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Chromium-rich aspergillus oryzae as well as production method and application thereof

A chromic-rich Aspergillus oryzae and a production method are applied in the directions of microorganism-based methods, biochemical equipment and methods, applications, etc., to achieve the effects of simple production method, low viscosity and fast growth

Active Publication Date: 2015-09-30
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aspergillus oryzae is often used in the fermentation of condiments such as soy sauce, and there are rare reports on the use of Aspergillus oryzae to prepare organic chromium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Inoculate the spores from the preserved slant of Aspergillus oryzae on the tiger red agar plate, culture at 30-32°C for 48 hours, take 1cm*1cm bacterial block and inoculate it into a 500mL Erlenmeyer flask containing 100mL seed medium, and inoculate at 32°C , Shaking culture at 120r / min for 36 hours to obtain seed solution. The weight percentage of the seed medium consists of: 1% sucrose, 0.6% yeast extract, CrCl 3 ·6H 2 O 0.05%, the balance is water, and the pH is natural.

[0025] Inoculate 100mL of seed solution into a 5L mechanically stirred fermenter with 3L of fermentation medium, at 30-32°C, 200r / min, with an air flow of 3L / min, and cultivate for 48 hours. The weight percentage of the fermentation medium is composed of: sucrose 2 %, yeast extract 1.0%, mannan oligosaccharides 0.01%, CrCl 3 .6H 2 O 0.08%, the balance is water, the pH is natural, collect the culture, filter the filter cake (that is, the bacteria), wash it twice with deionized water, dry it at 6...

Embodiment 2

[0027] Pick spores from the preserved slant of Aspergillus oryzae with an inoculation loop and inoculate them on Tiger Red Agar, and cultivate them at 30-32°C for 56 hours. Take 1 cm * 1 cm bacterial block and inoculate it into a 500 mL Erlenmeyer flask filled with 100 mL of seed medium, and culture at 30-32 °C with shaking at 150 r / min for 48 hours. The composition of the seed medium is: 3% sucrose, 1.2% yeast extract, CrCl 3 .6H 2 O 0.05%, the balance is water, and the pH is natural.

[0028] 100mL seed liquid was inoculated in a 5L mechanically agitated fermenter equipped with 4L fermentation medium (fermentation medium composition: 3% sucrose, 1.2% yeast extract, 0.015% mannan oligosaccharide, CrCl 3 ·6H 2 O 0.1%, the balance is water, pH is natural), 30~32°C, 250r / min, ventilation rate 3.5L / min, cultivated for 56 hours, collected the bacteria, dried and crushed, the finished product is obtained, the chromium content in the finished product was 2.82%.

Embodiment 3

[0030] Use an inoculation loop to pick spores from the preserved slant of Aspergillus oryzae and inoculate them on Tiger Red Agar, and culture them at 30-32°C for 64 hours. Get 1 cm * 1 cm bacterial block and inoculate it into 150 mL seed liquid medium (composed of 2% sucrose, 0.9% yeast extract, CrCl 3 ·6H 2 O 0.05%, the balance is water, pH natural) in a 500mL Erlenmeyer flask, 30 ~ 32 ℃, 130r / min shaking culture for 40 hours.

[0031] 150mL seed solution was inoculated in a 5L mechanically stirred fermenter with 3.5L fermentation medium (fermentation medium composition: 4% sucrose, 1.5% yeast extract, 0.02% mannan oligosaccharide, CrCl 3 ·6H 2 O 0.12%, the balance is water, pH is natural), 30~32°C, 350r / min, ventilation rate 4.0L / min, cultivated for 50 hours, collected the bacteria, dried and crushed, the finished product is obtained, the chromium content in the finished product is 2.95%.

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Abstract

The invention discloses chromium-rich aspergillus oryzae as well as a production method and application thereof. The production method comprises the following steps: getting a spore on an aspergillus oryzae preservation slant, and inoculating the spore onto rose bengal agar for activation; getting a 1 cm*1 cm activated fungus block for inoculation into a triangular flask filled with a seed culture medium, and preparing a seed liquid; getting the seed liquid which accounts for 1-4% of the volume of a fermentation culture medium for inoculation into a mechanical stirring fermentation tank filled with the fermentation culture medium, and carrying out fermentation; collecting fermented products for filtration, and getting a filter cake for drying and crushing, thereby obtaining the chromium-rich aspergillus oryzae. The produced thallus is rich in chromium, that is, each kilogram of the thallus contains 26-30 grams of chromium; the production method is simple and efficient; no fungus seed needs to be domesticated, and the fed-batch culture of inorganic chromium ions is not needed, so that the operation is simplified.

Description

technical field [0001] The invention relates to a kind of aspergillus oryzae, specifically a chromium-rich aspergillus oryzae, and also relates to a production method for producing chromium-rich thallus by culturing aspergillus oryzae and the application of the chromium-rich thallus. Background technique [0002] Chromium is an essential trace element in animals and an important part of glucose tolerance factor. Chromium has the function of regulating fat, sugar and protein metabolism, and can improve the meat quality of poultry and livestock. Inorganic chromium is highly toxic to animals and difficult to absorb; organic chromium is absorbed quickly and is safe. As a new type of feed additive, organic chromium has received extensive attention and application. Commonly used forms of organic chromium include amino acid chelated chromium, cell chromium, etc. Among them, the chromium-enriched cells produced by microbial culture have become a research hotspot for organic chromiu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23K1/18A23K1/16C12R1/69
Inventor 杨柳吴俊贤刘国庆邓杰勇
Owner HEFEI UNIV OF TECH
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