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A nano-oct imaging method and system based on broadband stimulated radiation

A technology of stimulated radiation and imaging methods, applied in the field of nano-OCT imaging methods and systems, can solve problems such as undetectable and weak autofluorescence, and achieve the effects of real-time observation, ultra-high lateral resolution, and reduced lateral size

Active Publication Date: 2017-10-13
ZHEJIANG UNIV
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Problems solved by technology

The electrons in such substances will still transition to the excited state after absorbing the excitation light photons, but then most of them will return to the ground state in a non-radiative way, resulting in extremely weak autofluorescence that cannot be detected

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  • A nano-oct imaging method and system based on broadband stimulated radiation
  • A nano-oct imaging method and system based on broadband stimulated radiation
  • A nano-oct imaging method and system based on broadband stimulated radiation

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with the accompanying drawings and implementation examples.

[0026] A nano-OCT imaging method based on broadband stimulated radiation:

[0027] 1) Turn on the excitation light to irradiate the sample, so that the sample is in an excited state;

[0028] 2) When the excitation light is turned on, the lost light is turned on at the same time; the lost light is modulated by the beam to form a transverse hollow telephoto spot; the lost light is irradiated on the sample, and the excited state electrons located on the periphery of the exciting spot will pass through the stimulated radiation. way to return to the ground state, and the excited state electrons near the center of the excitation spot will remain in the excited state;

[0029] 3) Turn off the excitation light and loss light, turn on the probe light, and send the electrons left in the excited state back to the ground state by means of stimulated r...

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Abstract

The invention discloses a nano OCT imaging method and system based on broadband stimulated radiation. The invention uses stimulated radiation instead of spontaneous radiation to quickly return the chromophore in the excited state to the ground state, and uses a broadband detection light source to realize the coherent detection of the stimulated radiation fluorescence signal. The improvement of axial and lateral resolution is achieved by broadband light source technology and stimulated radiation loss technology, respectively. The ultra-broadband light source is used as the detection light source, and the axial resolution from submicron to hundreds of nanometers can be achieved. Using point spread function control technology based on stimulated radiation loss, nanometer-scale lateral resolution can be achieved. In view of the fact that the stimulated radiation process is much shorter than the spontaneous radiation process, the imaging speed of the imaging method of the present invention can be greatly improved compared with the traditional fluorescence imaging. The invention not only realizes the nanometer resolution of OCT imaging, but also expands the fluorescent imaging function of OCT, and provides a method for optical imaging of non-fluorescent chromophores.

Description

technical field [0001] The present invention relates to OCT technology, stimulated radiation detection technology and stimulated radiation depletion (Stimulated Emission Depletion, STED) microscopy, in particular to a nano OCT imaging method and system based on broadband stimulated radiation. technical background [0002] With the rapid development of the field of biomedicine, nano-resolution technology has been paid more and more attention by researchers. On the one hand, in order to better understand the process of human life and the formation mechanism of diseases, people need to observe the precise positioning and distribution of intracellular organs, viruses, parasites, etc. in three-dimensional cellular space. On the other hand, the research on protein science in the post-genome era also requires elucidation of the relationship between protein structure, location and function, and the temporal and spatial sequence of protein-protein interactions; biological macromolecu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 丁志华唐弢
Owner ZHEJIANG UNIV
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