Young plant sample critical point drying method for scanning electron microscope

Abstract

The invention discloses a young plant sample critical point drying method for a scanning electron microscope. The critical point drying method comprises the following steps: 1) respectively covering the top and the bottom of a sample basket of a critical point dryer by filter paper, wherein each piece of filter paper is provided with a plurality of holes for facilitating exchange between isoamyl acetate and CO2, and placing a preprocessed plant sample into the sample basket; 2) placing the sample basket with the preprocessed plant sample on the bottom of the sample room of the critical point dryer, closing the sample room, performing inflation and exhaustion twice, and after final inflation, replacing the isoamyl acetate in the plant sample by CO2; and 3) adjusting the temperature of the sample room of the critical point dryer to 35-40 DEG C, discharging the CO2, taking the sample out, and the dried sample is obtained. The experiment of the young plant sample critical point drying method proves a fact that the young plant sample critical point can realize a purpose of drying the sample without deformation; and the drying effect is remarkable for a plant tissue which is tender and soft and easily deforms after shrinkage.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a critical point drying method for young plant samples used in a scanning electron microscope. Background technique [0002] In recent years, with the continuous development of science and technology, the development of Scanning Electron Microscope (SEM) has become more and more perfect, and its application in various professional fields has become more and more extensive. At present, SEM has become a powerful tool for studying the microcosm, and many major discoveries and research results are obtained through SEM observation. Although Environmental Scanning Electron Microscope (ESEM) and Cryo-Scanning Electron Microscope (Cryo-SEM) have appeared in recent years, which can observe fresh aqueous samples, the disadvantage is that the resolution is low, so currently The most used is to observe the SEM of the non-aqueous sample. [0003] As far as plant samples are concerned, only a ve...

AI Technical Summary

Problems solved by technology

Although Environmental Scanning Electron Microscope (ESEM) and Cryo-Scanning Electron Microscope (Cryo-SEM) have appeared in recent years, which can observe fresh aqueous samples, the disadvantage is that the resolution is low, so currently The most used is to observe the SEM of the non-aqueous sample

Or lead to critical point rise, drying failure

When there is too little isoamyl acetate remaining on the sample, the volatilization of isoamyl acetate will make the sample dry in the air, causing the sample to shrink

Ethanol can also be used as an intermediate solution, but it is taken out from ethanol and directly used for critical point drying, and the shrinkage of the sample is greater than that of isoamyl acetate as the intermediate solution.

Acetone can also be used as the intermediate solution, but acetone is volatile, and the sample may be air-dried in the sample basket

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