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Method and system for extracting and separating dihydro-phaseic acid from desmodium styracifolium

A technology for separating dihydro safflower and quinceum chinensis, which is applied in the field of phytochemistry and achieves the effects of short production time, simple process operation and high purity

Active Publication Date: 2015-09-30
WUHAN OPTICS VALLEY HUMANWELL BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing methods and systems for extracting and separating dihydrosafflower acid need to be improved urgently

Method used

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  • Method and system for extracting and separating dihydro-phaseic acid from desmodium styracifolium
  • Method and system for extracting and separating dihydro-phaseic acid from desmodium styracifolium
  • Method and system for extracting and separating dihydro-phaseic acid from desmodium styracifolium

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Experimental program
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Embodiment 1

[0031] Figure 5 Show the general flow process of the inventive method, comprise following:

[0032] 1. Raw materials: select 45000-50000 parts by weight of the leguminous plant Desmodium glabrata, cut into 5-10cm sections, wash off the sediment with drinking water, drain and feed.

[0033] 2. Ethanol extraction: reflux extraction with 80% ethanol twice, the first time 12 times the amount for 2 hours, the second time 10 times the amount for 1.5 hours; combine the two alcohol extracts, and recover the ethanol until it has no alcohol smell.

[0034] 3. Concentration under reduced pressure: add water to dilute the extract concentrate to 5 times the volume of the medicinal material; filter it with a 200-mesh filter cloth; get the column liquid; pack the net product resin that has been treated with 95% ethanol in advance, and replace it with purified water; The upper column liquid is pumped into the column for adsorption, and the flow rate is controlled to be 0.5-1 times BV / h. Aft...

Embodiment 2

[0045] 1. Carry out steps 1-3 of Example 1 to obtain Guangjin bulk drug.

[0046] 2. Tertiary chromatographic separation

[0047] (1) 300-400 parts by weight of Guangjin crude drug is separated by AB-8 macroporous resin chromatography, that is, chromatographic separation I, ethanol-water mixed solvent 25:75, 35:65, 45:55, 65:35 gradient washing Take off, wash each gradient until there is no color, combine the fractions to get 25%, 35%, 45%, and 65% in four parts.

[0048] (2) The 25% part obtained by chromatography I is separated by ODS reverse-phase medium-pressure chromatography, that is, chromatographic separation II, using ethanol-water, the condition is 15% isocratic for 300 minutes, and 15-35% gradient is eluted for 200 minutes, according to the chromatogram. Peak sequence, collected in fractions, one portion per 100-150 volume fractions, a total of 40-50 fractions were collected, and the same fractions were combined after HPLC detection; 30-40 fractions obtained from c...

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Abstract

The invention provides a method for extracting and separating dihydro-phaseic acid from desmodium styracifolium. The method comprises the following steps that reflux extraction is performed on the desmodium styracifolium by utilizing ethyl alcohol to obtain a desmodium styracifolium extractive; multi-level chromatographic separation is performed on the extractive by utilizing alcohol systems, wherein first-level chromatographic separation is performed on the extractive by utilizing the first alcohol system to obtain a first separation part, second-level chromatographic separation is performed on the first separation part by utilizing the second alcohol system to obtain a second separation part, and third-level chromatographic separation is performed on the second separation part by utilizing the third alcohol system to obtain the dihydro-phaseic acid. The invention further provides a system for implementing the method. The dihydro-phaseic acid is separated and extracted from the desmodium styracifolium by utilizing the method and / or the system, the technology is easy to operate, the production time is short, the production yield is high, the purity is high, and the dihydro-phaseic acid with the purity more than 98 percent can be obtained.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry. Specifically, the invention relates to a method and a system for extracting and separating dihydrosafflower phaseuric acid from Desmodium sativa. Background technique [0002] Desmodium styracifolium (Osbeck) Merr. is a plant of the leguminous genus Desmodium styracifolium (Osbeck) Merr. Its medicinal part is the dry aerial part, and its main chemical components are flavonoids, saponins, polysaccharides, alkaloids and other compounds. It has the effects of clearing away heat and dehumidification, diuresis and treating stranguria. It is used for hot shower, sand shower, stone shower, astringent and painful urination, edema and oliguria, jaundice, red urine, and urinary calculi. [0003] Dihydrosafflower acid is white powder; Molecular formula: C 15 h 22 o 5 ;Molecular weight: 282; figure 1 Show its chemical structure. Dihydrosafflower bean acid exists in Desmodium sativa, which is use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/00C07H17/07C07H1/08C07D407/04
CPCC07D307/00C07D407/04C07H1/08C07H17/07
Inventor 王学海李莉娥许勇杨仲文冯芸余通杨婷尹海龙黄璐曹儒宾谢长
Owner WUHAN OPTICS VALLEY HUMANWELL BIO PHARMA