A small particle size hemoglobin/hydrotalcite-like nanocomposite and its preparation method and application
A nanocomposite and hemoglobin technology, which is applied in the field of small particle size hemoglobin/hydrotalcite-like nanocomposites, can solve the problems of complex operation and cumbersome steps, and achieve the effect of low preparation cost, simple operation and mild conditions
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Embodiment 1
[0024] Weigh MgCl respectively 2 ·6H 2 O and AlCl 3 9H 2 O, add deionized water to prepare MgCl with a total metal ion concentration of 6mmol / L 2 and AlCl 3 Mix the salt solution, add hemoglobin to it to make the concentration 0.2g / L, and record it as solution A after mixing evenly; prepare a sodium hydroxide solution with a concentration of 0.02mol / L, and record it as solution B; open the T-shaped microreactor, and put Add solution A and solution B to the salt solution pipe and lye pipe of the T-type microreactor respectively, control the flow rate, keep the pH of the reaction mixture at about 9.0, and receive the reaction product at the outlet of the mixture; 2 Under protective conditions, stir at low temperature for 24 hours. After the end, centrifuge the mixture at 12,000 rpm for 10 minutes, and wash the precipitate with ultrapure water for 3 times to obtain hemoglobin / hydrotalcite-like nanocomposite jelly, which is designated as Hb-LDH T6 .
Embodiment 2
[0026] Weigh MgCl respectively 2 ·6H 2 O and AlCl 3 9H 2 O, add deionized water to prepare MgCl with a total metal ion concentration of 12mmol / L 2 and AlCl 3 Mix the salt solution, add hemoglobin to it to make the concentration 0.2g / L, and record it as solution A after mixing evenly; prepare a sodium hydroxide solution with a concentration of 0.02mol / L, and record it as solution B; open the T-shaped microreactor, and put Add solution A and solution B to the salt solution pipe and lye pipe of the T-type microreactor respectively, control the flow rate, keep the pH of the reaction mixture at about 9.0, and receive the reaction product at the outlet of the mixture; 2 Under protective conditions, stir at low temperature for 24 hours. After the end, centrifuge the mixture at 12,000 rpm for 10 minutes, and wash the precipitate with ultrapure water for 3 times to obtain hemoglobin / hydrotalcite-like nanocomposite jelly, which is designated as Hb-LDH T12 .
Embodiment 3
[0028] Weigh MgCl respectively 2 ·6H 2 O and AlCl 3 9H 2 O, add deionized water to prepare MgCl with a total metal ion concentration of 24mmol / L 2 and AlCl 3 Mix the salt solution, add hemoglobin to it to make the concentration 0.2g / L, and record it as solution A after mixing evenly; prepare a sodium hydroxide solution with a concentration of 0.02mol / L, and record it as solution B; open the T-shaped microreactor, and put Add solution A and solution B to the salt solution pipe and lye pipe of the T-type microreactor respectively, control the flow rate, keep the pH of the reaction mixture at about 9.0, and receive the reaction product at the outlet of the mixture; 2 Under protective conditions, stir at low temperature for 24 hours. After the end, centrifuge the mixture at 12,000 rpm for 10 minutes, and wash the precipitate with ultrapure water for 3 times to obtain hemoglobin / hydrotalcite-like nanocomposite jelly, which is designated as Hb-LDH T24 .
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