Triple marker used for diagnosing breast cancer and application thereof

A technology of phosphoribosyl and base transfer, applied in the field of clinical diagnosis

Active Publication Date: 2015-10-21
JINSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report about the combination of NAMPT, VEGF and HER2 in the diagnosis of breast cancer to improve the diagnosis rate of breast cancer

Method used

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  • Triple marker used for diagnosing breast cancer and application thereof
  • Triple marker used for diagnosing breast cancer and application thereof
  • Triple marker used for diagnosing breast cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Comparison of the expression of NAMPT, VEGF and HER2 in breast tissue samples, including 20 cases of breast benign tumors and 48 cases of breast malignant tumors

[0030] 20 cases of benign breast tumor tissues and 48 cases of breast malignant tumor tissues were selected, and the expression of NAMPT, VEGF and HER2 in tumor tissues were detected by immunohistochemical staining method, and the primary antibodies were anti-NAMPT, anti-VEGF and anti-HER2 antibodies respectively Under the light microscope, the diagnosis is given according to the depth of staining (-, 1+, 2+, 3+). Those less than or equal to 1+ are negative, and those greater than or equal to 2+ are positive. SPSS 21.0 software was used for statistical analysis, and the count data was analyzed by χ 2 Test, correlation analysis using Spearman correlation analysis, Pfigure 1 .

[0031] Table 1 The expression of NAMPT, VEGF and HER2 in benign and malignant breast tumors

[0032]

[0033] The posit...

Embodiment 2

[0034] Example 2 Comparison of the expression of NAMPT, VEGF and HER2 in the serum of breast cancer patients before and after surgery

[0035] The clear peripheral venous blood of patients with malignant breast cancer on the day before operation and the seventh day after operation (30 cases), the peripheral venous blood of patients with benign breast tumors on the day before operation (28 cases), and healthy people with similar median age and body mass index were collected respectively. The peripheral venous blood of the control group (30 cases), all blood samples were collected from the subjects’ cubital vein in the morning on an empty stomach, 4ml was drawn from each venous blood sample, centrifuged for 10 minutes, the serum was drawn and packed and frozen at -80°C Saved for inspection. Enzyme-linked immunosorbent assay (ELISA) was used to detect the concentrations of NAMPT, VEGF and HER2 in the blood, and the relationship between them and clinicopathological parameters was ...

Embodiment 3

[0040] Example 3 Comparison of correlation between plasma concentration and tissue staining of NAMPT, VEGF and HER2 in breast cancer patients before and after surgery

[0041] Comparison of the correlations between plasma NAMPT, VEGF and HER2 concentrations and tissue staining in patients with breast cancer before and after surgery Figure 4 . Regardless of the staining depth of NAMPT and VEGF in breast cancer tissues, their plasma concentrations were higher before surgery than after surgery ( Figure 4 A and Figure 4 B). When the staining of HER2 in breast cancer tissue was light, its plasma concentration was higher before surgery than after surgery; while when HER2 was stained deeply in breast cancer tissue, there was no significant difference in plasma concentration between before surgery and after surgery ( Figure 4 C).

[0042] Regardless of age, tumor size, clinical stage, lymph node metastasis, etc., the plasma concentrations of NAMPT and VEGF before surgery were ...

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Abstract

The invention relates to application of a diagnosis marker for diagnosing the breast cancer. Nicotinamide phosphoribosyltransferase (NAMPT), vascular endothelial growth factors (VEGF) and human epidermal growth factor receptor-2 (HER2) are combined to serve as the diagnosis marker. The positive rate of NAMPT in single immunohistochemical pathological diagnosis on the breast cancer is 54%, the positive rate of VEGF in single immunohistochemical pathological diagnosis on the breast cancer is 65%, and the positive rate of HER2 in single immunohistochemical pathological diagnosis on the breast cancer is 60%. The positive rates are low in single diagnosis on the breast cancer for the NAMPT, VEGF and HER2; the positive rate of diagnosis on the breast cancer through combination of the NAMPT, VEGF and HER2 is 90%. An enzyme linked immunosorbent assay (ELISA) is adopted for detecting expression conditions in serum before and after an operation performed on a patient with the breast cancer, and the detection result shows that NAMPT, VEGF and HER2 before the operation performed on the patient with the breast cancer are 6.64 times, 1.76 times and 2.52 times that of a health control group respectively (p value is smaller than 0.001), and after the operation, NAMPT, VEGF and HER2 are all decreased.

Description

technical field [0001] The invention relates to the technical field of clinical diagnosis, in particular to a triple marker for diagnosing breast cancer and its application. Background technique [0002] Breast cancer is a malignant tumor derived from mammary ductal epithelial cells, and its incidence has been on the rise. According to the estimates of the World Health Organization, about 1.4 million women are diagnosed with breast cancer and 400,000 die of breast cancer each year. The "2013 China Cancer Registration Annual Report" released by China pointed out that the incidence of breast cancer ranks first among female malignant tumors, accounting for 16.97% of all cancers; most breast cancer patients have developed to advanced stages when they go to the doctor, and they are supplemented by chemotherapy after surgery. Treatment is ineffective. Regarding breast cancer diagnostic methods, it mainly relies on imaging diagnosis and self-examination of breast lumps. At presen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57484
Inventor 许国雄朱艳艳张凌云
Owner JINSHAN HOSPITAL FUDAN UNIV
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