographic analysis method

A technology of immunochromatography and analytical reagents, which is applied in measuring devices, analytical materials, biological tests, etc., can solve the problems of worsening complexity and inability to easily obtain HbA1c, and achieve the effect of inhibiting the reduction of reactivity

Inactive Publication Date: 2015-09-23
TANAKA PRECIOUS METAL IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the measurement of HbA1c has been carried out by HPLC method, capillary electrophoresis method, enzymatic method, immunological measurement method, etc., but because these methods require expertise for a specific device or analysis, there is a problem that in small-scale The value of HbA1c cannot be easily obtained in hospitals or homes, etc.
[0007] In addition, regarding the blood concentration of HbA1c, since there are individual differences in blood components, it is possible to determine whether diabetes is negative or negative by simultaneously measuring hemoglobin other than HbA1c and grasping the ratio of the amount of HbA1c to the total hemoglobin amount of hemoglobin other than HbA1c and HbA1c. positive, but in this case, there is still the problem that HbA1c and hemoglobins other than HbA1c need to be measured separately by the above method, so the complexity of the measurement is further aggravated

Method used

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  • Immunochromotographic analysis kit, immunochromotographic analysis device, and immunochromotographic analysis method
  • Immunochromotographic analysis kit, immunochromotographic analysis device, and immunochromotographic analysis method
  • Immunochromotographic analysis kit, immunochromotographic analysis device, and immunochromotographic analysis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0130] Make it in the following order figure 1 (a) and figure 1 (b) The immunochromatographic analysis device 1 shown.

[0131] (1) Sample addition unit 12

[0132] The sample adding part 12 is produced in this way: on the glass fiber pad (manufactured by Millipore Corporation, trade name: glass fiber conjugate pad (conjugate pad), the size is 32 mm in the vertical direction (the sample processing liquid development direction), 150 mm in the horizontal direction, and the thickness is 0.43 mm). 60μg / cm 2 Add sodium dodecyl sulfate (SDS) evenly, and dry at 50°C for 4 hours.

[0133] (2) Preparation of the anti-HbA1c antibody-coated part 16, the anti-hemoglobin antibody-coated part 17, and the anti-IgG antibody-coated part 18

[0134] As the membrane, a sheet made of nitrocellulose (manufactured by Millipore Corporation, trade name: HF120, 250 mm×25 mm) was used. Dilute the anti-HbA1c monoclonal antibody, anti-hemoglobin monoclonal antibody or anti-IgG monoclonal antibody to...

Embodiment 2

[0162] In addition to changing the alkyl glucoside in the sample diluent in Example 1 from C8 glucoside to C12 glucoside n-dodecyl-β-D-glucoside (produced by Kao Corporation, product name: Mai Doll 12), repeat Example 1. The results are in Table 1 and figure 2 shown in .

Embodiment 3

[0164] In addition to changing the alkyl glucoside in the sample diluent in Example 1 from C8 glucoside to C10 glucoside n-decyl-β-D-glucoside (manufactured by Kao Corporation, product name: Maidor 10 ) except that Example 1 was repeated. The results are in Table 1 and figure 2 shown in .

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Abstract

The purpose of the present invention is to provide an immunochromotographic analysis kit with which high-sensitivity detection can be achieved, and immunochromotographic analysis carried out without causing pain or stress, even in instances in which a blood sample includes saliva taken from within the oral cavity. The problem is solved by an immunochromotographic analysis kit that contains an alkyl glucoside and an anionic surfactant, situated upstream in the development direction from the sample diluent and / or the detection unit. The invention also provides and immunochromotographic analysis device and an immunochromotographic analysis method.

Description

technical field [0001] The invention relates to an immunochromatography analysis kit, an immunochromatography analysis device and an immunochromatography analysis method. Background technique [0002] Measurement of various components contained in samples such as blood and urine is extremely important clinically in understanding the health status of patients, and various measurement methods have been used conventionally for these components. As one of them, an immunochromatography analyzer and an analysis method are known which color a test substance contained in a sample by an immune reaction and confirm the color signal. [0003] The simplest structure of an immunochromatography analyzer is a structure in which a sample adding part, a marker holding part, a chromatography medium carrying a detecting part, and an absorbing part are connected to each other. [0004] On the other hand, the number of diabetic patients in the world was 366 million in 2011, and it is predicted ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558
CPCG01N33/54388G01N33/48G01N33/53G01N33/543G01N33/72G01N33/723
Inventor 冈本浩治加藤佑弥伊藤大辅宫田亚纪中岛晓
Owner TANAKA PRECIOUS METAL IND