Kit used for detecting polymorphism of genes related to Warfarin and Clopidogrel personalized medication and application thereof
A technology of clopidogrel and polymorphism, applied in the determination/testing of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc.
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[0111] Example 1. Using the universal chip and method provided by the present invention to detect the SNP typing of warfarin and clopidogrel individualized drug-related genes in case samples
[0112] 1. Acquisition of clinical samples and extraction of DNA
[0113] The case samples (whole blood, sequenced to verify the mutation typing of related genes) of known cardiovascular disease individualized drug-related gene mutation typing were provided by the Department of Thrombosis and Cardiovascular Research, Fuwai Cardiovascular Hospital, Chinese Academy of Medical Sciences. use Genomic DNA Purification Kit (Promega, Madison, USA) was used to extract genomic DNA from whole blood.
[0114] 2. Preparation of primers and probes
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