A small particle size hemoglobin/hydrotalcite-like nanocomposite and its preparation method and application

A nanocomposite and hemoglobin technology, which is applied in the field of small particle size hemoglobin/hydrotalcite-like nanocomposites, can solve the problems of complex operation and cumbersome steps, and achieve the effect of low preparation cost, simple operation and mild conditions

Active Publication Date: 2018-04-10
滁州市南方黑芝麻食品有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods either add new raw materials, or the steps are cumbersome and the operation is complicated, and there is still a long way from the requirements of green chemistry.
However, there have been no reports on the preparation of small particle size protein / hydrotalcite-like nanocomposites and their biocatalysis using redox proteins as biomolecular templates by co-precipitation in T-shaped microreactors.

Method used

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  • A small particle size hemoglobin/hydrotalcite-like nanocomposite and its preparation method and application
  • A small particle size hemoglobin/hydrotalcite-like nanocomposite and its preparation method and application
  • A small particle size hemoglobin/hydrotalcite-like nanocomposite and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Weigh MgCl respectively 2 ·6H 2 O and AlCl 3 9H 2 O, add deionized water to prepare MgCl with a total metal ion concentration of 6mmol / L 2 and AlCl 3 Mix the salt solution, add hemoglobin to it to make the concentration 0.2g / L, and record it as solution A after mixing evenly; prepare a sodium hydroxide solution with a concentration of 0.02mol / L, and record it as solution B; open the T-shaped microreactor, and put Add solution A and solution B to the salt solution pipe and lye pipe of the T-type microreactor respectively, control the flow rate, keep the pH of the reaction mixture at about 9.0, and receive the reaction product at the outlet of the mixture; 2 Under protective conditions, stir at low temperature for 24 hours. After the end, centrifuge the mixture at 12,000 rpm for 10 minutes, and wash the precipitate with ultrapure water for 3 times to obtain hemoglobin / hydrotalcite-like nanocomposite jelly, which is designated as Hb-LDH T6 .

Embodiment 2

[0026] Weigh MgCl respectively 2 ·6H 2 O and AlCl 3 9H 2 O, add deionized water to prepare MgCl with a total metal ion concentration of 12mmol / L 2 and AlCl 3 Mix the salt solution, add hemoglobin to it to make the concentration 0.2g / L, and record it as solution A after mixing evenly; prepare a sodium hydroxide solution with a concentration of 0.02mol / L, and record it as solution B; open the T-shaped microreactor, and put Add solution A and solution B to the salt solution pipe and lye pipe of the T-type microreactor respectively, control the flow rate, keep the pH of the reaction mixture at about 9.0, and receive the reaction product at the outlet of the mixture; 2 Under protective conditions, stir at low temperature for 24 hours. After the end, centrifuge the mixture at 12,000 rpm for 10 minutes, and wash the precipitate with ultrapure water for 3 times to obtain hemoglobin / hydrotalcite-like nanocomposite jelly, which is designated as Hb-LDH T12 .

Embodiment 3

[0028] Weigh MgCl respectively 2 ·6H 2 O and AlCl 3 9H 2 O, add deionized water to prepare MgCl with a total metal ion concentration of 24mmol / L 2 and AlCl 3 Mix the salt solution, add hemoglobin to it to make the concentration 0.2g / L, and record it as solution A after mixing evenly; prepare a sodium hydroxide solution with a concentration of 0.02mol / L, and record it as solution B; open the T-shaped microreactor, and put Add solution A and solution B to the salt solution pipe and lye pipe of the T-type microreactor respectively, control the flow rate, keep the pH of the reaction mixture at about 9.0, and receive the reaction product at the outlet of the mixture; 2 Under protective conditions, stir at low temperature for 24 hours. After the end, centrifuge the mixture at 12,000 rpm for 10 minutes, and wash the precipitate with ultrapure water for 3 times to obtain hemoglobin / hydrotalcite-like nanocomposite jelly, which is designated as Hb-LDH T24 .

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Abstract

In the present invention, hemoglobin is used as a biomolecular template, a mixed solution of divalent metal salt Mg(NO3)2 and trivalent metal salt Al(NO3)3 is used as a source of metal ions, and sodium hydroxide solution is used as an alkali source to induce Next, a small-sized hemoglobin / hydrotalcite-like nanocomposite was prepared by co-precipitation in a T-shaped microreactor. In the nanocomposite of the present invention, the hemoglobin molecule is evenly adsorbed on the surface of the hydrotalcite-like particles, and maintains the original secondary structure of its protein; its appearance is spongy and amorphous small particles, with large specific surface area; the average particle size is less than 40nm, and the thickness is less than 5nm; the Zeta potential is between +16mV and +30mV; the hemoglobin / hydrotalcite-like nanocomposite has high thermal stability and can catalyze adjacent The enzyme activity retention rate of phenylenediamine is between 31% and 63.5%, which is much higher than 7.28% of free hemoglobin and 21.44% of hemoglobin complex immobilized by large particle hydrotalcite.

Description

Technical field: [0001] The invention relates to a small-diameter hemoglobin / hydrotalcite-like nanocomposite; the invention also relates to a preparation method of the nanocomposite and its application in catalyzing o-phenylenediamine. Background technique: [0002] The assembly of proteins and inorganic nanoparticles has a wide range of basic and applied research values ​​in the fields of biocatalysis, biomedical diagnosis, and biosensors. The assembly of bio-inorganic interfaces involves interactions such as van der Waals forces, hydrophobic-affinity interactions, electrostatic interactions, and hydrogen bonds. Although inorganic nanoparticles immobilize proteins to improve their performance, the immobilized proteins may be denatured and inactivated due to some unsuitable interface assembly conditions. In order to keep the immobilized protein's original secondary structure and biological activity, it is particularly important to find inorganic nanomaterials with good bioc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K17/14B01J31/38
Inventor 詹天荣李尧杨琦赵继宽
Owner 滁州市南方黑芝麻食品有限公司
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