Double cherry tissue culture propagation method
A technology of tissue culture rapid propagation and tissue culture seedlings, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of research and development of skin care products that cannot meet the needs of viewing, scarce seeds, slow seedling growth, etc.
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[0016] The specific embodiments of the present invention will be further described in detail below.
[0017] A method for rapid propagation of Yae Sakura tissue culture, characterized in that the method comprises the following steps.
[0018] (1) Selection of explants: In the morning of spring, cut the shoots of new shoots in a semi-lignified state as explants.
[0019] (2) Disinfection of explants and induction of tissue-cultured seedlings: Soak the shoot cuttings cut from the parent body in saturated bleach supernatant for 20 minutes, stir continuously during the soaking process, rinse the soaked cuttings with water, Then dry it with filter paper, put it on the ultra-clean workbench, soak it with 75% ethanol for 15 seconds, then soak the explants in 0.1% mercury liter for 8 minutes, then rinse it with sterile water for 6 times, and then inoculate into On the induction medium, the induction medium is modified MS medium with an additional 1.5 mg·L -1 6-BA, 0.1 mg L -1 NAA...
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