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Three-dimensional hollow light spot generating method and device

The invention discloses a three-dimensional hollow light spot generating method and a three-dimensional hollow light spot generating device. The three-dimensional hollow light spot generating device comprises a laser, a single mode fiber, a collimating lens, a first polarizing beam splitter prism, a plurality of light turning assemblies, two phase modulators, a second polarizing beam splitter prism, a 1/4 wave plate, a microobjective, a sample surface and a medium film reflecting lens. The three-dimensional hollow light spot generating method comprises the following steps; performing different phase modulation on vertical polarized light and parallel polarized light; combining the two modulated light beams, and converting the combined light beam into two circuit polarized lights by the same 1/4 wave plate; projecting the two circuit polarized lights by the microobjective to penetrate through the sample surface, and reflecting the two circuit polarized lights by the medium film reflecting lens; interfering the reflected light irradiated to the sample surface with projecting light to form a transverse hollow light spot and an axial hollow light spot respectively; and superposing the light intensities of the two hollow light spots to form a three-dimensional hollow light spot, wherein the transverse size of the three-dimensional hollow light spot is 0.56 wavelengths and the axial size of the three-dimensional hollow light spot is 0.44 wavelengths. The three-dimensional hollow light spot generating method and the three-dimensional hollow light spot generating device can be applied to super-resolution microscopic equipment such as a stimulated emission depletion microscope and are used for realizing three-dimensional super-resolution microscopy.
Owner:ZHEJIANG UNIV
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