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Method for preparing litchi polysaccharide fraction with gut immunoregulatory activity under activity tracing

An immunoregulatory activity and lychee polysaccharide technology, which is applied in the field of preparation of intestinal immunoregulatory activity lychee polysaccharide fractions under active tracer, can solve the problems of low solubility of lychee polysaccharides and low intestinal immunoregulatory activity, and achieve high solubility and Effects of intestinal immunomodulatory activity

Active Publication Date: 2015-09-30
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the purpose of the present invention is to overcome the deficiencies in the prior art, provide a kind of preparation method of intestinal immunomodulatory active litchi polysaccharide fraction under activity tracer, solve the solubility and intestinal immune regulation of litchi polysaccharide prepared by common method low activity problem

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0030] A preparation method of litchi polysaccharide fraction with intestinal immunomodulatory activity under activity tracer, comprising the following preparation steps:

[0031] S1. Dried lychee pulp: wash and drain the lychee, send it to hot air drying equipment for 5 hours at 90°C, and then send the lychee to the heat pump drying device, and dry it into the lychee at a temperature of 58°C and a wind speed of 1m / s The moisture content is 25%. During the drying period of the heat pump drying device, it is closed after every 10 hours of drying, and then slowed for 3 hours, and this drying-slow cycle is repeated 3 times;

[0032] S2. Ultra-high pressure enzymatic hydrolysis assisted hot water extraction method to extract litchi crude polysaccharides: add distilled water to dried litchi pulp according to the ratio of material to liquid 1:15, soak at room temperature for 1 hour, beat to obtain a homogeneous solution, and stir at 800 rpm for 25 minutes. Add cellulase 2000U / kg and...

Embodiment 2

[0037] A preparation method of litchi polysaccharide fraction with intestinal immunomodulatory activity under activity tracer, comprising the following preparation steps:

[0038] S1. Dried lychee pulp: wash and drain the lychee, send it to hot air drying equipment for 5 hours at 90°C, and then send the lychee to the heat pump drying device, and dry it into the lychee at a temperature of 58°C and a wind speed of 1m / s The moisture content is 25%. During the drying period of the heat pump drying device, it is closed after every 10 hours of drying, and then slowed for 3 hours, and this drying-slow cycle is repeated 3 times;

[0039] S2. Ultra-high pressure enzymatic hydrolysis assisted hot water extraction method to extract litchi crude polysaccharides: add distilled water to the dried litchi pulp according to the ratio of material to liquid 1:20, soak at room temperature for 2 hours, beat to obtain a homogeneous solution, and stir at 1000rpm for 35min. Add cellulase 3000U / kg and...

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Abstract

The invention discloses a method for preparing a litchi polysaccharide fraction with gut immunoregulatory activity under activity tracing. According to the method, dried litchi pulp is subjected to ultrahigh-pressure enzymolysis assisted hot water extraction for extraction of litchi crude polysaccharide, deproteinization treatment, dialysis for micromolecule desorption and ethyl alcohol fractional precipitation in sequence to obtain the litchi polysaccharide fraction with gut immunoregulatory activity. The litchi polysaccharide prepared with the method has high solubleness and high gut immunoregulatory activity.

Description

technical field [0001] The invention relates to the field of functional foods, in particular to a preparation method of litchi polysaccharide fraction with intestinal immune regulation activity under activity tracer. Background technique [0002] Litchi is the main characteristic fruit in subtropical regions. Because of its bright and beautiful peel, delicate and sweet gravy, it is deeply loved by consumers at home and abroad, and is known as "the treasure of fruit". my country is the main producer of lychees, and its planting area and output both rank first in the world. As the main producing area of ​​lychees, Guangdong Province accounts for more than 60% of the total lychee production in the country. Due to the concentrated time of lychees on the market and the short preservation time, the fresh sales market is under great pressure. It is the only way to promote the sustainable development of litchi industry to carry out research on deep processing and utilization and ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 张瑞芬张名位遆慧慧刘磊邓媛元郭锦欣马永轩魏振承唐小俊
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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