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LDL-C detection kit prepared through combined-covering method

A technology of LDL-C and kit, applied in the biological field, can solve the problem of high price

Active Publication Date: 2015-11-11
王贤俊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic clinical laboratories use homogeneous phase method to determine LDL-C reagents, which are mostly imported from Japan, such as the reagents produced by Ichika and Wako, or domestic reagents produced by foreign technologies, such as the products of Leadman and Shensuo. higher price

Method used

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  • LDL-C detection kit prepared through combined-covering method
  • LDL-C detection kit prepared through combined-covering method
  • LDL-C detection kit prepared through combined-covering method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Preparation of reagent 1:

[0022]

[0023] 2. Preparation of reagent 2:

[0024]

[0025] Table 2 is the result data of the LDL-C detection kit prepared in Example 1, which was continuously measured for 10 times on the Hitachi 7180 automatic biochemical analyzer under the same external conditions.

[0026] Table 2 Accuracy measurement result table

[0027] Measurement times Result (mmol / L) 1 2.91 2 2.89

[0028] 3 3.00 4 2.98 5 2.85 6 2.92 7 2.78 8 2.88 9 2.94 10 2.88 average 2.90

[0029] As can be seen from Table 2, the accuracy of the LDL-C detection kit prepared in Example 1 is: 0.69%

[0030] Table 3 shows the result data of 20 consecutive determinations of the quality control serum by the Hitachi 7180 automatic biochemical analyzer with the LDL-C detection kit in Example 1 under the same external conditions.

[0031] Table 3 precision measurement result table

[0032] ...

Embodiment 2

[0040] 1. Preparation of reagent 1:

[0041]

[0042]

[0043] 2. Preparation of reagent 2:

[0044]

[0045] Table 2 is the result data of the LDL-C detection kit prepared in Example 2, which was continuously measured for 10 times on the Hitachi 7180 automatic biochemical analyzer under the same external conditions.

[0046] Table 2 Accuracy measurement result table

[0047] Measurement times Result (mmol / L) 1 2.58 2 2.64 3 2.56 4 2.57 5 2.62 6 2.53 7 2.56 8 2.59 9 2.59 10 2.62 average 2.59

[0048] As can be seen from Table 2, the accuracy of the LDL-C detection kit prepared in Example 2 is: 0.81%

[0049] Table 3 shows the result data of 20 consecutive determinations of quality control serum by the Hitachi 7180 automatic biochemical analyzer with the LDL-C detection kit in Example 2 under the same external conditions.

[0050] Table 3 precision measurement result table

[0051] Meas...

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Abstract

The invention relates to a LDL-C detection kit prepared through a combined-covering method, wherein the kit is composed of a reagent 1 and a reagent 2 placed separately. The reagent 1 includes a metal chloride, an LDL-C monoclonal antibody and a quantitative enzyme reagent and the reagent 2 includes a surfactant, a color developing agent, a preservative, an anti-interference substance and a reaction promoter. The kit is good in color developing property, is high in accuracy, is stable in reagent components, is simple in operations, is suitable for various clinical full-automatic biochemical analyzers, and is used for detecting the content of LDL-C in human serum.

Description

Technical field: [0001] The invention relates to a detection kit for measuring LDL-C in serum, which belongs to the field of biotechnology. Background technique: [0002] Low-density lipoprotein (LDL) is the most abundant lipoprotein in human blood, and the cholesterol (LDL-C) it carries is considered to be the main pathogenic factor of atherosclerosis and coronary heart disease, and clinical detection has become increasingly important. [0003] At present, there is no real reference method for measuring LDL-C. The CDC tentative reference method for measuring LDL-C is the ultracentrifugation method (Betaquantification, β-quantitative method / BQ method), which is also recommended by NCEP. The LDL-C measured by this method actually includes the cholesterol content of lipoprotein (a) [Lp(a)] and intermediate density lipoprotein (IDL), which is also the basis for evaluating the correctness of other detection methods. This method requires expensive equipment, complicated operati...

Claims

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Application Information

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IPC IPC(8): G01N33/92G01N33/531
CPCG01N33/531G01N33/92G01N2800/323G01N2800/324
Inventor 王贤俊郭二豪郑蓓蕾江新涛
Owner 王贤俊