Preparation method of electrochemical immunosensor used for detection of estradiol and built on basis of MoS2 composite

An immune sensor and composite material technology, applied in the field of new nano functional materials and sensing and detection, can solve the problems of cumbersome process and low yield, and achieve the effect of simple preparation process, convenient operation, and improved electrochemical response signal strength

Active Publication Date: 2015-11-18
上海佑科仪器仪表有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the synthesis methods are synthesized separately, and then compound the catalyst and the carrier, the process is cumbersome and the yield is not high

Method used

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  • Preparation method of electrochemical immunosensor used for detection of estradiol and built on basis of MoS2 composite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1MoS 2 / Preparation method of FePd

[0027] Take 35mL of CTAB solution with a concentration of 0.1mol / L, add 0.02g of FeCl 3 ·6H 2 O and 2 mL of H at a concentration of 0.03 mol / L 2 PdCl 4 solution, after stirring for 15 minutes, continue to stir and add 2 mL of ascorbic acid solution with a concentration of 0.3 mol / L, 0.5 mL of Na with a concentration of 0.1 mol / L 2 MoO 4 solution and 0.01 g of Na 2 S, after stirring for 15 minutes, put it into the reaction kettle, and react for 14 hours at 180°C; after cooling to room temperature, use deionized water to wash centrifugally, and vacuum dry at 40°C to obtain MoS 2 / FePd, which was dissolved in deionized water to obtain MoS at a concentration of 1 mg / mL 2 / FePd solution.

Embodiment 2

[0028] Example 2MoS 2 / Preparation method of FePd

[0029] Take 35mL of CTAB solution with a concentration of 0.1mol / L, add 0.04g of FeCl 3 ·6H 2 O and 4 mL of H at a concentration of 0.03 mol / L 2 PdCl 4 solution, after stirring for 15 minutes, continue to stir and add 4 mL of ascorbic acid solution with a concentration of 0.3 mol / L, 1.0 mL of Na with a concentration of 0.1 mol / L 2 MoO 4 solution and 0.02g Na 2 S, after stirring for 15 minutes, put it into the reaction kettle, and react for 16 hours at 150°C; after cooling to room temperature, use deionized water to wash centrifugally, and vacuum dry at 40°C to obtain MoS 2 / FePd, which was dissolved in deionized water to obtain MoS at a concentration of 1 mg / mL 2 / FePd solution.

Embodiment 3

[0030] Example 3MoS2 / Preparation method of FePd

[0031] Take 35mL of CTAB solution with a concentration of 0.1mol / L, add 0.06g of FeCl 3 ·6H 2 O and 6 mL of H at a concentration of 0.03 mol / L 2 PdCl 4 solution, after stirring for 15 minutes, continue to stir and add 6 mL of ascorbic acid solution with a concentration of 0.3 mol / L, 1.5 mL of Na with a concentration of 0.1 mol / L 2 MoO 4 solution and 0.03g Na 2 S, after stirring for 15 minutes, put it into the reaction kettle, and react for 12 hours at 220°C; after cooling to room temperature, use deionized water to wash centrifugally, and vacuum dry at 40°C to obtain MoS 2 / FePd, which was dissolved in deionized water to obtain MoS at a concentration of 1 mg / mL 2 / FePd solution.

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Abstract

The invention discloses a preparation method of an electrochemical immunosensor used for detection of estradiol and built on the basis of MoS2 composite and belongs to the technical field of novel nano functional materials and sensing detection. A MoS2 / iron-palladium alloy nanocomposite MoS2 / FePd is prepared with a one-pot method, and then the simple electrochemical immunosensor which can be used for detecting estradiol in an environmental water sample simply, quickly and sensitively is prepared by means of the excellent adsorption and electrochemical catalytic performance of the composite.

Description

technical field [0001] The invention relates to a method for preparing a label-free electrochemical immunosensor based on inorganic nanocomposite materials, and the prepared sensor can be used for the detection of estradiol. The invention belongs to the field of new nano functional material and sensing detection technology. Background technique [0002] Estradiol is an environmental estrogen-like chemical. Environmental estrogen has the activity similar to that of biological estrogen. After entering the human body, it has the effect of simulating estrogen. By disrupting the normal functions of the human endocrine, immune, and nervous systems, it can cause cancer, damage the reproductive and developmental system, immune system, and nervous system. It is a class of environmental toxins that seriously endanger human health. Rapid and sensitive qualitative and quantitative detection of estradiol in environmental samples is of great significance to people's production and life....

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N33/74
Inventor 张勇李娇娇杜斌王耀光吴丹魏琴
Owner 上海佑科仪器仪表有限公司
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