Method and system for nanometer optical coherence tomography (OCT) imaging based on broadband stimulated radiation

Abstract

The invention discloses a method and system for nanometer optical coherence tomography (OCT) imaging based on broadband stimulated radiation. A chromophore positioned in an excited state is caused to quickly return to a ground state by utilizing the stimulated radiation rather than spontaneous radiation, and a broadband detection light source is adopted to achieve coherent detection of stimulated radiation fluorescence signals. The improvement of axial and transverse resolutions is achieved through a broadband light source technology and a stimulated radiation loss technology respectively. An ultra-broadband light source is adopted to serve as a detection light source, and the axial resolution from a submicron grade to a hundred-nanometer grade can be achieved. The nanoscale transverse resolution can be achieved by adopting a point spread function regulation technology based on stimulated radiation loss. Compared with conventional fluorescence imaging, according to the imaging method, the imaging speed can be greatly improved. According to the method and system for nanometer OCT imaging based on broadband stimulated radiation, not only is the nanometer resolution of the OCT imaging achieved, but also a fluorescence imaging function of the OCT is expanded, and the method is provided for optical imaging of the non-fluorescent chromophore.

Description

technical field [0001] The present invention relates to OCT technology, stimulated radiation detection technology and stimulated radiation depletion (Stimulated Emission Depletion, STED) microscopy, in particular to a nanometer OCT imaging method and system based on broadband stimulated radiation. technical background [0002] With the rapid development of the field of biomedicine, nano-resolution technology has been paid more and more attention by researchers. On the one hand, in order to better understand the process of human life and the formation mechanism of diseases, people need to observe the precise positioning and distribution of intracellular organs, viruses, parasites, etc. in three-dimensional cellular space. On the other hand, the research on protein science in the post-genome era also requires elucidation of the relationship between protein structure, location and function, and the temporal and spatial sequence of protein-protein interactions; biological macrom...

AI Technical Summary

Problems solved by technology

The electrons in such substances will still transition to the excited state after absorbing the excitation light photons, but then most of them will return to the ground state in a non-radiative way, resulting in extremely weak autofluorescence that cannot be detected

Images