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Kit used for detecting polymorphism of genes related to Warfarin and Clopidogrel personalized medication and application thereof

A technology of clopidogrel and polymorphism, applied in the determination/testing of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc.

Active Publication Date: 2015-11-04
BOAO BIOLOGICAL CO LTD +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no product in China that can simultaneously detect the drug-resistant genotypes associated with two drugs

Method used

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  • Kit used for detecting polymorphism of genes related to Warfarin and Clopidogrel personalized medication and application thereof
  • Kit used for detecting polymorphism of genes related to Warfarin and Clopidogrel personalized medication and application thereof
  • Kit used for detecting polymorphism of genes related to Warfarin and Clopidogrel personalized medication and application thereof

Examples

Experimental program
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Embodiment 1

[0111] Example 1. Using the universal chip and method provided by the present invention to detect the SNP typing of warfarin and clopidogrel individualized drug-related genes in case samples

[0112] 1. Acquisition of clinical samples and extraction of DNA

[0113] The case samples (whole blood, sequenced to verify the mutation typing of related genes) of known cardiovascular disease individualized drug-related gene mutation typing were provided by the Department of Thrombosis and Cardiovascular Research, Fuwai Cardiovascular Hospital, Chinese Academy of Medical Sciences. use Genomic DNA Purification Kit (Promega, Madison, USA) was used to extract genomic DNA from whole blood.

[0114] 2. Preparation of primers and probes

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Abstract

The invention discloses a kit used for detecting the polymorphism of genes related to Warfarin and Clopidogrel personalized medication and application thereof. The kit comprises a general chip, a first primer combination and a second primer combination, wherein each primer combination unit in the first primer combination is used for identifying one polymorphism site of the genes related to Warfarin medicine resistance, and each primer combination unit in the second primer combination is used for identifying one polymorphism site of the genes related to Clopidogrel medicine resistance. The kit has the advantages of being high in integration degree, sensitivity, specificity, reliability and operability and wide in application range, detection results are stable, a use method is fast and convenient, and automation of the kit is easy to achieve; the kit can be used for detecting gene mutation, analyzing gene polymorphism and the like and is suitable for gene analysis fields such as mutation detection of clinical diseases, pharmacogenomics analysis and medicolegal expertise.

Description

technical field [0001] The invention relates to a kit for detecting polymorphisms of genes related to warfarin and clopidogrel individualized drug use and an application thereof. Background technique [0002] Warfarin is a coumarin-based oral anticoagulant that was synthesized by the University of Wisconsin in the United States in the 1940s. At present, warfarin is widely used in the prevention and treatment of venous thrombosis, pulmonary embolism, chronic atrial fibrillation, myocardial infarction, ischemic shock and other thrombotic diseases and artificial heart valve implantation operations. Other currently approved oral anticoagulants. However, due to its narrow therapeutic index, large individual differences in dosage and risk of bleeding, warfarin ranks among the top ten drugs for serious adverse drug reactions. With the progress of pharmacogenetics research, scholars at home and abroad have found that genetic factors are the main reason for individual differences i...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 项光新梁书剑邢婉丽
Owner BOAO BIOLOGICAL CO LTD
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