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Application of flavourzyme for fermenting and upgrading a cigar

A flavor protease and cigar tobacco technology, applied in the field of tobacco processing, can solve the problems of inability to decompose protein, low sugar-to-nitrogen ratio, and poor aroma quality

Inactive Publication Date: 2015-10-28
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because cigar tobacco leaves have the characteristics of low sugar-to-nitrogen ratio and high protein content, ordinary protease treatment methods are not suitable, and cannot fully decompose the protein in cigar tobacco leaves
Moreover, at present, almost all proteases act on tobacco leaves by making a solution and spraying them directly. The enzyme in the solution enters the tobacco leaves through the pores on the surface of the tobacco leaves, and can only process the surface protein of the tobacco leaves but cannot reach the depth. Due to the high protein content of cigar leaves The particularity of tobacco leaves, a large amount of protein deep inside the tobacco leaves cannot be decomposed into small molecules, which will affect the aroma and taste, make the aroma quality worse, and increase the irritation

Method used

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  • Application of flavourzyme for fermenting and upgrading a cigar
  • Application of flavourzyme for fermenting and upgrading a cigar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The application of flavor protease in cigar tobacco fermentation and quality improvement, the steps are as follows:

[0028] Flavor protease raw material is dissolved in pure water, and 10kg enzyme solution is prepared, and the enzymatic activity in the obtained enzyme solution is 1750U / ml (the density of obtained enzyme solution is 10 according to the density of pure water). 3 kg / m 3 calculate).

[0029] Vacuum the upper part of the above-mentioned cigar filler, humidify the tobacco leaves to a moisture content of 15%, soak the handle in 7kg of enzyme solution for 30 minutes, and then spray 3kg of enzyme solution (volume 3000ml) evenly on 30kg of tobacco leaves, that is, the enzyme solution The ratio of spray mass to tobacco leaf mass is 1:10, and the flavor protease activity corresponding to the enzyme solution spray in each gram of tobacco leaf (raw tobacco) is 175U. After standing still for 24 hours, after the moisture is balanced, the moisture content of the toba...

Embodiment 2

[0032] The application of flavor protease in cigar tobacco fermentation and quality improvement, the steps are as follows:

[0033] Flavor protease raw material is dissolved in pure water, and 10kg enzyme solution is prepared, and the enzyme activity in the obtained enzyme solution is 1200U / ml (the density of obtained enzyme solution is 10 according to the density of pure water). 3 kg / m 3 calculate).

[0034] After the upper part of the above-mentioned cigar filler is re-moisturized in a vacuum, it is humidified until the moisture content of the tobacco leaves is 16%. First, soak the handle in 6.25kg of enzyme solution for 30 minutes, and then spray 3.75kg of enzyme solution on 30kg of tobacco leaves evenly, that is, the spray quality of the enzyme solution The mass ratio of the tobacco leaf to the tobacco leaf is 1:8, and the corresponding flavor protease allocated to the spray of the enzyme solution in each gram of tobacco leaf (raw tobacco) is 150U. After standing still f...

Embodiment 3

[0037] The application of flavor protease in cigar tobacco fermentation and quality improvement, the steps are as follows:

[0038] Flavor protease raw material is dissolved in pure water, and 10kg enzyme solution is prepared, and the enzymatic activity in the obtained enzyme solution is 1500U / ml (the obtained enzyme solution density is 10 according to the density of pure water). 3 kg / m 3 calculate).

[0039] After the upper part of the above-mentioned cigar filler is re-moisturized in a vacuum, it is humidified until the moisture content of the tobacco leaves is 15%, and the handle is soaked in 7.5kg of enzyme solution for 30 minutes, and then 2.5kg of enzyme solution is sprayed evenly on 30kg of tobacco leaves, that is, the spray quality of the enzyme solution The mass ratio of the tobacco leaf to the tobacco leaf is 1:12, and the flavor protease corresponding to the spray of the enzyme solution in each gram of tobacco leaf (raw tobacco) is 125U. After standing still for 1...

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Abstract

The invention discloses an application of flavourzyme for fermenting and upgrading a cigar. The process of the application comprises following steps: a. humidifying naturally-aged cigar tobacco until the water content of the cigar tobacco reaches 15-16%, and soaking the petiole of the cigar tobacco into an enzyme solution prepared from the flavourzyme and water or a buffer solution for 10-30 minutes, wherein the flavourzyme activity in the enzyme solution is 1250-1750 U / ml; b. uniformly spraying the enzyme solution on the cigar tobacco, wherein the mass ration of the enzyme solution to the cigar tobacco is 1:8-12; and c. allowing the cigar tobacco to stand for 12-24 hours, adjusting the water content of the cigar tobacco after water balance is achieved, and then fermenting the cigar tobacco. After the cigar tobacco is processed through the process provided by the invention, the bitter taste of the cigar is obviously improved, the fragrance, the smoke component, the taste, the combustion performance and the grayness of the cigar are improved, and the quality of the cigar is improved.

Description

technical field [0001] The invention relates to the field of tobacco processing, in particular to the application of a flavor protease in the fermentation and quality improvement of cigar tobacco. Background technique [0002] Protein is the basic component of tobacco leaves. Its hydrolyzed products and further transformed products are the original substances of many tobacco aroma substances, but high protein content will produce a protein odor like burning feathers when smoking, and will also reduce the quality of tobacco leaves. Combustibility, and is the precursor of harmful substances in the smoke, including quinine, HCN and other nitrogen-containing compounds, which seriously affect the flavor quality and safety of tobacco leaves. [0003] At present, the most reported method for degrading excess protein in tobacco leaves is enzyme treatment. The enzymes used include neutral protease, acid protease, alkaline protease, papain, etc., among which neutral protease has a bet...

Claims

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Application Information

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IPC IPC(8): A24B15/20
CPCA24B15/20
Inventor 胡捷罗公金潘勇周斌
Owner HUBEI CHINA TOBACCO IND
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