A microfluidic chip with controllable generation of stable concentration gradient

Abstract

The invention discloses a micro-fluidic chip capable of generating stable concentration gradient in a controlled manner. The micro-fluidic chip is composed of a first PDMS film layer, a second PDMS film layer and a glass substrate, wherein the first PDMS film layer, the second PDMS film layer and the glass substrate are sequentially subjected to irreversible bonding to form an integral structure, the first PDMS film layer is composed of a plurality of groups of micro-valves, and control channels of the micro-valves, each group of micro-valves comprises two micro-valves, the second PDMS film layer comprises a first liquid inlet, a second liquid inlet, a first liquid outlet, a second liquid outlet, a first main fluid passage, a second main fluid passage, a first branch fluid passage unit, a second branch fluid passage unit, a first liquid passage and a second liquid passage. According to the micro-fluidic chip, by controlling the opening and closing of the micro-valves, different concentrations are generated on the chip, meanwhile, the micro-fluidic chip can simultaneously act on two downstream chips for experiments, the generation of air bubbles is effectively avoided, and the problem that the generated concentration is not stable is solved.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a microfluidic chip that can controllably generate a stable concentration gradient. Background technique [0002] Generating concentration gradients in microfluidic chips is one of the greatest advantages of microfluidic chips for drug screening and biochemical reactions. Deformation structure, the basic principle is: when two fluids with different concentrations pass through the designed microchannel network, after the solutions are separated and mixed repeatedly, each branch contains different proportions of the original solution, and the final solution is in the Christmas tree. A concentration gradient is formed at the bottom of the network structure. However, there are also certain problems in the microfluidic chip with such a structure. For example, air bubbles are easily generated in the liquid channels, resulting in different liquid volumes in different channel...

AI Technical Summary

Problems solved by technology

However, there are also certain problems in the microfluidic chip with such a structure. For example, air bubbles are easily generated in the liquid channels, resulting in different liquid volumes in different channels, resulting in inaccurate concentrations; If the length is large, once one of the fluid channels is blocked at any position, the concentration gradient generated in the entire chip will no longer be accurate; when more concentration gradients are required, the chip volume will increase with the number of "Christmas tree" layers. It is greatly increased, and if only one concentration of one of the outlets is used, the liquid produced by other outlets can only flow out and become waste liquid, resulting in increased cost, decreased experimental accuracy and repeatability

Images