Method and system for nanometer optical coherence tomography (OCT) imaging based on broadband stimulated radiation

A technology of stimulated radiation and imaging methods, which is applied in the field of nano-OCT imaging methods and systems, can solve problems such as undetectable and weak autofluorescence, and achieve the effects of real-time observation, ultra-high lateral resolution, and improved imaging speed

Active Publication Date: 2015-11-11
ZHEJIANG UNIV
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Problems solved by technology

The electrons in such substances will still transition to the excited state after absorbing the excitation light photons, but then most of them will return to the ground state in a non-radiative way, resulting in extremely weak autofluorescence that cannot be detected

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  • Method and system for nanometer optical coherence tomography (OCT) imaging based on broadband stimulated radiation
  • Method and system for nanometer optical coherence tomography (OCT) imaging based on broadband stimulated radiation
  • Method and system for nanometer optical coherence tomography (OCT) imaging based on broadband stimulated radiation

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with the accompanying drawings and implementation examples.

[0026] A nano-OCT imaging method based on broadband stimulated radiation:

[0027] 1) Turn on the excitation light to irradiate the sample, so that the sample is in an excited state;

[0028] 2) When the excitation light is turned on, the lost light is turned on at the same time; the lost light is modulated by the beam to form a transverse hollow telephoto spot; the lost light is irradiated on the sample, and the excited state electrons located on the periphery of the exciting spot will pass through the stimulated radiation. way to return to the ground state, and the excited state electrons near the center of the excitation spot will remain in the excited state;

[0029] 3) Turn off the excitation light and loss light, turn on the probe light, and send the electrons left in the excited state back to the ground state by means of stimulated r...

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Abstract

The invention discloses a method and system for nanometer optical coherence tomography (OCT) imaging based on broadband stimulated radiation. A chromophore positioned in an excited state is caused to quickly return to a ground state by utilizing the stimulated radiation rather than spontaneous radiation, and a broadband detection light source is adopted to achieve coherent detection of stimulated radiation fluorescence signals. The improvement of axial and transverse resolutions is achieved through a broadband light source technology and a stimulated radiation loss technology respectively. An ultra-broadband light source is adopted to serve as a detection light source, and the axial resolution from a submicron grade to a hundred-nanometer grade can be achieved. The nanoscale transverse resolution can be achieved by adopting a point spread function regulation technology based on stimulated radiation loss. Compared with conventional fluorescence imaging, according to the imaging method, the imaging speed can be greatly improved. According to the method and system for nanometer OCT imaging based on broadband stimulated radiation, not only is the nanometer resolution of the OCT imaging achieved, but also a fluorescence imaging function of the OCT is expanded, and the method is provided for optical imaging of the non-fluorescent chromophore.

Description

technical field [0001] The present invention relates to OCT technology, stimulated radiation detection technology and stimulated radiation depletion (Stimulated Emission Depletion, STED) microscopy, in particular to a nanometer OCT imaging method and system based on broadband stimulated radiation. technical background [0002] With the rapid development of the field of biomedicine, nano-resolution technology has been paid more and more attention by researchers. On the one hand, in order to better understand the process of human life and the formation mechanism of diseases, people need to observe the precise positioning and distribution of intracellular organs, viruses, parasites, etc. in three-dimensional cellular space. On the other hand, the research on protein science in the post-genome era also requires elucidation of the relationship between protein structure, location and function, and the temporal and spatial sequence of protein-protein interactions; biological macrom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 丁志华唐弢
Owner ZHEJIANG UNIV
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