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Double cherry tissue culture propagation method

A technology of tissue culture rapid propagation and tissue culture seedlings, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of research and development of skin care products that cannot meet the needs of viewing, scarce seeds, slow seedling growth, etc.

Inactive Publication Date: 2015-11-04
福建世纪金花科技有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Cherry blossoms have high ornamental value. The flowers are charming and charming, and the trees are full of rosy clouds. Therefore, when the cherry blossoms are in full bloom, cherry blossom festivals will be held in various places. Cherry blossoms also have the effect of skin care. According to research, cherry blossoms can shrink pores, balance oil and It brightens the complexion and is rich in natural vitamins A, B, and E. It is often extracted and made into skin care products; however, the life of cherry blossoms is very short. It takes about 7 days for a cherry blossom to wither. Thanks a lot, it's only been about 16 days, and it can't meet people's viewing needs and the research and development of various skin care products
[0003] Yaezakura is a plant of the Rosaceae family. It is a deciduous tree, also known as "Danzakura". It is one of the categories of cherry blossoms. The leaves are ovate-lanceolate, 8-12 cm long, serrated, and alternate; the flowers are large, dark red, and several. Clustered, hanging and open, multiple and dense petals, light fragrance, long and smooth pedicel; growth characteristics: like sunshine and cool environment, blooming in March-April; mainly grown in Japan, among which Nara Yae Sakura is the most famous, currently in my country It has also begun to be planted in various places as a garden landscape for people to watch; Yaezakura is a double-petaled cherry blossom with few seeds. According to the data, the tissue culture of cherry blossoms has been successful, but the efficiency of the tissue culture technology of the Yae cherry variety is not ideal at present.

Method used

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Embodiment Construction

[0016] The specific embodiments of the present invention will be further described in detail below.

[0017] A method for rapid propagation of Yae Sakura tissue culture, characterized in that the method comprises the following steps.

[0018] (1) Selection of explants: In the morning of spring, cut the shoots of new shoots in a semi-lignified state as explants.

[0019] (2) Disinfection of explants and induction of tissue-cultured seedlings: Soak the shoot cuttings cut from the parent body in saturated bleach supernatant for 20 minutes, stir continuously during the soaking process, rinse the soaked cuttings with water, Then dry it with filter paper, put it on the ultra-clean workbench, soak it with 75% ethanol for 15 seconds, then soak the explants in 0.1% mercury liter for 8 minutes, then rinse it with sterile water for 6 times, and then inoculate into On the induction medium, the induction medium is modified MS medium with an additional 1.5 mg·L -1 6-BA, 0.1 mg L -1 NAA...

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Abstract

The invention provides a double cherry tissue culture propagation method. An economical efficient double cherry tissue culture propagation technology system is established according to the steps of explant selection, explant sterilization, tissue culture seedling induction, tissue culture seedling proliferation and tissue culture seedling rooting. An improved MS culture medium is adopted, the purposes of improving the double cherry transplantation survival rate, ensuring high propagation coefficient and short period and realizing rapid-propagation seedling culture without external environment influence can be achieved, the excellent characteristics of excellent varieties can be preserved, and the problem in short supply of double cherry is alleviated.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation, in particular to a method for tissue culture and rapid propagation of Yae cherry, which is suitable for the rapid propagation of Yae cherry. Background technique [0002] Cherry blossoms have high ornamental value. The flowers are charming and charming, and the trees are full of rosy clouds. Therefore, when the cherry blossoms are in full bloom, cherry blossom festivals will be held in various places. Cherry blossoms also have the effect of skin care. According to research, cherry blossoms can shrink pores, balance oil and It brightens the complexion and is rich in natural vitamins A, B, and E. It is often extracted and made into skin care products; however, the life of cherry blossoms is very short. It takes about 7 days for a cherry blossom to wither. Thank you for only about 16 days, which cannot meet people's viewing needs and the research and development of various skin ca...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 樊仲书曾毓张玉华陈天增樊昌华
Owner 福建世纪金花科技有限公司
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