Photoelectric integrated potential sensor for detecting extracellular biochemical parameters and its preparation method

Abstract

The invention discloses a photoelectric integrated potential sensor for detecting extracellular biochemical parameters and a preparation method thereof. The extracellular biochemical parameters include three parameters, the extracellular acidification rate, the extracellular glucose consumption rate, and the extracellular redox potential generated in the process of cell metabolism. The sensor is a photoelectric composite device with a silicon-based structure. The front side of the chip has a plurality of sensitive areas for the detection of H+ ions, glucose and redox potential. Insulating layer deposited on the surface for isolation. Among them, SiO2 was used as the sensitive material for H+ ions, the electropolymerized polypyrrole / glucose oxidase composite membrane on the metal film was used as the sensitive material for glucose, and the metal film was used as the sensitive material for redox potential. The invention can realize high-sensitivity and real-time detection of multiple extracellular biochemical parameters, and each sensitive area does not interfere with each other, which can provide more comprehensive information for the research on the physiological metabolism of cells.

Description

technical field [0001] The invention relates to the technical field of sensors for analyzing cell physiological metabolism, in particular to a photoelectric integrated potential sensor for detecting extracellular biochemical parameters and a preparation method thereof. Background technique [0002] Living cells respond to external stimuli such as drugs, changes in environmental factors, and receptors, which will cause functional changes such as cell metabolism, which are manifested as changes in biochemical parameters. During catabolism, living cells will absorb metabolic substances (carbon sources such as sugars, amino acids, fatty acids), generate energy (ATP) and release acidic products. Studies have confirmed that the acidification rate (ie H + Concentration change rate) is closely related to the physiological state of cells. The extracellular redox potential (that is, the redox state of the extracellular microenvironment) reflects the activity of free electrons and is...

AI Technical Summary

Problems solved by technology

In addition, mainstream integrated sensors only physically integrate each detection unit, the functions of each unit are independent of each other, the preparation process is complicated, and the volume is usually large, which makes it difficult to achieve detection at the cell level

In addition, the limitations of some detection technologies in terms of safety and miniaturization limit their application in cell detection, such as the detection of extracellular glucose concentration. The commonly used technology is to use an amperometric glucose sensor, which requires a certain The voltage will cause inevitable damage to the cells. Although the traditional potentiometric sensor can avoid the application of voltage, its sensitivity and resolution cannot meet the measurement requirements at the cell level; redox potential detection usually uses traditional large electrodes, and its The detection sensitivity is low, and the volume is large and difficult to package

[0005] Multi-parameter joint detection is beneficial to reflect the growth and metabolism of cells and the response to external stimuli from multiple angles. At present, there is no simultaneous detection of energy consumption, metabolites and free electron activity of cells in the same test chamber. technology

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