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Decellularized valve with controllable release of vascular endothelial growth factor, method of making and application thereof

A decellularized valve and growth factor technology, applied in the field of decellularized valves with controlled release of vascular endothelial growth factor, can solve the problems of immaturity, inability to control the infiltration of plasma components, and the speed and effect of endothelialization to be improved, so as to prevent exposure , Delay the effect of calcification and platelet activation

Active Publication Date: 2019-02-22
THE SECOND AFFILIATED HOSPITAL TO NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem solved by the present invention is: if the surface of the decellularized matrix is ​​not completely covered by endothelial cells, the infiltration of plasma components cannot be controlled, which may cause calcification of the valve tissue and activation of platelets, but the endothelialization technology of the decellularized matrix is ​​not yet available mature, its endothelialization speed and effect need to be improved, and the relevant biological properties of valve stent materials need to be improved

Method used

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  • Decellularized valve with controllable release of vascular endothelial growth factor, method of making and application thereof
  • Decellularized valve with controllable release of vascular endothelial growth factor, method of making and application thereof
  • Decellularized valve with controllable release of vascular endothelial growth factor, method of making and application thereof

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preparation example Construction

[0086] In a preferred embodiment, the preparation method of the decellularized valve of the present invention comprises the following steps:

[0087] (1) Synthesis of MAL-PEG-PCL

[0088] Using the ring-opening polymerization method, weigh a certain amount of dried MAL-PEG and ε-CL respectively and place them in a dry 25ml three-neck round bottom flask. Using stannous octoate as the catalyst and toluene as the reaction solvent, vacuum repeatedly and fill with nitrogen. 5 times, the reaction was carried out under nitrogen atmosphere. MAL-PEG-PCL was synthesized by ring-opening polymerization under 68°C oil bath heating and magnetic stirring. After 72 hours of reaction, turn off the power of the oil bath, and turn off the nitrogen after the reaction system is cooled to room temperature to obtain the crude product of MAL-PEG-PCL. Rotary evaporation under reduced pressure at 68°C for 2 hours to remove the remaining toluene in the product, after cooling to room temperature, add a...

Embodiment 1

[0155] To prepare a decellularized valve with controlled release of vascular endothelial growth factor, the steps are as follows:

[0156] 1. Synthesis and characterization of maleimide-polyethylene glycol-polycaprolactone (MAL-PEG-PCL)

[0157] 1.1 Synthesis of maleimide-polyethylene glycol-polycaprolactone (MAL-PEG-PCL), the steps are as follows:

[0158] For the synthetic route of MAL-PEG-PCL see figure 1 . Using the ring-opening polymerization method, weigh the dried 1g MAL-PEG and 0.4mlε-CL respectively and place them in a dry 25ml three-necked round-bottomed flask, add 20 microliters of stannous octoate, dissolve in 10ml toluene, and repeatedly vacuum-fill After 5 times of nitrogen gas, the above reactants were heated in a nitrogen atmosphere at 68°C under magnetic stirring in an oil bath to undergo a ring-opening polymerization reaction. After 72 hours of reaction, turn off the power supply of the oil bath, and turn off the nitrogen gas when the reaction system was co...

Embodiment 2

[0228] To prepare a decellularized valve with controlled release of vascular endothelial growth factor, the steps are as follows:

[0229] 1. Synthesis of maleimide-polyethylene glycol-polycaprolactone (MAL-PEG-PCL), the steps are as follows:

[0230] For the synthetic route of MAL-PEG-PCL see figure 1 . Using the ring-opening polymerization method, weigh the dried 1g MAL-PEG and 0.8mlε-CL respectively and place them in a dry 25ml three-neck round bottom flask, add 20 microliters of stannous octoate, dissolve in 10ml of toluene, and repeatedly vacuum-fill After 5 times of nitrogen gas, the above reactants were heated in a nitrogen atmosphere at 68°C under magnetic stirring in an oil bath to undergo a ring-opening polymerization reaction. After 72 hours of reaction, turn off the power supply of the oil bath, and turn off the nitrogen gas when the reaction system was cooled to room temperature. , to obtain the crude product of MAL-PEG-PCL. The crude product was rotary evapora...

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Abstract

The invention relates to the fields of biological medicine and biomaterial, and more specifically relates to a decellularized heart valve which can controllably releasing vascular endothelial growth factor and a method of preparation and application thereof. The decellularized heart valve contains sulfhydrylated decellularized heart valve and a nano material which is connected to the sulfhydrylated decellularized heart valve and which can release vascular endothelial growth factor. The nano material is made of raw materials containing the following components: maleimide-polyethylene glycol-polycaprolactone, polycaprolactone and vascular endothelial growth factor; the vascular endothelial growth factor contains VEGF165. The decellularized heart valve provided by the invention has the characteristics that the exogenous biological signal vascular endothelial growth factor is introduced to the decellularized valve support material, thereby accelerating endothelialization of the decellularized valve, and improving the biological performance of the valve support material and the application prospect is wide.

Description

technical field [0001] The invention relates to the fields of biomedicine and biomaterials, mainly relates to the technical field of novel decellularized valves, and specifically relates to a decellularized valve with controllable release of vascular endothelial growth factor and its preparation method and application. Background technique [0002] Heart valve disease is a worldwide public problem that seriously endangers human health, with an incidence rate of about 2.5%. Heart valve replacement is an effective way to treat valvular diseases. By 2050, it is estimated that more than 850,000 patients will need valve replacement surgery. Most of the heart valve replacements commonly used in clinical practice are mechanical valves and biological valves, but they all have serious defects. The disorder of the blood flow field of the mechanical valve often leads to the destruction of the formed components of the blood, and bleeding and thrombosis complications related to the coag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K38/18A61K47/34A61P9/00A61L27/18A61L27/22
Inventor 周建良朱志刚聂彬恩丁静丽陈佳徐建军易应萍董啸唐燕华杨威
Owner THE SECOND AFFILIATED HOSPITAL TO NANCHANG UNIV
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