Decellularized heart valve for controllably releasing vascular endothelial growth factor and method of preparation and application thereof
A decellularized valve and growth factor technology, applied in the field of new decellularized valves, biomedicine and biomaterials, can solve the problems of uncontrollable infiltration of plasma components, immaturity, and the speed and effect of endothelialization to be improved, so as to delay calcification and Activation of platelets, effect of preventing exposure
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0086] In a preferred embodiment, the preparation method of the decellularized valve of the present invention comprises the following steps:
[0087] (1) Synthesis of MAL-PEG-PCL
[0088] Using the ring-opening polymerization method, weigh a certain amount of dried MAL-PEG and ε-CL respectively and place them in a dry 25ml three-neck round bottom flask. Using stannous octoate as the catalyst and toluene as the reaction solvent, vacuum repeatedly and fill with nitrogen. 5 times, the reaction was carried out under nitrogen atmosphere. MAL-PEG-PCL was synthesized by ring-opening polymerization under 68°C oil bath heating and magnetic stirring. After 72 hours of reaction, turn off the power of the oil bath, and turn off the nitrogen after the reaction system is cooled to room temperature to obtain the crude product of MAL-PEG-PCL. Rotary evaporation under reduced pressure at 68°C for 2 hours to remove the remaining toluene in the product, after cooling to room temperature, add a...
Embodiment 1
[0155] To prepare a decellularized valve with controlled release of vascular endothelial growth factor, the steps are as follows:
[0156] 1. Synthesis and characterization of maleimide-polyethylene glycol-polycaprolactone (MAL-PEG-PCL)
[0157] 1.1 Synthesis of maleimide-polyethylene glycol-polycaprolactone (MAL-PEG-PCL), the steps are as follows:
[0158] For the synthetic route of MAL-PEG-PCL see figure 1 . Using the ring-opening polymerization method, weigh 1g of MAL-PEG and 0.4mlε-CL that have been dried and place them in a dry 25ml three-neck round bottom flask, add 20 microliters of stannous octoate, dissolve them in 10ml of toluene, and repeatedly evacuate and fill After 5 times of nitrogen gas, the above reactants were heated in a nitrogen atmosphere at 68°C under magnetic stirring in an oil bath to undergo a ring-opening polymerization reaction. After 72 hours of reaction, turn off the power supply of the oil bath, and turn off the nitrogen gas when the reaction sy...
Embodiment 2
[0228] To prepare a decellularized valve with controlled release of vascular endothelial growth factor, the steps are as follows:
[0229] 1. Synthesis of maleimide-polyethylene glycol-polycaprolactone (MAL-PEG-PCL), the steps are as follows:
[0230] For the synthetic route of MAL-PEG-PCL see figure 1 . Using the ring-opening polymerization method, weigh 1g of MAL-PEG and 0.8mlε-CL after drying and place them in a dry 25ml three-neck round bottom flask, add 20 microliters of stannous octoate, dissolve them in 10ml of toluene, and repeatedly vacuum-fill After 5 times of nitrogen gas, the above reactants were heated in a nitrogen atmosphere at 68°C under magnetic stirring in an oil bath to undergo a ring-opening polymerization reaction. After 72 hours of reaction, turn off the power supply of the oil bath, and turn off the nitrogen gas when the reaction system was cooled to room temperature. , to obtain the crude product of MAL-PEG-PCL. The crude product was rotary evaporate...
PUM
Property | Measurement | Unit |
---|---|---|
particle size | aaaaa | aaaaa |
particle diameter | aaaaa | aaaaa |
particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com