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Embryo-strongly expressed plant seed-specific expression promoter ossee2

A technology of plant seeds and promoters, applied in the fields of biotechnology and plant genetic engineering, can solve the problems of lack of strong embryo promoters, reports, not many, etc., and achieve the effects of increasing nutritional value and improving expression.

Inactive Publication Date: 2018-05-11
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there are not many studies on promoters in rice embryos, and there are no reports on embryo-strong promoters.

Method used

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  • Embryo-strongly expressed plant seed-specific expression promoter ossee2
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  • Embryo-strongly expressed plant seed-specific expression promoter ossee2

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Experimental program
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Embodiment Construction

[0040] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0041] The experimental methods in the following examples are conventional methods unless otherwise specified. The medicinal raw materials, reagent materials, etc. used in the following examples are all commercially available products unless otherwise specified.

[0042] Acquisition of OsSee2 promoter containing restriction site

[0043] Step 1. Design of primers

[0044] According to the whole genome sequence of rice variety Nipponbare (Oryza sativa L cv. Nipponbare) provided by NCBI, the amplification primers were designed according to the sequence of the rice OsSee2 gene, and the restriction sites of the primers were designed according to the characteristics of the selected vector and target gene.

[0045] I...

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PUM

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Abstract

The invention discloses an embryo-strongly expressed plant seed-specific expression promoter OsSee2 and its application. The promoter of the present invention is based on the sequences in SEQ ID No: 1 and 2 in the sequence listing, and is extended on this basis. The present invention includes various variants of the above two sequences, especially the sequence with promoter function in the various variants. The invention also provides an expression cassette and a plant expression vector containing the plant seed-specific expression promoter OsSee2 strongly expressed by the embryo and applies it to plant genetic engineering. In addition, the present invention also provides a set of primer pairs specially used for amplifying the promoter of the present invention from Nipponbare rice. The promoter of the invention has important theoretical and practical significance for the study of rice seed-related molecular mechanisms.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to an embryo-strongly expressed plant seed-specific expression promoter OsSee2. Background technique [0002] Rice is currently one of the most important food crops for humans, and more than one-third of the world's population uses rice as a staple food. With the development of the economy, there are higher and higher requirements on the yield and quality of rice worldwide. Therefore, it is of great significance to use various methods to increase the yield per unit area of ​​rice and improve the quality of rice. Researching and improving crops by means of molecular biology and genetic engineering has a good application prospect in agricultural production. In recent years, the research on plant genetic engineering has made rapid progress and has become an important method and technology in modern biology and breedi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/11C12N15/82A01H5/00A01H6/46
Inventor 李浩杨剑波李莉魏鹏程杨亚春秦瑞英马卉许蓉芳
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI