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Insect nematode moisturizing agent and insect nematode preservation method

A preservation method and humectant technology, applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of toxicity of mineral oil, the survival rate is greatly affected by the environmental temperature and humidity, and the nozzle is easy to block.

Inactive Publication Date: 2017-04-05
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although insect nematode is a new biopesticide with broad application prospects, its survival rate is greatly affected by environmental temperature and humidity, it is sensitive to light, and its shelf life is short when stored at room temperature. These shortcomings are the main obstacles to its popularization and application.
In the practice of using insect nematodes to control pests, scholars at home and abroad usually take the low-temperature stored insect nematode aqueous suspension stock solution to the field for dilution on the same day, and then apply it immediately. This operation method that cannot be separated from low temperature has brought great benefits to farmers. It is inconvenient and difficult to promote on a large scale, especially in subtropical and tropical areas such as Guangdong and Hainan
For this reason, insect nematode workers have studied the use of various materials as carriers or auxiliary agents to prepare artificially cultivated insect nematodes to meet the requirements of laboratory stock preservation, industrialization and commercialization of products, but these formulations There are still some problems in the survival rate, preservation, transportation and application of nematode products
For example, low-temperature water or liquid nitrogen is used to store species in the laboratory, but this method has the disadvantages of low temperature, difficulty in feeding, limited storage concentration, and poor applicability; making oil with mineral oil or storing it with sponge method, although the raw materials are easy However, mineral oil is toxic, and it is difficult to supply oxygen for long-term storage in large quantities; the loss of nematode spongiosa is large, and these two formulations are limited to small-scale storage on a laboratory scale
In the mass storage method commercially, nematodes are adsorbed and dehydrated by the adsorbent method. Although this method is simple to operate and the raw materials are easy to obtain, its storage stability is poor and the nozzles are easily blocked; the gel storage method is easy to operate and can partially dehydrate the nematodes. The storage stability is good, but its oxygen supply is difficult, the process is complicated, the release of nematodes is troublesome, and the nozzle is easy to be blocked

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, insect nematode R. chongmingensis Tumian storage effect under room temperature in humectant

[0030] 1 Materials and methods

[0031] 1.1 Experimental materials

[0032] Insect nematodes to be tested: Rhabditis (Oscheius) chongmingensis Tumian.

[0033] 1.2 Test method

[0034]The small rod family nematode Rhabditis (Oscheius) chongmingensis Tumian was prepared into insect nematode moisturizer compositions with five concentrations of 2000IJs / mL, 4000IJs / mL, 6000IJs / mL and 10000IJs / mL. Take 15ml of the insect nematode moisturizer composition and evenly add it to a plastic petri dish with a bottom diameter of 5.5cm, set it as one repetition, and set up 3 repetitions in total, and place it at natural room temperature for 2-6 months.

[0035] About 50 nematodes were randomly selected from the petri dish every week, and examined under a stereomicroscope. The nematodes were regarded as dead if they were dead and unresponsive to acupuncture. The number of deat...

Embodiment 2

[0040] Embodiment 2, insect nematode Heterorhabditis beicherria storage effect under room temperature in humectant

[0041] 1 Materials and methods

[0042] 1.1 Experimental materials

[0043] Insect nematodes for testing: Heterorhabditis beicherria.

[0044] 1.2 Test method

[0045] The Heterorhabditis beicherria nematode Heterorhabditis beicherria was used to prepare insect nematode moisturizer compositions with five concentrations of 2000IJs / mL, 4000IJs / mL, 6000IJs / mL and 10000IJs / mL. Take 15ml of the insect nematode moisturizer composition and evenly add it to a plastic petri dish with a bottom diameter of 5.5cm, set it as one repetition, and set up 3 repetitions in total, and place it at natural room temperature for 2-6 months.

[0046] About 50 nematodes were randomly selected from the petri dish every week, and examined under a stereomicroscope. The nematodes were regarded as dead if they were dead and unresponsive to acupuncture. The number of deaths was observed an...

Embodiment 3

[0051] Embodiment 3, insect nematode Steinernema carpocapsae storage effect under room temperature in humectant

[0052] 1 Materials and methods

[0053] 1.1 Experimental materials

[0054] Insect nematodes for testing: Steinernema carpocapsae of the Steinernema carpocapsae family.

[0055] 1.2 Test method

[0056] Five concentrations of 2000IJs / mL, 4000IJs / mL, 6000IJs / mL, and 10000IJs / mL were prepared from Steinernema carpocapsae, which were respectively loaded into moisturizers to prepare insect nematode moisturizer compositions. Take 15ml of the insect nematode moisturizer composition and evenly add it to a plastic petri dish with a bottom diameter of 5.5cm, set it as one repetition, and set up 3 repetitions in total, and place it at natural room temperature for 2-6 months. About 50 nematodes were randomly selected from the petri dish every week, and examined under a stereomicroscope. The nematodes were regarded as dead if they were dead and unresponsive to acupuncture. ...

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Abstract

The invention relates to an insect nematode humectant and an insect nematode preservation method, and belongs to the field of biological control. The insect nematode humectant is a viscous aqueous solution composed of a solid being insect body, an insect metabolite, oligosaccharides and a polymer material (polyacrylic polymer [(C3H3O2Na)0.7(C3H4O2)0.3]n), and the above viscous preparation can provide nutrition and humidity environment for insect nematodes, and prolongs the preservation time of the insect nematodes. Insect nematodes are inoculated to the viscous preparation, so the insect nematodes can be preserved at normal temperature for 2-5 months, and the viscous preparation is suitable for transportation and preservation of the insect nematodes at normal temperature, realizes no inactivation of the insect nematodes, and has no side effects on applied soil.

Description

technical field [0001] The invention relates to a moisturizing agent capable of long-term preservation of insect nematodes at room temperature and is convenient to carry and use, and provides a method for preserving nematodes by using the moisturizing agent. Background technique [0002] Insect nematodes are nematodes that can parasitize in insects. According to whether there are symbiotic bacteria involved in the infection process, it can be divided into two categories: entomopathogenic nematodes and insect parasitic nematodes. Insect nematodes belong to the phylum Nemathelminthes and the class Nematoda, mainly including 8 orders such as Rhabditida and Mermithida; Steinernematidae, Heterorhabditidae, There are 28 families including Rhabditidae. Among them, Entomopathogenic nematodes (EPNs) mainly include Steinernematidae and Heterorhabditidae nematodes. Among the insect parasitic nematodes, there are many researches on Rhabditidae nematodes. [0003] Insect nematodes ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 刘奇志魏太阳
Owner CHINA AGRI UNIV