A kind of dna chip and detection method thereof
A technology of DNA chips and substrates, applied in the field of DNA chips, can solve problems such as the absence of gene chips
Active Publication Date: 2018-04-13
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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However, there is no gene chip designed using SERS technology
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[0020] In order to make the objectives, technical solutions and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with implementation examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0021] Firstly, it is stated that there are three types of DNA in the present invention: probe DNA, target DNA, and reporter DNA. The probe DNA is used to capture the target DNA, and the reporter DNA is used to send out detection signals.
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The invention discloses a DNA chip, a detection method and a production method thereof. The substrate of the DNA chip is a SERS substrate with a noble metal nanoarray as the substrate; the noble metal nanoarray is divided into several regions, and the surface of each region is modified and connected with a number of different DNA probes to make the DNA chip contain Different probe DNA chips use these probe DNA to detect the target DNA to generate SERS signals, and realize the recognition and quantitative detection of the target DNA according to the frequency change and amplitude difference of the SERS signal. The DNA chip is a DNA chip designed for the use of noble metal nanoarrays and surface-enhanced Raman spectroscopy (SERS) technology to achieve the detection and analysis of target DNA and genes. The invention also discloses a detection method and a production method of the DNA chip.
Description
technical field [0001] The invention relates to the fields of biological detection, biotechnology and nanotechnology, in particular to a DNA chip, a detection method of the DNA chip, and a manufacturing method of the DNA chip. Background technique [0002] DNA chip, also known as gene chip (genechip), is a kind of biochip, which refers to immobilizing a large number of probe molecules on a support, and hybridizing with labeled sample molecules according to the principle of complementary base pairing, and detecting the strength of the hybridization signal and distribution to obtain the sequence and quantity information of the target molecule. Supports for DNA chips include glass slides, silicon wafers, NC membranes, Nylon membranes, etc. DNA chip technology can be widely used in many fields such as disease diagnosis and treatment, optimal breeding of crops, judicial identification, food hygiene supervision, and environmental testing. The current DNA chip detection technolog...
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IPC IPC(8): C40B40/06C40B50/06C12Q1/6837G01N21/65
Inventor 黄青孙克喜孟国文
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI